Additional Information for Jennings et al., Microsatellite primers for the Pacific Northwest conifer Callitropsis nootkatensis (Cupressaceae). 

There are two files associated with this DRYAD submission:

1. AYC_Population_Info.csv. This comma-delimited file provides location and genotype data for individuals from five populations of
Nootka Cypress (4 Alaskan populations; 1 Oregon population) that were surveyed with 11 microsatellite loci. The file shows individual numbers, 
population names, latitude and longitude (in decimal degrees), and the microsatellite allele length for diploid loci.  Loci that failed to
amplify are indicated by the values "0, 0".

2. AYC_Primer_Info.csv. This comma-delimited file provides information for 144 primers that were screened on five Nootka Cypress populations.
Included are the primer name, forward and reverse primer sequence, the original sequence name from Illumina paired end sequencing, and the 
nucleotide sequence of the microread (2 x 80 bp, with read 1 and read 2 separated by 8 N's).


The original sequences used to construct microsatellite primers were generated on an Illumina GAII using paired 80bp reads. This sequence data
is archived in the EMBL sequence read archive under archive number ERP000647 (http://www.ebi.ac.uk/ena/data/view/ERP000647).

Information on library preparation and data analysis can be found in JENNINGS et al (2011), Multiplexed microsatellite recovery using massively
parallel sequencing. Molecular Ecology Resources, doi: 10.1111/j.1755-0998.2011.03033.x.

Microsatellite fragment sizes were determined on the ABI 3730 Genetic Analyzer (Applied Biosystems, Foster City, CA,
USA) using GeneMapper 4.0 and ROX500 (Applied Biosystems) internal size standards.