Colony and individual life-history responses to temperature in a social insect pollinator: Jacob Holland - 06th April 2015 ================================ Hebrew University of Jerusalem -------------------------------- Jacob.Holland@mail.huji.ac.il Associated with: Holland & Bourke 2015; Colony and individual life-history responses to temperature in a social insect pollinator. Functional Ecology. Description: =========== As described in the assoicated paper, these data refer to an experiment where we reared Bombus terrestris (buff-tailed bumble bee) colonies at 20 or 25 degrees celcius and observed life history traits at the colony and individual level. Below is an explanation of all data files. For details of the methods, please see associated research paper (and supplementary materials). Data file details: ================= The data have been recorded separately for the two similar experiments we conducted. This is because the experiments differ in some of the metrics they recorded. Separate data files have been included for each metric, and are all stored in a comma-delimited (.csv) format to preserve data accessability. Please see below for details of each data file. Experiment 1: ~~~~~~~~~~~~ Experiment 1 Colony longevity ----------------------------- Colony = ID code of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. DeathDate = date of colony death (i.e. death of last worker). Given in format ddmmyyyy. DeathDay = same as DeathDate, but expressed in days since the beginning of the experiment. Experiment 1 Colony size ------------------------ Colony = ID code of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. Day = time of census, expressed in days since the beginning of the experiment. Week = same as Day, but expressed in the week bin after the start of experiment. ColSize = the number of workers in the colony assessed by tally counter at census. Given to exact number at start of experiment (day 0 or 1), or where number of workers < 20. Otherwise, rounded to the nearest 5. Experiment 1 Nest temperatures ------------------------------ Colony = ID code of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. Logger = identity of datalogger (B1 or B2) placed in the nest box, contacting brood, used to measure temperature during the measurement session. StartDate = date at the beginning of the measurement session. Given in format ddmmyyyy. StartDay = same as StartDate, but expressed in days since the beginning of the experiment. Tmin = minimum temperature (degrees Celcius) recorded during the session (approx 45h). Tmax = maximum temperature (degrees Celcius) recorded during the session (approx 45h). Tmean = mean temperature (degrees Celcius) recorded during the session (approx 45h). Experiment 1 Queen longevity ---------------------------- Colony = ID code of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. DeathDate = date of colony queen death. Given in format ddmmyyyy. DeathDay = same as DeathDate, but expressed in days since the beginning of the experiment. Experiment 1 Reproductives produced ----------------------------------- Each reproductive individual identified (and removed). Colony = ID code of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. Date = date of inspection when individual was identified. Given in format ddmmyyyy. Sex = sex of individual, where G = gyne (new queen) and M = male. Experiment 1 Wax canopy coverage -------------------------------- Colony = ID code of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. WaxCover = Percentage cover over the nest comb of wax canopy. Rounded to nearest 5%. Assessed on day 27 of the experiment. Experiment 1 Wing fanning ------------------------- Colony = ID code of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. Date = date of the video recording. Given in format ddmmyyyy. Day = same as Date, but expressed in days since the beginning of the experiment. Time = length, in minutes, of video recording. BroodCover = percentage of focal area with nest comb. Rounded to nearest 10%. Incidences = number of fanning events (lasting over 10s) observed across all individuals throughout the recording. MeanBees = average number of workers throughout the duration of the video, estimated by counting the number of workers in the focal area at 10-minute intervals and then calculating the mean of these values. IncidRatePerBee = the number of incidences per minute per bee. Calculated as (Incidences/Time)/Meanbees. Fanning = binary condition of whether fanning or no fanning occurred during the recording. ++++++++++++++++++++++++++++++++++++++++++++++++++ Experiment 2: ~~~~~~~~~~~~ Experiment 2 Colony size ------------------------ Colony = ID code of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. colony size per date = the number of workers in the colony calculated by estimating the total number of males + workers and then subtracting the estimated number of males from the same week. Given to exact number where number of workers < 20. Otherwise, rounded to the nearest 5. NA values are given for three colonies which ran out of syrup and so had to be exlucded after this time. Experiment 2 Colony death ------------------------ Dates when colonies first declined to 0 workers, or when colonies were excluded from the experiment (see paper). These data were used for the survival analysis in the paper. Colony = ID number of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. Death = whether the colony 'died' (yes) or was excluded before death (no). Time = the time, expressed in the day of experiment, when the colony died or was exlcuded. Experiment 2 Queen death ------------------------ Dates when the colony queen was first observed dead, or when the colony was excluded from the experiment before this time (see paper), for each colony. Also includes data for the census at which the number of workers declined to 10 or below. These data were used for the survival analyses in the paper. Colony = ID number of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. Death = whether the queen died (yes) or the colony was excluded before queen death (no). Time = the time, expressed as the day of experiment, when the colony died or was exlcuded. Wkr10 = whether the colony declined to 10 workers (yes) or the colony was excluded before this time (no). Time = the first census, expressed as the day of experiment, when the number of workers in the colony declined to 10 or fewer workers. Experiment 2 Reproductives produced ----------------------------------- Number of reproductives produced per colony Colony = ID number of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. EGynesTot = total number of gynes (new queens) produced early in the experiment (before day 58, when 3 colonies ran out of syrup). EMalesMax = maximum number of males counted at any one census date early in the experiment (before day 58, when 3 colonies ran out of syrup). CGynesTot = total number of gynes (new queens) produced over the complete experiment. CMalesMax = maximum number of males counted at any one census date over the complete experiment. Experiment 2 Male count over time ------------------------ Colony = ID code of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. male count per date = the number of males estimated to be in the colony at a census date. NA values are given for three colonies which ran out of syrup and so had to be excluded after this time. Experiment 2 Worker longevity ----------------------------- The longevity of marked workers. Also included are data for a number of males which were marked mistakenly. Colony = ID code of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. ID = ID code of the marked adult MarkDate = date when the the adult was marked (<24h after eclosion). Cohort = which of two cohorts of eclosion date was the adult marked in. Where cohort 1 = eclosion on days 1-15, and cohort 2 = eclosion on days 20-29 of the experiment Sex = indicates whether male (data not analysed in the paper) or worker. DeathDate = date of adult death. Given in format ddmmyyyy. LastSeenDate = date when adult was last observed alive. Given in format ddmmyyyy. EAssumption = longevity of the adult, given earliest assumption of adult death (i.e. the date the adult was last seen alive). In days after eclosion. LAssumption = longevity of the adult, given latest assumption of adult death (i.e. the date the adult was first seen dead). In days after eclosion. MAssumption = longevity of the adult, given an adult death date as the mean of Eassumption and Lassumption. This was the metric analysed in the paper. In days after eclosion. Interval = time in days between DeathDate and LastSeenDate. Experiment 2 Worker production ------------------------------ Colony = ID number of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. Callows = number of callow workers observed from days 1-49 of the experiment. Experiment 2 Nest temperatures ------------------------------ Colony = ID number of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. Logger = identity of datalogger (B1 or B2) placed in the nest box, contacting brood, used to measure temperature during the measurement session. StartDate = date at the beginning of the measurement session. Given in format ddmmyyyy. StartDay = same as StartDate, but expressed in days since the beginning of the experiment. Tmin = minimum temperature (degrees Celcius) recorded during the session (approx 45h). Tmax = maximum temperature (degrees Celcius) recorded during the session (approx 45h). Tmean = mean temperature (degrees Celcius) recorded during the session (approx 45h). Measurement = the replicate ID of the session for the colony (i.e. whether the 1st, 2nd or 3rd session). Experiment 2 Wax canopy coverage -------------------------------- Colony = ID number of colony. Treatment = whether the colony was exposed to 20 or 25 degrees Celcius during the experiment. WC1 = Percentage cover over the nest comb of wax canopy. Rounded to nearest 5%. Assessed on day 15 of the experiment. WC2 = Percentage cover over the nest comb of wax canopy. Rounded to nearest 5%. Assessed on day 27 of the experiment.