T cell repertoire characterization data are provided herein as a supplement to: Defining the alloreactive T cell repertoire using high-throughput sequencing of mixed lymphocyte reaction culture by RO Emerson, JM Mathew, IM Konieczna, HS Robins and JR Leventhal These are the original data used for the analyses presented in the manuscript, 24 files in total: MLR7_TCR1_Fresh_1.adap.txt.results.tsv MLR7_TCR1_Fresh_2.adap.txt.results.tsv MLR7_TCR1_Proliferating_1.adap.txt.results.tsv MLR7_TCR1_Proliferating_2.adap.txt.results.tsv MLR8_TCR2_Fresh_1.adap.txt.results.tsv MLR8_TCR2_Fresh_2.adap.txt.results.tsv MLR8_TCR2_Proliferating_1.adap.txt.results.tsv MLR8_TCR2_Proliferating_2.adap.txt.results.tsv MLR9_TCR3_Fresh_1.adap.txt.results.tsv MLR9_TCR3_Fresh_2.adap.txt.results.tsv MLR9_TCR3_Proliferating_1.adap.txt.results.tsv MLR9_TCR3_Proliferating_2.adap.txt.results.tsv TCR4_Fresh_Responder_R_1.adap.txt.results.tsv TCR4_Fresh_Responder_R_2.adap.txt.results.tsv TCR4_Proliferating_1.adap.txt.results.tsv TCR4_Proliferating_2.adap.txt.results.tsv TCR5_Fresh_Responder_R_1.adap.txt.results.tsv TCR5_Fresh_Responder_R_2.adap.txt.results.tsv TCR5_Proliferating_1.adap.txt.results.tsv TCR5_Proliferating_2.adap.txt.results.tsv TCR6_Fresh_Responder_R_1.adap.txt.results.tsv TCR6_Fresh_Responder_R_2.adap.txt.results.tsv TCR6_Proliferating_1.adap.txt.results.tsv TCR6_Proliferating_2.adap.txt.results.tsv Samples are arranged according to donor pairs; MLR7/TCR4, MLR8/TCR5 and MLR9/TCR6 are the same pairs of subjects, with experiments separated by three months. Each experiment has four associated files, two fresh samples (PCR replicates to increase sampling depth, which were concatenated for the analysis in the paper) and two proliferating samples (the results of two duplicate cell culture experiments). Each file is a set of TCR sequencing data, one line per unique TCR observed in the dataset, with tab-separated columns of additional annotations. Relevant annotation columns: nucleotide - nucleotide sequence of the CDR3 region aminoAcid - Amino Acid translation of the CDR3 region count - Sequence read counts for this receptor frequencyCount - Proportional abundance of this receptor in the dataset cdr3Length - length of the CDR3 region (in NT) vMaxResolved - The V gene segment used in this rearrangement dMaxResolved - The D gene segment used in this rearrangement jMaxResolved - The J gene segment used in this rearrangement vDeletion - The number of bases deleted from the V gene segment during rearrangement d5Deletion - The number of bases deleted from the 5' end of the D gene segment during rearrangement d3Deletion - The number of bases deleted from the 3' end of the D gene segment during rearrangement jDeletion - The number of bases deleted from the J gene segment during rearrangement n2Insertion - The number of inserted bases between the V and DJ gene segments during rearrangement n1Insertion - The number of inserted bases between the D and J gene segments during rearrangement estimatedNumberGenomes - The estimated number of template molecules (i.e., cells) for this receptor in the source sample sequenceStatus - The status (In-frame, out-of-frame, stop codon) of this receptor. 'In' are predicted to encode functional receptor proteins