FastQCFastQC Report
Mon 5 Oct 2015
MG.SE.trimmed.hg38.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG.SE.trimmed.hg38.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2828390
Sequences flagged as poor quality0
Sequence length40-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAG1600.0111.7687490-91
CTAGTGC1450.065.3301992-93
CGCTAGT1550.058.376890-91
GCTAGTG1800.052.62710692-93
GACGCTA3500.026.41567888-89
TAGTGCG3501.2732926E-1124.99620294-95
TAGATCG3956.184564E-1122.14853394-95
CACGTGT3250.019.3755536
TTAGATC2250.001132957417.94599294-95
TCACGTG3700.017.870025
CTCAGAC4805.456968E-1217.39352684-85
TAGTACG4600.017.15502572-73
ACGTCTA4006.548362E-1116.61406376-77
TAGCGAC3501.8189894E-1216.2004358-59
TACGTCT4803.6379788E-1215.69105976-77
TAGTACT3700.015.12604246-47
TCAGTAG4501.0913936E-1114.7576968-69
CACGTCA4750.013.9198061
GTAGTAC5457.2759576E-1213.71739472-73
ACGTGTA5100.013.5818827
Produced by FastQC (version 0.11.3)