The following help file is in regards to the manuscript: “The importance of phytoplankton biomolecule availability 6 for secondary production” Elina Peltomaa1,2*, Sanni L. Aalto3, Kristiina Vuorio4, Sami J. Taipale3 1Department of Environmental Sciences, University of Helsinki, P.O. Box 65, 00014 University of Helsinki, Finland 2Lammi Biological Station, University of Helsinki, Pääjärventie 320, 16900 Lammi, Finland 3Department of Biological and Environmental Science, University of Jyväskylä, P.O.Box 35 (YA), 40014 Jyväskylä, Finland 4Freshwater Centre, Finnish Environment Institute (Syke), Mechelininkatu 34a, P.O.18 Box 134, FI-00251 Helsinki, Finland Corresponding author: Elina Peltomaa, Lammi Biological Station, University of Helsinki, Pääjärventie 320, 16900 Lammi, Finland; e-mail: elina.peltomaa@helsinki.fi; phone: + 358 50 448 6649 The xlsx-file “phytoplankton_data” contains all results of elemental and biomolecule analysis of the studied algae and cyanobacteria. These results were used for Table 1 and Figures 1A-F. The xlsx-file “daphnia_data” contains the results (survival, neonata/egg production, survival rate) of feeding experiments with different algae and cyanobacteria. These results were used for Figures 2A and B.