Dryad
Published December 15, 2017 | Version v1
Dataset Open

Data from: Epithelial magnesium transport by TRPM6 is essential for prenatal development and adult survival

Creators

  • 1. Ludwig Maximilian University of Munich
  • 2. German Center for Diabetes Research
  • 3. German Center for Lung Research
  • 4. Princeton Medical Institute
  • 5. University of Würzburg
  • 6. University of Queensland

Description

Mg2+ regulates many physiological processes and signalling pathways. However, little is known about the mechanisms underlying the organismal balance of Mg2+. Capitalizing on a set of newly generated mouse models, we provide an integrated mechanistic model of the regulation of organismal Mg2+ balance during prenatal development and in adult mice by the ion channel TRPM6. We show that TRPM6 activity in the placenta and yolk sac is essential for embryonic development. In adult mice, TRPM6 is required in the intestine to maintain organismal Mg2+ balance, but is dispensable in the kidney. Trpm6 inactivation in adult mice leads to shortened lifespan, growth deficit and metabolic alterations indicative of impaired energy balance. Dietary Mg2+ supplementation not only rescues all phenotypes displayed by Trpm6-deficient adult mice, but also may extend the lifespan of wildtype mice. Hence, maintenance of organismal Mg2+ balance by TRPM6 is crucial for prenatal development and survival to adulthood.

Notes

Whole genome profiling of hepatic transcripts altered in Trpm6-deficient mice
Excel file contains two worksheets: (1) Genome-wide analysis of hepatic transcriptome in control vs Trpm6-deficient mice. (2) Up- and down-regulated transcripts in the liver of Trpm6-deficient mice with the false discovery rate (FDR) P≤0.1.
Table S2.xls
Ingenuity Pathway Analysis (IPA) analysis of hepatic transcripts altered in Trpm6-deficient mice
Excel file contains two worksheets: (1) IPA Canonical Pathways representing differentially expressed genes in the liver of Trpm6-deficient mice. (2) IPA Causal Networks for differentially expressed genes in the liver of Trpm6-deficient mice.
Table S3.xls
Metabolic profiling of the serum, liver and gastrocnemius muscle of Trpm6-deficient mice
Excel file contains seven worksheets: (1) Statistical analysis of metabolite measurements in serum samples. (2) Statistical analysis of metabolite measurements in gastrocnemius muscle samples. (3) Statistical analysis of metabolite measurements in liver samples. (4) Metabolites significantly changed in serum samples of Trpm6-deficient mice (FDR P≤0.05). (5) Metabolites significantly changed in gastrocnemius muscle samples of Trpm6-deficient mice (FDR P≤0.05). (6) Metabolites significantly changed in liver samples of Trpm6-deficient mice (FDR P≤0.05). (7) Abbreviations of metabolites.
Table S4.xlsx
MATLAB code
MATLAB code used for statistical analysis of survival distributions of control and dietary treated mice
MATLAB Code.docx

Files

Files (4.6 MB)

Name Size Download all
md5:e7c07e15a92ff3909234918901bcb218
14.4 kB Download
md5:ab0649b25fec77a688a7131842c85155
4.4 MB Download
md5:d1e819bb8f0da19cf6be8911d600e79e
100.4 kB Download
md5:b04809c48ee9e9a72ba2ee89a19d8a69
79.9 kB Download

Additional details

Related works

Is cited by
10.7554/elife.20914 (DOI)