ReadMe


The file Boessenkool_Blocker.txt contains the final dataset from the experiment published in the associated article. Sequences and their inferred taxonomic identity are presented in tabulated format. For explanations of the sample names and the analyses applied to infer the taxonomic identity, we refer to the Materila and Methods in the associated article. 
The file contains the following columns:
-	id = a unique sequence identifier
-	sample:DY1_blk = number of reads of the sequence in sample DY1 amplified with blocking primer
-	sample:DY1_blk = number of reads of the sequence in sample DY1 amplified without blocking primer
-	sample:MR1_bl = number of reads of the sequence in sample MR1 amplified with blocking primer
-	sample:MR1_blk = number of reads of the sequence in sample MR1 amplified without blocking primer
-	sample:MR2_blk = number of reads of the sequence in sample MR2 amplified with blocking primer
-	sample:MR2blk = number of reads of the sequence in sample MR2 amplified without blocking primer
-	sample:MR3_blk = number of reads of the sequence in sample MR3 amplified with blocking primer
-	sample:MR3_blk = number of reads of the sequence in sample MR3 amplified without blocking primer
-	order_taxid = taxonomic identity of the identified order
-	order_name = name of the identified order
-	family_taxid = taxonomic identity of the identified family
-	family_name = name of the identified family
-	genus_taxid = taxonomic identity of the identified genus
-	genus_name = name of the identified genus
-	species_taxid = taxonomic identity of the identified species
-	species_name = name of the identified species
-	taxid = taxonomic identity of the scientific name
-	rank = level of identification (order, family, etc.)
-	scientific_name = final identification based on GenBank (can be an order, family, tribe, genus, species, etc.)
-	best_identity = best match with the closest sequence in the reference database (GenBank)
-	best_match = GenBank accession number of the closest sequence in the reference database
-	count = number of reads of the sequence in all the samples combined
-	sequence = DNA sequence


All data was collected by Sanne Boessenkool (sanneboessenkool@gmail.com) and Laura Epp (laura.epp@nhm.uio.no).