Paper: Development of common leaf-footed bug pests depends on the presence and identity of their environmentally-acquired symbionts 

Authors: Martha S. Hunter, Edwin Umanzor, Suzanne E. Kelly, Shaira Whittaker, Alison Ravenscraft

Description: This README file describes the data package accompanying the above publication.

Files in Hunter2021_Data:

1. Hunter2021_phyllopus_devtimes.csv: Development times of L. phyllopus subjected to five treatments: aposymbiotic, or colonized by one of four different Burkholderia isolates. Every row represents one nymph.
	▪	boxid: The cage in which a nymph was reared.
	▪	strain: Identity of the isolate a nymph was infected with. symA = Lep1A1, symT = TF1N1, symP = Lep1P3, symS = SL2Y3, apo = aposymbiotic.
	▪	startdate: Date that a nymph began the first instar (i.e. hatched from its egg)
	▪	start2nd: Date that a nymph began the 2nd instar. NA if nymph died before reaching 2nd instar.
	▪	start3rd: Date that a nymph began the 3rd instar. NA if nymph died before reaching 3rd instar.
	▪	start4th: Date that a nymph began the 4th instar. NA if nymph died before reaching 4th instar.
	▪	start5th: Date that a nymph began the 5th instar.  NA if nymph died before reaching 5th instar.
	▪	adultdate: Date that a nymph eclosed as an adult.  NA if nymph died before reaching adulthood.
	▪	secdays: Development time from hatch to the beginning of the 2nd instar in days. NA if nymph died before reaching 2nd instar.
	▪	thirdays: Development time from hatch to the beginning of the 3rd instar in days. NA if nymph died before reaching 3rd instar.
	▪	fourthdays: Development time from hatch to the beginning of the 4th instar in days. NA if nymph died before reaching 4th instar.
	▪	fifthdays: Development time from hatch to the beginning of the 5th instar in days.   NA if nymph died before reaching 5th instar.
	▪	dev1toadult: Duration of development from hatch to adulthood in days. NA if nymph died before reaching adulthood.
	▪	dev2toadult: Duration of development from the beginning of the 2nd instar to adulthood in days. NA if nymph died before reaching adulthood.
	▪	deathstage: The instar in which a nymph died. Nymphs that successfully reached adulthood have an NA in this column.
	▪	deathdate: The date on which a nymph died. Nymphs that successfully reached adulthood have an NA in this column.
	▪	apo.sym: Indicates whether a nymph was symbiotic (infected with Burkholderia of any strain) or aposymbiotc.
	▪	notes: Any notes about the individual. NA if there were no notes.

2. Hunter2021_phyllopus_masses.csv: Fresh masses at adulthood of L. phyllopus subjected to five treatments: aposymbiotic, or colonized one of four different Burkholderia isolates.
	▪	date: Date on which mass was measured.
	▪	boxid: As above.
	▪	bugid: ID number of the individual bug.
	▪	sex: male (M) or female (F)
	▪	mass: Fresh weight in mg upon eclosion to adulthood.
	▪	owner: Name of the student who reared the insect and collected the mass data.
	▪	strain: As above.
	▪	apo.sym: As above.
	▪	sex.as: Combination of the apo.sym and sex columns.
	▪	notes: Any notes about the individual. NA if there were no notes.

3. Hunter2021_phyllopus_pcr_results.csv: Results of diagnostic PCR on L. phyllopus DNA extracts to check the Burkholderia infection status in the experimental insects that reached adulthood.
	▪	bugid: As above.
	▪	pcr.result: “pos” indicates that the insect was infected with Burkholderia, as evidenced by a band on the gel. “neg” indicates insects in which Burkholderia DNA was undetectable - no band was visible on the gel. NA indicates that no PCR test result was available, due for example to a failed DNA extraction.
	▪	strain: A = Lep1A1, T = TF1N1, P = Lep1P3, S = SL2Y3, apo = aposymbiotic.
	▪	mismatch: “yes” indicates a mismatch between the assigned treatment and the PCR result. “no” indicates that the PCR result and the assigned treatment did match. Individuals with a “yes” were thrown out prior to analysis.
	▪	notes: Any notes about the individual. NA if there were no notes.

4. Hunter2021_zonatus_devtimes_4isolates.csv: Development times of L. zonatus colonized by one of four different Burkholderia isolates. Every row represents one nymph.
	▪	boxid: As above.
	▪	strain: Identity of the isolate a nymph was infected with. 
	▪	startdate: Date that a nymph began the 2nd instar.
	▪	adultdate: Date that a nymph eclosed to adulthood. (Data from nymphs that died during development is not included here.)
	▪	devtime: Duration of development from the beginning of the 2nd instar to adulthood in days.

5. Hunter2021_zonatus_devtimes_aposym.csv: Development times of L. zonatus that either were, or were not, colonized by Burkholderia. Every row represents one nymph. Symbiotic nymphs were all infected with the Lep1A1 isolate.
	▪	boxid: As above.
	▪	rep: The ID number of an individual nymph within a given cage.
	▪	trt: “apo” = aposymbiotic (not exposed to Burkholderia), “sym” = symbiotic (exposed to Lep1A1 during the 2nd instar).
	▪	secdays, thirdays, fourthdays, fifthdays: As above.
	▪	adultdays: Duration of development from hatch to adulthood in days.  NA if nymph died before reaching adulthood.
	▪	stagedeath: The instar in which a nymph died. 6 indicates that the nymph successfully reached adulthood.
	▪	sex: As above. NA if sex could not be determined. (Sex cannot be determined for nymphs that failed to reach adulthood.)
	▪	weight: Fresh weight in mg upon eclosion to adulthood.  NA if nymph died before reaching adulthood.
	▪	notes: Any notes about the individual. NA if there were no notes.

6. Hunter2021_zonatus_masses.csv: Fresh masses at adulthood of L. zonatus colonized by one of four different Burkholderia isolates. Every row represents one nymph.
	▪	boxid: As above.
	▪	sampleid: ID number of the individual bug.
	▪	strain: Identity of the isolate a nymph was infected with. 
	▪	mass: Fresh weight in mg upon eclosion to adulthood.  NA if nymph died before reaching adulthood.
	▪	sex: “f” = female, “m” = male.   NA if nymph died before reaching adulthood. (Nymphs that did not reach adulthood could not be sexed.)
	▪	deathinstar: Instar in which a nymph died. “Ad” indicates individuals that successfully reached adulthood.
	▪	startdate: As above.
	▪	deathdate: The date on which a nymph died. Nymphs that successfully reached adulthood have an NA in this column.
	▪	notes: Any notes about the individual. NA if there were no notes.

7. Hunter2021Supplement_phylogeny_metadata.csv: Metadata for the tips of the Burkholderia 16S phylogeny in Figure S1. Focal isolates were Sanger-sequenced for this study.
	▪	accession: Accession number.
	▪	tipname: The tip label used on the phylogeny. The genus of all Burkholderia sensu lato is assigned as Burkholderia.
	▪	newname: Same as above, except genera of Burkholderia s.l. have been converted to the new genus names Burkholderia (sensu stricto), Paraburkholderia, and Caballeronia.
	▪	description: The sequence description as it appears on NCBI Genbank.
	▪	type: “origiso” indicates the four focal isolates. “refseq’ indicates a sequence downloaded from Genbank.
	▪	clade: Indicates membership of a Burkholderia s. l. species in one of the named clades.
	▪	genome.position: The location of the extracted 16S sequence for complete genome sequences that were downloaded from GenBank. NA for all other sequences.