The dataset contains the biological triplicate mass spectrometry runs from the following sample: - doublet microtubles from Tetrahymena thermophila SB255 cells - doublet microtubles treated with NaCl from Tetrahymena thermophila SB255 cells - doublet microtubles from Tetrahymena thermophila K40R mutant (Lysine 40 of alpha tubulin mutated to Arg) - doublet microtubles from Tetrahymena thermophila Mec17 mutants (Knockout of Mec17 gene) The K40R & Mec17 mutants are described in https://doi.org/10.1038/nature09324 The samples are viewable using Scaffold software.