"Treatment" Sulphide treatment of either no sulphide (Control) or 15 uM sulphide (Sulfide) "Family" Identifier for one of four Ciona intestinalis families (a, b, c, d) "Stage" Identifier for one of four developmental stages of Ciona intestinalis (Egg, 32.cell, Gastrula, Neurula, Tailbud) "Ln2Cq.HKGadj" Threshold qPCR detection value (Cq) for AOX mRNA, standardised for the geometric mean of two housekeeping genes