myc-tagged Fhl1 with deletion of forkhead domain (Fhl1-ΔFH-Myc)
W303 (Z1256); GCN4-myc; RTG3-knockout (co-factor)
strain: BY4741 MATa his3{delta}1 leu2{delta}0 met15{delta}0 ura3{delta}0 pEGH 2{mu} URA3 promGAL1-10-GST-GCN4
Treatment: sulfometuron added; Growth: for mid - long fase(OD600 about 0.6 to 0.8); Genotype: MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0; Strain: BY4741
Treatment: without adding sulfometuron; Growth: for mid - long fase(OD600 about 0.6 -0.8); Genotype: MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0; Strain: BY4741
HSF1 TAP-tagged yeast cells; none; temperature: 39 C
Treatment: 5 min heat shock, 39 degree; Genotype: Wildtype;
Treatment: 120 min heat shock, 39 degree; Genotype: Wildtype;
wild type (mock-treated by addition of YEPD)
0.5 hrs after treatment with H2O2; Snt2-13Myc strain;
W303 (Z1256); SWI4-myc; SWI6-knockout (co-factor)