Explanation of files for Calhoun et al. "Testing the multiple stressor hypothesis: Chlorothalonil exposure alters transmission potential of a bumblebee pathogen but not individual host health"

File: Chlorothalonil_no_choice_reference_experiment_data.csv

Data from experiment testing preference of bees between control and chlorothalonil spiked sugar water over time when given no choice. Analysis script: Script_Clorothalonil_Preference_Combined.R

ID: Sample ID
SourceColony: Unique lab ID for colony of origin of each worker bee
Treatment: Control or Chlorothalonil-spiked sugar water
Day_2_CHL_ms1: Weight (g) at end of time period 1 (0-2 days) - technical replicate 1
Day_2_CHL_ms2: Weight (g) at end of time period 1 (0-2 days) - technical replicate 2
mean_diff_day_2: Weight decrease (consumption) relative to non-consumed standards for time period 1 (0-2 days)
Day_6_CHL_ms1: Weight (g) at end of time period 2 (3-6 days) - technical replicate 1
Day_6_CHL_ms2: Weight (g) at end of time period 2 (3-6 days) - technical replicate 2
mean_diff_day_6: Weight decrease (consumption) relative to non-consumed standards for time period 1 (3-6 days)
Survived: If bees survived (1) or not (0) both time periods
Missing values are denoted by NA

File: Chlorothalonil_choice_reference_experiment_data.csv

Data from experiment testing preference of bees between control and chlorothalonil spiked sugar water over time when given a choice. Analysis script: Script_Clorothalonil_Preference_Combined.R

ID: Sample ID
colony: Unique lab ID for colony of origin of each worker bee
Treatment: Control or Chlorothalonil-spiked sugar water
Time_period: Time period 1 (0-2 days, Day 2) or Time period 2 (3-6 days, Day 6)
mean_diff: Weight decrease (consumption) relative to non-consumed standards

File: Microcolony_level_data.csv

Microcolony level data from experiment investigating individual and combined effects of pathogen and chlorothalonil exposure. Analysis script: Script_microcolony_data.R

source: Unique lab ID for colony of origin of each microcolony
ucolony: Unique microcolony ID
nosema: Treatment of Nosema, exposed (1) or not (0)
chlorothalonil: Treatment of chlorothalonil, exposed (1) or not (0) 
pp_d1_grams: Consumed pollen pellet (g) by microcolony at time period 6-8days post-start 
pp_d2_grams: Consumed pollen pellet (g) by microcolony at time period 8-10days post-start 
pp_d3_grams: Consumed pollen pellet (g) by microcolony at time period 10-12days post-start 
SW_d1_mL: Consumed sugar water (ml) by microcolony at time period 6-8days post-start 
SW_d2_mL: Consumed sugar water (ml) by microcolony at time period 8-10days post-start
SW_d3_mL: Consumed sugar water (ml) by microcolony at time period 10-12days post-start
larvae_start: Larvae present at inoculation per microcolony
larvae_emerged: Adults emerging by 37days post microcolony start
Missing values are denoted by NA

File: Chlorothalonil_Nosema_individual_data.csv
Data from adults emerging from worker microcolonies with exposure to Nosema bombi or Chlorothalonil. Measures include individual development time, size, protein amounts, infection and spore loads of Nosema, and survival. Analysis script: Script_Clorothalonil_Nosema_individual.R

source_colony: Unique lab ID for colony of origin of each microcolony
microcolony: Unique microcolony ID
ID: Sample ID
nosema: Treatment of Nosema, exposed (1) or not (0)
chlorothalonil: Treatment of chlorothalonil, exposed (1) or not (0) 
purpose: used for survival, or sampled before for qPCR or protein assay (biochem). Biochem bees also check for infection and added to qPCR set.
initiation: date of microcolony oviposition
emergence: individual adult emergence date
death: individual death date
development: days from oviposition to adult emergence
survival: days of adult survival
death_y_n: if individual was censored (0) or died (1)
body_size: body size based on surrogate of forewing radial cell length (mm)
protein_ug_ml: protein amount per sample
total_inf: total infection per bee based on qPCR
extra_spores: total extracellular spores per bee
prop_spores: proportion spores in total infection
total_bi: Infected (1) or not (0)
extra_bi: Spores (1) or not (0)
infection: evidence of infection (1) or not (0)
Missing values are denoted by NA