In a nutshell

  1. Select your rank of interest (e.g. Family)
  2. Select a statistical test and press "Select Mode"
  3. Press "Do Stats"
  4. Click on "P" in the table to sort the bacterial groups by p-value.
  5. The p-value distribution (histogram) on the left side of the figure indicates if low (significant) p-values are relevant or observed poorly by chance. A uniform p-value distribution indicates that low p-values were observed by chance. The uniform (expected) p-value distribution is indicated by the red line "Expected". An increase of low p-values (a negative binominal distribution with small r) indicates that low p-values are relevant (significant) and that the compared groups are different.