Target.Name Factor with two levels of either parasite-gene fragment (TB; Tetracapsuloides bryosalmonae) or host-gene fragment (ST; Salmo trutta) Plate Factor with 83 levels of 83 different 96-well qPCR plates Well Factor with 96 levels for the individual wells on each qPCR plate Sample.Name Factor with 696 levels for either one of four dilutions of a reference sample (0.1, 1, 10, 100) or one of 692 trout individuals Cq.Miner Continuous variable for the quantification cycle as inferred by the online tool 'Real-time PCR Miner' http://miner.ewindup.info/ Task Factor with two levels of either a trout individual ("UNKNOWN") or a reference dilution ("STANDARD") Plate.Sample.Name Factor with 1218 levels for the interaction of Plate and Sample.Name column Factor / continuous variable for the integer indicating the qPCR plate column from 1 to 12 row Factor / continuous variable for the integer indicating the qPCR plate row from 1 to 8 row.letter Factor for the letter indicating the qPCR plate row from A to H Plate.Well Factor with 7219 levels indicationg each measurement datum by technical Plate by Well interaction slope Continuous variable for linear-mixed model-predicted slope of Cq vs. log10 dilution for the reference dilutions, for each Gene by Plate combination SlopeEfficiency Continuous variable that is obtained as 10^(-1/slope) Ln2Cq.Miner.slope Continuous variable that is obtained as log2(SlopeEfficiency^Cq.Miner), i.e., the log2-standardized, efficiency-corrected quantification cycle