FastQCFastQC Report
Mon 5 Oct 2015
MT.R1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMT.R1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10691656
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTTA49700.024.6766411
GGTTAAT45600.024.3867443
GCTAGTG5200.024.20740792-93
TGGTTAA48800.022.3994452
GTTAATA55700.020.4764584
CGCTAGT5950.019.958490-91
TAGACTC44200.017.4083619
AATAGAC45100.016.8504147
ACGCTAG7700.016.65619390-91
TGACTCG33650.016.6557468
CCTAGCG27050.016.15519136-37
TTAGGGC17200.014.9208832
CTTAGGG17000.014.8200511
CTTAGAA56100.014.3201181
GACGCTA9150.014.27632188-89
TTGACTC40250.014.1606287
GCCCTAG31400.014.06807534-35
GACTCTA32800.013.68431610-11
TTAATAC84500.013.5468925
CCCTAGC27800.013.15641336-37
Produced by FastQC (version 0.11.3)