In a nutshell
- Select the format of your file, choose your file, press Convert and save the converted
file locally on your computer by clicking with the right mouse button on "Converted File"
and choosing "Save file as".
- To convert QIIME files, first convert the QIIME mapping file. Set format to
"QIIME Mapping", choose the mapping file, press Convert and save the file locally on
your computer. Then, convert the QIIME taxonomic assignments (obtained by
assign_taxonomy.pl) to the Calypso format. Finally, convert the OTU table.
- Open/import the converted Calypso meta data file in Excel and set the
group, individual, sample time/location of each sample. For example, if the
samples were taken from obese and lean mice, the groups would be "lean" and "obese",
the individuals would be the names/ids of each animal. Sample time or location could
for example be "T1, T2, caecum, large intestine, small intestine, feces". Save the
file in comma separated format. Open the saved file in notepad or Wordpad to control
the file format.