This file describes all supplementary material provided alongside: Tandem-Running and Scouting Behavior is Characterized by Up-Regulation of Learning and Memory formation genes within the Ant Brain (A. Alleman, M. Stoldt, B. Feldmeyer, S. Foitzik) 2019 File: Table_S1.xlsx Description: Expression matrix T. americanus Software used: RSEM v.1.3.0 Contig = Transcript in the T. americanus transcriptome A-114-Fo-Tam = Read counts of the follower sample from colony A-114 in T. americanus A-114-Le-1-Tam = Read counts of the leader sample from colony A-114 in T. americanus D-80-Sc-1-Tam = Read counts of the scout sample from colony D-80 in T. americanus F-410-Sc-1-Tam = Read counts of the scout sample from colony F-410 in T. americanus H-209-Le-1-Tam = Read counts of the leader sample from colony H-209 in T. americanus H-232-Fo1-Tam = Read counts of the follower sample from colony H-232 in T. americanus H-232-Le-1-Tam = Read counts of the leader sample from colony H-232 in T. americanus M-517-Sc-1-Tam = Read counts of the scout sample from colony M-517 in T. americanus O-520-Fo-2-Tam = Read counts of the follower sample from colony O-520 in T. americanus O-520-Le-2-Tam = Read counts of the leader sample from colony O-520 in T. americanus O-520-Sc-1-Tam = Read counts of the scout sample from colony O-520 in T. americanus O-551-Fo1-Tam = Read counts of the follower sample from colony O-551 in T. americanus O-551-Le-2-Tam = Read counts of the leader sample from colony O-551 in T. americanus T-891-Fo-1-Tam = Read counts of the follower sample from colony T-891 in T. americanus T-891-Sc1-Tam = Read counts of the scout sample from colony T-891 in T. americanus File: Table_S2.xlsx Description: Expression matrix T. longispinosus Software used: RSEM v.1.3.0 Contig = Transcript in the T. longispinosus transcriptome A-941-Fo-1-TL = Read counts of the follower sample from colony A-941 in T. longispinosus A-941-Le-2-TL = Read counts of the leader sample from colony A-941 in T. longispinosus A-941-Sc-2-TL = Read counts of the scout sample from colony A-941 in T. longispinosus F-381-Fo-2-TL = Read counts of the follower sample from colony F-381 in T. longispinosus F-381-Le-1-TL = Read counts of the leader sample from colony F-381 in T. longispinosus F-381-Sc-1-TL = Read counts of the scout sample from colony F-381 in T. longispinosus J-272-Fo-2TL = Read counts of the follower sample from colony J-272 in T. longispinosus J-272-Le-1-TL = Read counts of the leader sample from colony J-272 in T. longispinosus J-272-Sc-2-TL = Read counts of the scout sample from colony J-272 in T. longispinosus O-Fo-1-TL = Read counts of the follower sample from colony O in T. longispinosus O-Le-1-TL = Read counts of the leader sample from colony O in T. longispinosus O-Sc1-TL = Read counts of the scout sample from colony O in T. longispinosus U-663-Fo-2-TL = Read counts of the follower sample from colony U-663 in T. longispinosus U-663-Le-1-TL = Read counts of the leader sample from colony U-663 in T. longispinosus U-663-Sc-1-TL = Read counts of the scout sample from colony U-663 in T. longispinosus File: Table_S3.xlsx Description: Differentially expressed genes of T. americanus Software used: R packages DESeq2 v.1.16.1 -> This table consists of six sub-tables, one for each pairwise comparison between behaviors with only overexpressed contigs with a adjusted p-value<0.05& Dunn p-value<0.05 shown, all containing the same columns ID = Contig ID of the contig overexpressed in this specific pairwise comparison Base Mean = Average of the normalized count values, dividing by sizefactors, taken over all samples Log Fold Change = log2foldchange, the effect size estimate lfcSE = Standard error of the Log Fold Change stat = Wald statistic of the Wald test P-value = The probability to get the same or stronger effect size under the null hypothesis Adjusted p-value = The p-value adjusted for multiple testing using False Discovery Rate (FDR) Kruskal-Wallis p-value = The p-value of the Kruskal-Wallis test comparing the read counts of all three behaviors and whether they can be explained by behavioral group Dunn p-value = The p-value of the pairwise Dunn test performed on the read counts of the comparison already investigated by DESeq2 and adjusted for mulitple testing using Benjamini-Hochberg procedure BLAST Hit = The BLAST Hit with the best score and an E-value of less than 1e-05 against the invertebrate protein database Read count Follower 1 = Read counts of follower sample 1 in T. americanus Read count Follower 2 = Read counts of follower sample 2 in T. americanus Read count Follower 3 = Read counts of follower sample 3 in T. americanus Read count Follower 4 = Read counts of follower sample 4 in T. americanus Read count Follower 5 = Read counts of follower sample 5 in T. americanus Read count Leader 1 = Read counts of leader sample 1 in T. americanus Read count Leader 2 = Read counts of leader sample 2 in T. americanus Read count Leader 3 = Read counts of leader sample 3 in T. americanus Read count Leader 4 = Read counts of leader sample 4 in T. americanus Read count Leader 5 = Read counts of leader sample 5 in T. americanus Read count Scout 1 = Read counts of scout sample 1 in T. americanus Read count Scout 2 = Read counts of scout sample 2 in T. americanus Read count Scout 3 = Read counts of scout sample 3 in T. americanus Read count Scout 4 = Read counts of scout sample 4 in T. americanus Read count Scout 5 = Read counts of scout sample 5 in T. americanus File: Table_S4.xlsx Description: Differentially expressed genes of T. longispinosus Software used: R packages DESeq2 v.1.16.1 -> This table consists of six sub-tables, one for each pairwise comparison between behaviors with only overexpressed contigs with a adjusted p-value<0.05& Dunn p-value<0.05 shown, all containing the same columns ID = Contig ID of the contig overexpressed in this specific pairwise comparison Base Mean = Average of the normalized count values, dividing by sizefactors, taken over all samples Log Fold Change = log2foldchange, the effect size estimate lfcSE = Standard error of the Log Fold Change stat = Wald statistic of the Wald test P-value = The probability to get the same or stronger effect size under the null hypothesis Adjusted p-value = The p-value adjusted for multiple testing using False Discovery Rate (FDR) Kruskal-Wallis p-value = The p-value of the Kruskal-Wallis test comparing the read counts of all three behaviors and whether they can be explained by behavioral group Dunn p-value = The p-value of the pairwise Dunn test performed on the read counts of the comparison already investigated by DESeq2 and adjusted for mulitple testing using Benjamini-Hochberg procedure BLAST Hit = The BLAST Hit with the best score and an E-value of less than 1e-05 against the invertebrate protein database Read count Follower 1 = Read counts of follower sample 1 in T. longispinosus Read count Follower 2 = Read counts of follower sample 2 in T. longispinosus Read count Follower 3 = Read counts of follower sample 3 in T. longispinosus Read count Follower 4 = Read counts of follower sample 4 in T. longispinosus Read count Follower 5 = Read counts of follower sample 5 in T. longispinosus Read count Leader 1 = Read counts of leader sample 1 in T. longispinosus Read count Leader 2 = Read counts of leader sample 2 in T. longispinosus Read count Leader 3 = Read counts of leader sample 3 in T. longispinosus Read count Leader 4 = Read counts of leader sample 4 in T. longispinosus Read count Leader 5 = Read counts of leader sample 5 in T. longispinosus Read count Scout 1 = Read counts of scout sample 1 in T. longispinosus Read count Scout 2 = Read counts of scout sample 2 in T. longispinosus Read count Scout 3 = Read counts of scout sample 3 in T. longispinosus Read count Scout 4 = Read counts of scout sample 4 in T. longispinosus Read count Scout 5 = Read counts of scout sample 5 in T. longispinosus File: Table_S5.xslx Description: Contigs in significant modules of WGCNA in T. americanus Software used: R packages WGCNA v.1.64-1, ggpubr v.0.1.8, DESeq2 v.1.16.1 Module 145 = Contig IDs of contigs belonging to the module 145 in T. americanus Module 179 = Contig IDs of contigs belonging to the module 179 in T. americanus Module 254 = Contig IDs of contigs belonging to the module 254 in T. americanus Module 260 = Contig IDs of contigs belonging to the module 260 in T. americanus Module 266 = Contig IDs of contigs belonging to the module 266 in T. americanus Module 276 = Contig IDs of contigs belonging to the module 276 in T. americanus File: Table_S6.xslx Description: Contigs in significant modules of WGCNA in T. longispinosus Software used: R packages WGCNA v.1.64-1, ggpubr v.0.1.8, DESeq2 v.1.16.1 Module 172 = Contig IDs of contigs belonging to the module 172 in T. longispinosus Module 177 = Contig IDs of contigs belonging to the module 177 in T. longispinosus Module 203 = Contig IDs of contigs belonging to the module 203 in T. longispinosus Module 208 = Contig IDs of contigs belonging to the module 208 in T. longispinosus File: Table_S7.xlsx Description: Enriched GO terms in DEGs and modules of T. americanus Software used: InterProScan v.5.25-64.0, R package topGO v.2.28.0 Gene List = List of differentially expressed contigs/ contigs in modules of WGCNA whose annotations are compared to the ones of the filtered transcriptome of T. americanus GO ID = ID of the enriched GO term Term = Name of the enriched GO term Annotated = Number of contigs annotated with this GO term in the filtered transcriptome of T. americanus Significant = Number of contigs annotated with this GO term in the gene list Expected = Number of contigs to be expected to be annotated concerning the number of annotations in the transcriptome and the size of the gene list Fisher = P-value of the Fishers exact test File: Table_S8.xlsx Description: Enriched GO terms in DEGs and modules of T. longispinosus Software used: InterProScan v.5.25-64.0, R package topGO v.2.28.0 Gene List = List of differentially expressed contigs/ contigs in modules of WGCNA whose annotations are compared to the ones of the filtered transcriptome of T. longispinosus GO ID = ID of the enriched GO term Term = Name of the enriched GO term Annotated = Number of contigs annotated with this GO term in the filtered transcriptome of T. longispinosus Significant = Number of contigs annotated with this GO term in the gene list Expected = Number of contigs to be expected to be annotated concerning the number of annotations in the transcriptome and the size of the gene list Fisher = P-value of the Fishers exact test File: Table_S9.xlsx Description: Enriched KEGG pathways in DEGs and modules of T. americanus Software used: InterProScan v.5.25-64.0 Gene Set = List of differentially expressed contigs/ contigs in modules of WGCNA whose pathway are compared to the ones of the filtered transcriptome of T. americanus Pathway = Name of the enriched pathway Annotated = Number of contigs annotated with this pathway in the filtered transcriptome of T. americanus Not-Annotated = Number of contigs not annotated with this pathway in the filtered transcriptome of T. americanus Significant = Number of contigs annotated with this pathway in the gene list Not-Significant = Number of contigs not annotated with this pathway in the gene list P-value = P-value of hypergeometric test adjusted p-value = P-value adjusted for multiple testing using Benjamini-Hochberg procedure File: Table_S10.xlsx Description: Enriched KEGG pathways in DEGs and modules of T. longispinosus Software used: InterProScan v.5.25-64.0 Gene Set = List of differentially expressed contigs/ contigs in modules of WGCNA whose pathway are compared to the ones of the filtered transcriptome of T. longispinosus Pathway = Name of the enriched pathway Annotated = Number of contigs annotated with this pathway in the filtered transcriptome of T. longispinosus Not-Annotated = Number of contigs not annotated with this pathway in the filtered transcriptome of T. longispinosus Significant = Number of contigs annotated with this pathway in the gene list Not-Significant = Number of contigs not annotated with this pathway in the gene list P-value = P-value of hypergeometric test adjusted p-value = P-value adjusted for multiple testing using Benjamini-Hochberg procedure File: Table_S11.xslx Description: Orthogroups found between transcriptomes of T. americanus and T. longispinosus Software used: Orthofinder v.1.1.8 Name of Orthogroup = Name of the single-copy orthogroup to which the contigs named in the following two columns belong Orthologue in T. americanus = Contig ID of the orthologue in the transcriptome of T. americanus Orthologue in T. longispinosus = Contig ID of the orthologue in the transcriptome of T. longispinosus File: Table_S12.xlsx Description: Orthogroups found between DEGs of T. americanus and T. longispinosus Software used: Orthofinder v.1.1.8 Name of Orthogroup = Name of the single-copy orthogroup to which the contigs named in the following two columns belong Orthologue in DEGs of T. americanus = Contig ID of the orthologue in the list of differentially expressed contigs in T. americanus Orthologue in T. longispinosus = Contig ID of the orthologue in the transcriptome of T. longispinosus File: Table_S13.xlsx Description: Orthogroups found between DEGs of T. longispinosus and T. americanus Software used: Orthofinder v.1.1.8 Name of Orthogroup = Name of the single-copy orthogroup to which the contigs named in the following two columns belong Orthologue in DEGs of T. longispinosus = Contig ID of the orthologue in the list of differentially expressed contigs in T. longispinosus Orthologue in T. americanus = Contig ID of the orthologue in the transcriptome of T. americanus File: Script S1.r Description: R script for the gene expression analysis using DESeq2 in T. americanus Software used: R packages DESeq2 v.1.16.1, VennDiagram v.1.6.17, ggplot2 v.3.0.0 File: Script_S2.r Description: R script for the gene expression analysis using DESeq2 in T. longispinosus Software used: R packages DESeq2 v.1.16.1, VennDiagram v.1.6.17, ggplot2 v.3.0.0 File: Script_S3.r Description: R script for the GO enrichment analysis using TopGO in T. americanus Software used: R packages topGO v.v.2.28.0, evaluate v.0.11 File: Script_S4.r Description: R script for the GO enrichment analysis using TopGO in T. longispinosus Software used: R packages topGO v.v.2.28.0, evaluate v.0.11 File: Script_S5.r Description: R script for the Weighted Gene Co-expression Network Analysis using the R package WGCNA in T. americanus Software used: R packages WGCNA v.1.64-1, ggpubr v.0.1.8, DESeq2 v.1.16.1 File: Script_S6.r Description: R script for the Weighted Gene Co-expression Network Analysis using the R package WGCNA in T. longispinosus Software used: R packages WGCNA v.1.64-1, ggpubr v.0.1.8, DESeq2 v.1.16.1 File: Script_S7.r Description: R script to obtain single-copy orthogroups from Orthofinder output and testing for expression pattern similarities between orthologues of DEGs found in one species in the transcriptome of the other species and vice versa Software used: R packages car v.3.0-2, lme4 v.1.1-18-1, ggplot2 v.3.0.0, MASS v.7.3-50, multcomp v.1.4-8, plyr v.1.8.4, VennDiagram v.1.6.17 File: Tamer_BLAST_transcriptome.tab Description: BLAST annotations of all contigs in the Trinity transcriptome of T. americanus Software used: blastx in NCBI BLAST v2.6.0 For a more precise explanation of the variables please see: https://www.ncbi.nlm.nih.gov/books/NBK62051/ Read = Contig ID of the annotated transcript in T. americanus Alignment Title = Annotation of the BLAST Hit with the species in [] Score = Score of the alignment = sum of identity, substitution and gap scores Bits = Bit score of the alignment eValue = The number of expected alignments with a similar or better score to be found in a database of equal size Identity+ = The pecentage of bases of the alignments where the bases of the two aligned sequences are actually the same Start = Start position (in bases) of the alignment in the subject sequence Stop = Stop position (in bases) of the alignment in the subject sequence File: Tlongi_BLAST_transcriptome.tab Description: BLAST annotations of all contigs in the Trinity transcriptome of T. longispinosus Software used: blastx in NCBI BLAST v2.6.0 For a more precise explanation of the variables please see: https://www.ncbi.nlm.nih.gov/books/NBK62051/ Read = Contig ID of the annotated transcript in T. longispinosus Alignment Title = Annotation of the BLAST Hit with the species in [] Score = Score of the alignment = sum of identity, substitution and gap scores Bits = Bit score of the alignment eValue = The number of expected alignments with a similar or better score to be found in a database of equal size Identity+ = The pecentage of bases of the alignments where the bases of the two aligned sequences are actually the same Start = Start position (in bases) of the alignment in the subject sequence Stop = Stop position (in bases) of the alignment in the subject sequence File: Tamer_transcriptome_filtered.fasta Description: Transcriptome of T. americanus after all filtering steps prior to gene expression analysis Software used: Trinity v.2.4.0, TransDecoder TransDecoder v.3.0.1, R package DESeq2 v.1.16.1 Details: Sequence file of the transcripts created by Trinity for T. americanus filtered for only those remaining after all filtering steps prior to gene expression analysis. Contig name is given after ">" followed by the nucleotide sequence. File: Tlongi_transcriptome_filtered.fasta Description: Transcriptome of T. longispinosus after all filtering steps prior to gene expression analysis Software used: Trinity v.2.4.0, TransDecoder TransDecoder v.3.0.1, R package DESeq2 v.1.16.1 Details: Sequence file of the transcripts created by Trinity for T. longispinosus filtered for only those remaining after all filtering steps prior to gene expression analysis. Contig name is given after ">" followed by the nucleotide sequence. File: Tamer_Trinity_transcriptome.fasta Description: Transcriptome of T. americanus created by Trinity without any filtering steps Software used: Trinity v.2.4.0 Details: Sequence file of the transcripts created by Trinity for T. americanus. Contig name is given after ">" followed by the nucleotide sequence. File: Tlongi_Trinity_transcriptome.fasta Description: Transcriptome of T. longispinosus created by Trinity without any filtering steps Software used: Trinity v.2.4.0 Details: Sequence file of the transcripts created by Trinity for T. longispinosus. Contig name is given after ">" followed by the nucleotide sequence. File: Tamer_Interproscan_GO.txt Description: Gene ontology annotations of all contigs of the transcriptome of T. americanus Software used: InterProScan v.5.25-64.0 1st Column = Name of the contig of the transcriptome of T. americanus Following Columns = GO IDs of functional annotations (if annotated), separated by "," File: Tlongi_Interproscan_GO.txt Description: Gene ontology annotations of all contigs of the transcriptome of T. longispinosus Software used: InterProScan v.5.25-64.0 1st Column = Name of the contig of the transcriptome of T. longispinosus Following Columns = GO IDs of functional annotations (if annotated), separated by "," File: Tamer_Interproscan_KEGG.txt Description: KEGG pathways of all contigs of the transcriptome of T. americanus Software used: InterProScan v.5.25-64.0 1st Column = Name of the contig of the transcriptome of T. americanus Following Columns = KEGG IDs of pathways (if annotated), separated by ";" File: Tlongi_Interproscan_KEGG.txt Description: KEGG pathways of all contigs of the transcriptome of T. longispinosus Software used: InterProScan v.5.25-64.0 1st Column = Name of the contig of the transcriptome of T. longispinosus Following Columns = KEGG IDs of pathways (if annotated), separated by ";"