README This is a raw hologram video sequence recorded on the instrument described in the referencing paper. It is an off-axis system, where the holograms may be reconstructed into intensity and phase images. Several software packages exist for hologram reconstruction. See the following to get started: http://onlinelibrary.wiley.com/doi/10.1002/9781118562567.app1/pdf For MATLAB: HoloRec3D (free package) http://sourceforge.net/directory/os:mac/?q=digital%20hologram%20reconstruction All of the holograms associated with this publication use the same Fourier mask, which is provided as a tiff file. For this specific file, the timestamps for the video are given on the following page. The z-planes used in the still images in the paper are the following: z= ?10 to 14. Reconstructions and more information may be found on our public database, motility.caltech.edu. We encourage you to contact us with any questions you may have about the data, the instrument, or the reconstructions. 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