ThunderSTORM (1.3-2014-11-08)

Input:
{
  "title": "201707149_NucEnv_glass_125mMMEApH79_14_80mmlens_150mW_5msexp.tif"
}
Camera settings:
{
  "isEmGain": true,
  "pixelSize": 102.0,
  "offset": 100.0,
  "photons2ADU": 15.01,
  "gain": 100.0
}
Analysis:
{
  "name": "Wavelet filter (B-Spline)",
  "parameters": {
    "prefsPrefix": "thunderstorm.filters.wave",
    "trackedParameters": {
      "scale": {
        "defaultValue": 2.0,
        "value": 2.0
      },
      "order": {
        "defaultValue": 3,
        "value": 3
      }
    }
  }
}
{
  "name": "Local maximum",
  "connectivity": 8,
  "threshold": "2*std(Wave.F1)",
  "parameters": {
    "prefsPrefix": "thunderstorm.detectors.locmax",
    "trackedParameters": {
      "connectivity": {
        "defaultValue": "8-neighbourhood",
        "value": "8-neighbourhood"
      },
      "threshold": {
        "defaultValue": "std(Wave.F1)",
        "value": "2*std(Wave.F1)"
      }
    }
  }
}
{
  "name": "PSF: Integrated Gaussian",
  "crowdedField": {
    "params": {
      "prefsPrefix": "thunderstorm.estimators.dense.mfa",
      "trackedParameters": {
        "fixed_intensity": {
          "defaultValue": false,
          "value": false
        },
        "expected_intensity": {
          "defaultValue": "500:2500",
          "value": "500:2500"
        },
        "nmax": {
          "defaultValue": 5,
          "value": 5
        },
        "pvalue": {
          "defaultValue": 1.0E-6,
          "value": 1.0E-6
        },
        "mfaenabled": {
          "defaultValue": false,
          "value": false
        },
        "keep_same_intensity": {
          "defaultValue": true,
          "value": true
        }
      }
    },
    "name": "Multi-emitter fitting analysis"
  },
  "parameters": {
    "prefsPrefix": "thunderstorm.estimators",
    "trackedParameters": {
      "sigma": {
        "defaultValue": 1.6,
        "value": 1.6
      },
      "method": {
        "defaultValue": "Weighted Least squares",
        "value": "Weighted Least squares"
      },
      "full_image_fitting": {
        "defaultValue": false,
        "value": false
      },
      "fitradius": {
        "defaultValue": 3,
        "value": 3
      }
    }
  }
}
Post-processing:
[
  {
    "options": "magnification=5.0 save=false showcorrelations=false method=[Cross correlation] steps=15"
  },
  {
    "options": "formula=[(uncertainty > 3 & uncertainty < 30)]"
  },
  {
    "options": "offframes=5 framespermolecule=0 zcoordweight=0.1 dist=20.0"
  },
  {
    "options": "neighbors=5 dimensions=2D radius=50.0"
  }
]