DNA alignment for the genus Leucadendron phylogeny
This alignment is the concatenation of nine markers that were used to reconstruct the phylogeny of the genus Leucadendron in the paper cited above. Those markers were amplified for 81 Leucadendron taxa collected in natural populations in the South-African fynbos, except for three external groups which were collected in the Kirstenbosch Botanical Garden: Paranomus spathulatus, Mimetes cucullatus and Leucospermum erubescens. A detailed map of the alignment is given below: \nPositions 1 - 618 : AS1 marker which contains an exon of 618bp. \nPositions 619 - 1046 : PPR-like marker which contains an exon of 233bp and a 3'-UTR of 195bp. \nPositions 1047 - 1800 : SVR7 marker which contains an exon of 652bp and a 3'-UTR of 102bp. \nPositions 1801 - 2181 : CAF1-6 marker which only contains an exon of 381bp. \nPositions 2182 - 2795 : the classical ITS marker. \nPositions 2796 - 3591 : ATINT1 marker which contains an exon of 665bp and a 3'-UTR of 131bp. \nPositions 3592 - 3868 : APO2 marker which contains only an exon of 277bp. \nPositions 3869 - 4207 : APG6 marker which contains only an exon of 339bp.\nPositions 3208 - 4865 : ATPHAN marker which contains only an exon of 658bp\nThe methodology used to design the following markers: PPR-like, CAF1-6, ATINT1, APO2, APG6 and ATPHAN is described in (Tonnabel et al. 2013). The AS1 marker was designed in (Illing et al. 2009). \n\nTonnabel, J., Olivieri, I., Mignot, A., Rebelo, A., Justy, F., Santoni, S., Caroli, S., Saun\u00e9, L., Bouchez, O. & Douzery, E.J.P. (2014) Developing nuclear DNA phylogenetic markers in the angiosperm genus Leucadendron (Proteaceae): a next-generation sequencing transcriptomic approach, Molecular Phylogenetics and Evolution, 70, 37-46.\nIlling, N., Klak, C., Johnson, C., Brito, D., Negrao, N., Baine, F., van Kets, V., Ramchurn K.R., Seoighe, C. & Roden, L. (2009) Duplication of the Asymmetric Leaves1/Rough Sheath 2/Phantastica (ARP) gene precedes the explosive radiation of the Ruschioideae. Development genes and evolution, 219, 331-338.
Tonnabeletal_Evolution_alignment_Leucadendron.fas
data_sexspecific_leaf_measurements
This dataset corresponds to the sex-specific leaves measurements realized for the Leucadendron genus (L.) in order to assess sexual dimorphism at the leave level. Leaf area (named area), leaf length (named length) and leaf width (named width) have been assessed for several individuals (ind) of several populations (pop) of different species (species). Within the file, females are designed by 'F' and males by 'M'.
data_sexspecific_leaf_measurement.ods
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