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Measure intensity along path

Description

Measures the intensity profile along the pixel-wide backbone of an object and outputs this profile to .xlsx file. Input objects are skeletonised to single pixel-wide representations prior to measurement; however, pre-skeletonised objects can also be processed.

Output results are stored in a multi-sheet .xlsx file, where each sheet includes the profile for a specific input image. Each row of a sheet contains the profile for a single object. Profiles are linearly-interpolated such that each measured position along a profile is 1px from the previous.

Note: Objects must either form a single line (i.e. not contain multiple branches) or reduce to a single line during skeletonisation. No profile will be recorded for any objects which fail this requirement.

Parameters