ReadMe file for: "Doumayrou et al_evolution_traits_data.xls"

Introduction:

-This data file contains life history data (i.e. virulence, transmission rate and within-host accumulation) of four natural isolates of Cauliflower mosaic virus infecting Brassica rapa to test the evolution of the virulence, transmission and viral accumulation of isolates with low and high within-host accumulation discussed in the manuscript.
- Data for Figures 4, S3, S4 and Table 1
-This file contains two worksheets. The first lists all the life history variables for each individually infected plant used in the published study. The second worksheet lists the transmission data which were used for the GLM analysis.
-All data were collected by J. Doumayrou (contact: Juliette.doumayrou@gmail.com)

Description:

*Column, Headings and description

First worksheet named “VTA_time”
-A. Isolate/healthy plant: name of the inoculated isolate or control (=“healthy plant”).
-B. replicate: replicate number.
-C. time: time after inoculation which traits were measured (days post-inoculation, dpi)
-D.accumulation_group : “low” corresponds plant infected by isolates accumulating at low levels 
and “high” isolates accumulating at high levels. "-" corresponds to control.
-E. log10_accumulation: logarithmic transformation applied to NRQ data i.e. virus load relative to the actin load in each infected plant according to equation (1) in the manuscript.
-F.#_infected_plants: number of recipient infected plants after inoculation by aphids for each donor plant.
-G. #_noninfected_plants: number of recipient noninfected plants after inoculation by aphids for each donor plant.
-H. total_plants:  number of total recipient plants exposed to aphids for each donor plant.
-I. leaf_area_0dpi: leaf area of each donor plant at 0 dpi (cm²) used for each obtervation time.
-J.  leaf_area: leaf area of each donor plant at different observation time after inoculation (cm²).
-K. relative_leaf_area: leaf area of each donor plant at different observation time relative to healthy plants at the same time according to equation (2) in the manuscript.


Second worksheet named “Transmission_binomial_time”

-A. Isolate: name of the inoculated isolate.
-B. time: time after inoculation which traits were measured (dpi)
-C. donor_plant: number of donor plants for each isolate.
-D. recipieny_plant: number of recipient infected plants after inoculation by aphids for each donor plant and each isolate.
-E. infnoninf: for each recipient plant, “1” corresponds to an infected plant and “0” to a non-infected plant exposed to aphids at different times after inoculation.

*Missing data:
-Cells with missing data are indicated as “na”