In a nutshell

  1. Select a rank (e.g. genus or OTU) and press "Draw Chart".
  2. To obtain high-quality figures, adjust the image resolution, width and height.
  3. Increasing curves indicate an under-sampling, i.e. that not all bacterial groups of the underlying community are represented by the sequence data. Reads are randomly selected from each sample and the number of present taxa/OTUs counted. Mean species richness is the number of taxa/OTUs observed for a given sample size.