Kristine Dunker Adam Sepulveda Robert Massengill Jeffrey Olsen Ora Russ John Wenburg Anton Antonovich Quantitative PCR results of Northern pike presence in water samples Kristine J. Dunker, Adam J. Sepulveda, Robert L. Massengill, Jeffrey B. Olsen, Ora L. Russ, John K. Wenburg, Anton Antonovich 2016 PLOSone PLOS We used field observations and experiments to test the sensitivity of our Northern pike environmental DNA (eDNA) assay and to evaluate the persistence of detectable DNA emitted from Northern pike carcasses. We then used eDNA sampling and traditional sampling (i.e., gillnets) to test for presence of Northern pike in four lakes subjected to a piscicide-treatment designed to eradicate this species. The dataset displays raw quantitative PCR results for Northern pike DNA for each of these experiments. Data were obtained to to test the sensitivity of our Northern pike environmental DNA (eDNA) assay, to evaluate the persistence of detectable DNA emitted from Northern pike carcasses, and to test for presence of Northern pike in four lakes subjected to a piscicide-treatm 20130603 20150528 ground condition Complete Not planned Data were collected in Alexander Lake of the Susitina River drainage and in Denise, Gensle, Little Bear, Tiny, Derks, East Mackey, Union, and West Mackey Lakes of the Soldotna Creek drainage of southcentral Alaska -153.67675780636 -148.05175780659 61.980267263552 59.175928247761 Invasive species Alaska, USA; carcass; eDNA; fish; genetics; invasive species; lake; Kenai Peninsula; rotenone GNIS Kenai Peninsula USGS Biocomplexity Thesaurus Fishes Adam Sepulveda U.S. Geological Survey mailing and physical

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Bozeman MT 59715 USA 406 994 7975 asepulveda@usgs.gov genetic analysis All species were completely and unambiguously identified. Northern pike none Adam Sepulveda U.S. Geological Survey mailing and physical

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Bozeman MT 59715 USA 406 994 7975 asepulveda@usgs.gov Internal positive controls were run for all reactions to identify negative results due to PCR inhibition. However, standard curves were not run for our analyses because our original objective was to only score samples as positive or negative. No formal logical accuracy tests were conducted Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. No formal positional accuracy tests were conducted No formal positional accuracy tests were conducted both We used a step-wise field approach to test the efficacy of eDNA as a detection tool for pike presence after eradication efforts. First, we used field observations and experiments to test if our eDNA assay could detect a large free-roaming pike population and low-density caged pike populations. Second, we evaluated how sampling distance from caged pike affected eDNA detection in order to inform field sampling strategies. Third, we assessed the persistence of pike DNA in lakes following pike carcass stocking. Olsen JB, Lewis CJ, Massengill RL, Dunker KJ, Wenburg JK 2015 Publication http://download.springer.com/static/pdf/650/art%253A10.1007%252Fs12686-015-0459-x.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs12686-015-0459-x&token2=exp=1472071693~acl=%2Fstatic%2Fpdf%2F650%2Fart%25253A10.1007%25252Fs12686-015-0459-x.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Farticle%252F10.1007%252Fs12686-015-0459-x*~hmac=1dbc39c3c02c394f130e39ba808c2d542aa132112644cd1ffa273f7faf3b97bc None 20160301 Raw_data Quantification cycle values for samples ran in replicate Ora Russ with U.S. Fish & Wildlife Service ID Number associated with each sample Producer defined 193 1414 Location Lake name, quadrant, and replicate number of water sample Produced defined Lake name, quadrant (N,S, E or W), and replicate number Call Samples that were scored positive (+) or negative (-) for pike DNA Produced defined Positive (+) and negative (-) RX.Y X indicates the quantitative PCR run, where samples were run 1 - 3 times. Y indicates the replicate number for each run. For example, R2.1 indicates the first replicate of a sample analyzed in run 2. Produced defined X indicates run number (1, 2 or 3). Y indicates replicated number within a run (1 - 12). RX_Call Samples that were scored positive (+), negative (-), or inconclusive (rerun) for pike DNA after a run. Where X indicates the run number. Producer defined X indicates the run number (1, 2 or 3). RX_IPC IPC = Internal Positive Control. IPC's were analyzed for each run (X) and scored as detect (*) or no-detect ( ). Producer defined PC's were analyzed for each run and scored as detect (*) or no-detect ( ). Adam Sepulveda U.S. Geological Survey mailing and physical

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Bozeman MT 59715 USA 406 994 7975 asepulveda@usgs.gov NA 20160824 Adam Sepulveda U.S. Geological Survey mailing and physical

2327 University Way Suite 2

Bozeman MT 59715 USA 406 994 7975 asepulveda@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999