Pos = Position of RNA sample in the 386 well plate Name = indicates the treatment Cp = Crossing point. Equivlent to threshold cycle. Gene = either the gene of interest or the endogenous control gene. ttbp is Tata binding protein, and the endogenous control. Social Environment = social environment treatment Sex = Sex of beetle Technical Rep = technical replicate number Biological Rep = biological replicate number Mean Cp tech reps = the mean of the technical reps for a given sample DCT = delta Ct which is based on calculations from Livat and Schmittgen 2001 (reference 71 in the paper). Gene of interest - endogenous control for a given sample. Mean of calibrator = mean of the delta Ct values. DDCT is delta delta Ct = this is calculated as delta Ct - mean of comparison control. That makes virgin have a mean delta delta Ct of zero. So looking at expression relative to average virgins. Relative expression is just log(2) raised to the -delta delta Ct. delta delta Ct = log transformed relative expression.