This archive contains 646 double channel TIFF images of wild-type Drosophila embryos that have undergone fluorescent in situ hybridisation for 32 pairwise combinations of pair-rule gene and segment-polarity gene transcripts. Most embryos are between mid-stage 5 and early stage 7.
Images are 1024x1024 pixels, and were collected on a Leica SP5 confocal microscope. The unpacked archive is 5.15 GB.
The gene combinations are:
- All pairwise combinations of the 7 canonical pair-rule genes (eve, ftz, hairy, odd, prd, run, slp)
- Two combinations with the non-canonical pair-rule gene odd-paired: opa/ftz; opa/odd
- Selected combinations including the segment-polarity genes engrailed, gooseberry and wingless: en/eve; en/gsb; en/odd; en/prd; en/slp; gsb/prd; wg/prd; wg/slp
Images for each gene combination are in a seperate folder. Each image shows an individual embryo, anterior left. Most images show a lateral view (dorsal top, ventral bottom). Occasional images showing a dorsal or ventral view are explicitly labelled as such. Images are roughly ordered by developmental age of the embryo.
File names are of the structure:
<green channel gene>_488_<red_channel_gene>_fr_<image number>.tif
(Alexa Fluor 488 was used to visualise the first gene, a Fast Red reaction was used to visualise the second gene. In general, the green channel gives a more sensitive readout of the expression pattern, but is also more prone to background.)
Questions about this dataset can be sent to Erik Clark: ec491@cam.ac.uk