Description

Apply histogram matching bleaching correction to a specified image. This adjusts intensities in all frames (after the first) to match the histogram distribution of the first frame. It is intended to account for any fluorophore bleaching that occurs during acquisition of a timecourse.

This macro runs the Fiji bleaching correction plugin, "Bleach Correction", in histogram matching mode.

Parameters

Input image (default = "") Image from workspace to apply bleaching correction process to.

Apply to input image (default = "true") When selected, the post-operation image will overwrite the input image in the workspace. Otherwise, the image will be saved to the workspace with the name specified by the "Output image" parameter.

Output image (default = "") If "Apply to input image" is not selected, the post-operation image will be saved to the workspace with this name.