Raw data for the article: Diez Rodriguez, B.; Kloth, K. & Albrectsen, B.R. (2021). The effects of condensed tannins on behaviour and performance of a specialist aphid on Aspen ----------------------------- The data is composed of eight datasets: ----------------------------------------- * Aphid_fitness.csv * Aphid_development.csv * Aphid_life_tables.csv * Tannin_concentrations.csv * Tannin_induction.csv * Tannin_seasonal_changes.csv * Aspen_lines_timebins_0-8h.csv * Aspen_lines_variables_0-8h_plsda.csv Composition of the aphid fitness dataset ------------------------------------------------------------ The dataset contains 9 columns * This dataset contains mock observations: Mock observations were included for the sake of plotting the boxplots in Figure 2. - plantID [Integer]: a given ID for each plant individual in the dataset. Mock observations are preceded with the character "m" - env [String]: experimental setting for each observation, field or lab (greenhouse) - GT [Integer]: SwAsp genotype of each individual - aphid_treatment [String]: indicates if the individuals were infested ("infested") with aphids or not ("control") - N_treatment [String]: indicates if the individual was treated with nitrogen (yes) or not ("no") - nymphs [Integer]: number of aphid nymphs (any aphid that was not an adult) - adult_aphids [Integer]: number of adult aphids (adult, wingless females) - total_aphids [Integer]: number of total aphids. the sum between nymphs and adults - value [String]: Indicates if the observation corresponds to a "real" observation or a "mock" observation Composition of the aphid development dataset ------------------------------------------------------------ The dataset contains 7 columns - plantID [Integer]: a given ID for each plant individual in the dataset - GT [Integer]: SwAsp genotype of each individual - age [Integer]: age of the aphid, measured in days. Starts when the nymph was first placed on the new leaf - days_offspring [Integer]: day count of nymph production. Count starts the day in which a new nymph was first observed after placing the original nymph on the new leaf - cum_nymps [Integer]: number of cumulative number of nymphs - plantlet_batch [Integer]: in vitro propagation batch - n_nymphs [Integer]: number of nymphs produced each individual day Composition of the aphid life tables dataset ------------------------------------------------------------ The dataset contains 9 columns - plantID [Integer]: a given ID for each plant individual in the dataset. - GT [Integer]: SwAsp genotype of each individual - plantlet_batch [Integer]: in vitro propagation batch - offspring_female [Float]: number of nymphs produced per adult aphid, calculated as total_#nymphs/3 - nymphs_day [Float]: number of nymphs produced each individual day, calculated as #nymphs/#days - prereproductive_period [Float]: the number of days measured from the day a 24 hour (or less) old nymph was placed on the tree until the day when the nymph reached the reproductive stage and the first new nymph was observed - rm [Float]: intrinsic increase rate, calculated as rm = 0.738 (logeMd)/d, where d is the pre-reproductive period in days, Md is the total number of nymphs - DT [Float]: Doubling time, time required for the population to double in the number of individuals. Calculated as DT = ln(2)/rm - time [String]: Time when the experiment was conducted. Life table parameters were calculated in two separate experiments conducted in "autumn" and "spring". Composition of the tannin concentrations dataset ------------------------------------------------------------ The dataset contains 7 columns - uniqueID [Integer]: a unique ID given to each observation in this dataset - plantID [Integer]: a given ID for each plant individual in the dataset - GT [Integer]: SwAsp genotype of each individual - induction_type [String]: Type of tannin induction. "Control" for measurements taken before aphid infestation, "local" for measurements done in leaves infested directly with aphids and "systemic" for measuremens done in orthostichous leaves - total_aphids [Integer]: number of adult aphids (adult, wingless females) - tannin_concentration [Float]: Foliar tannin concentration given in mg/g DW (dry weigth) - induced_tannins [Float]: Change in foliar tannin concentration, obtained by substracting the concentration before aphid infestation from the concentration after infestation - env [String]: experimental setting for each observation, field or lab (greenhouse) Composition of the tannin induction dataset ------------------------------------------------------------ The dataset contains 6 columns - plantID [String]: a given ID for each plant individual in the dataset - GT [Integer]: SwAsp genotype of each individual - total_aphids [Integer]: number of adult aphids (adult, wingless females) - control_CTs [Float]: tannin concentration before aphid infestation, given in mg/g DW (dry weigth) - local_CTs [Float]: tannin concentration in locally infested leaves , given in mg/g DW (dry weigth) - systemic_CTs [Float]: tannin concentration in orthostichous leaves, given in mg/g DW (dry weigth) Composition of the tannin seasonal changes dataset ------------------------------------------------------------ The dataset contains 4 columns * This dataset contains only the mean values of all individuals selected for foliar tannin concentration seasonal changes studies under common garden conditions - GT [Integer]: SwAsp genotype - month [String]: Month when the leaves were sample for tannin assays - N_treatment [String]: indicates if the individual was treated with nitrogen (yes) or not ("no") - tannin_concentration [Float]: Foliar tannin concentration given in mg/g DW (dry weigth) Composition of the aspen lines timebins dataset ------------------------------------------------------------ The dataset contains 8 columns - id_rec [Integer]: Unique ID for each EPG recording - group [String]:SwAsp genotype ID of each EPG recording - hour [Integer]: Hour of the recording - wave [Integer]: Type of wave annotated according to the EPG Systems software. For more info, see: https://www.epgsystems.eu/downloads-install-files-manuals/file/137-last-update-2-oct-20 - wavename [String]: Name of the wave according to the EPG Systems annotation for aphid feeding behaviours - perc [Float]: Percentage of time spent of various aphid feeding activities - num [Integer]: Number of events - mean [Float]: Mean percentage of time per event Composition of the aspen lines PLSD dataset ------------------------------------------------------------ The dataset contains 19 columns * Variables correspond to annotated EPG feeding behaviour activities. , C = pathway, Pd = potential drops, RPD (w11) = repetitive potential drops, E1 = salivation in phloem, E2 = phloem ingestion, F = penetration difficulties in the cell wall, G = xylem ingestion - tannin [String]: Tannin group, can be high (H) or low (L) - id_rec [Integer]: Unique ID for each EPG recording - group [String]: SwAsp genotype ID of each EPG recording - sum.np [Float]: Total duration no probing - sum.c [Float]: Total duration C - mean.c [Float]: Mean duration C - rate.pd [Float]: Rate of Pd - lat.e1 [Float]: Latency to first E1 - sum.e1 [Float]: Total duration E1 - mean.e1 [Float]: Mean duration E1 - max.e1 [Float]: Max duration E1 - sum.e2 [Float]: Total duration E2 - sum.e2s [Float]: Total duration of E2 events > 10 min - mean.e2 [Float]: Mean duration E2 - max.e2 [Float]: Max duration E2 - sum.f [Float]: Total duration F - sum.g [Float]: Total duration G - sum.w11 [Float]: Total duration RPD - mean.w11 [Float]: Mean duration RPD