Published March 19, 2021
| Version v1
Dataset
Open
Validating eDNA Measurements of the Richness and Abundance of Anurans at a Large Scale
Creators
- 1. Chinese Academy of Sciences
- 2. Anhui Normal University
- 3. Lishui University
- 4. Nanjing Normal University
Description
- In some situations, environmental DNA (eDNA) metabarcoding is a more accurate tool for measuring species richness of a taxon than conventional methods. Whether this tool can reliably estimate the abundance of a taxon remains unresolved.
- We examined the reliability of metabarcoding for measuring anuran diversity compared to a commonly used traditional line transect method (TLTM) through the replicate sampling of three visits across 71 waterbodies (ponds or reservoirs) in Liuheng, China. We also investigated the relative contributions of species-specific characteristics and the physiochemical properties of a waterbody on the relative read count across species and waterbodies.
- We found that eDNA metabarcoding had a higher detection probability for each of seven anuran species found in the sampling region than TLTM. Furthermore, the relative read count estimated by metabarcoding was positively correlated with the density or relative density of individuals identified with the TLTM across waterbodies for every species. Species-specific characteristics of anurans, such as density, relative density, body mass, biomass and relative biomass, accounted for substantial variations in the read count across species and waterbodies, while physiochemical factors, including pH, temperature, water volume, vegetation and elevation, had little effect on the read count.
- Our results based on robust sampling suggest that metabarcoding enables more reliable and efficient measurements of anuran occurrence at a large scale during a short-term survey (within 15 days) than that obtained by the TLTM, and offers an alternative tool for quantifications of anuran abundance. Density or biomass is better and more reliable indicator of anuran abundance associated with read count than relative density or relative biomass.
Notes
Funding provided by: National Natural Science Foundation of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809
Award Number: 3,203,007,031,872,240
Files
0_1.zip
Files
(8.7 GB)
| Name | Size | Download all |
|---|---|---|
|
md5:3d26486e4e485c7e166d82acd7c35b45
|
5.7 MB | Download |
|
md5:123cabbcbcb7b5d3a183d37109da0baa
|
494.1 MB | Preview Download |
|
md5:c69cdfd39e1fea8f9f87e225457dfdaf
|
576.8 MB | Preview Download |
|
md5:41dd699fa5affabfdb1127d17a018960
|
5.4 MB | Download |
|
md5:12e141d51f1ab9b703e634d8a3b24379
|
502.4 MB | Preview Download |
|
md5:93225f0e0af184f4d1affdb887f63880
|
577.0 MB | Preview Download |
|
md5:84bf8b82951947dbf6002c06462f4a8a
|
4.5 MB | Download |
|
md5:9e49dbebc6faeed27ef2f0f865228873
|
416.9 MB | Preview Download |
|
md5:cc7db3cbe2a9e30d3299561a94e7d827
|
474.6 MB | Preview Download |
|
md5:b5ee295c36ec74199910aeeed0513c5e
|
6.0 MB | Download |
|
md5:9d27b0d4278af2d73304810daf635b88
|
382.1 MB | Preview Download |
|
md5:5b4747e07a80786b3c1b3e3e13bdca7a
|
423.7 MB | Preview Download |
|
md5:7030ab855b425a24630e0f56f15003c5
|
5.7 MB | Download |
|
md5:7088f2abe32cff789cd0ab770721ce23
|
496.6 MB | Preview Download |
|
md5:6ac830382b02191bca1723c239bcb260
|
558.3 MB | Preview Download |
|
md5:ac970790aa3a62c2763133cac3cf24b0
|
5.0 MB | Download |
|
md5:b83f8e0e12f0f77c6f1e41d92c3474a1
|
357.6 MB | Preview Download |
|
md5:a57422ca4330ee4320be51e2978af78d
|
408.8 MB | Preview Download |
|
md5:bbf92ae5df30ac03650114e2f2029f70
|
6.3 MB | Download |
|
md5:364f6c390e5d11e1a302e63508d6f90a
|
396.9 MB | Preview Download |
|
md5:67d79b0a0423792ef605c6ac6e7605ea
|
441.7 MB | Preview Download |
|
md5:7090cbc25f6e1234163d914add803bd8
|
5.6 MB | Download |
|
md5:e5f116f97891f316708f702af470cb12
|
328.3 MB | Preview Download |
|
md5:add258975d5fef3a33fa67126a775723
|
369.4 MB | Preview Download |
|
md5:ad8b634ca5497104e53ee8c863c84ef8
|
4.0 MB | Download |
|
md5:28acd0577b8e7306166a6f0482239e1c
|
313.8 MB | Preview Download |
|
md5:c5be29a20672b8e74fd916bd51f39372
|
367.6 MB | Preview Download |
|
md5:0d4e213f588ffc65fdd930dd1d7c3f88
|
3.8 MB | Download |
|
md5:d3a481938acbb0af9247a3676f1c6a26
|
347.7 MB | Preview Download |
|
md5:a1bea544be0227f9ad8334d75a6ad001
|
392.5 MB | Preview Download |
|
md5:fc4ee8ba9dcb6a39802e25ec5c9f1a22
|
1.3 kB | Preview Download |
|
md5:4ee44ef0cffd82744f2a4147d4386622
|
194 Bytes | Preview Download |
|
md5:0937e08529a3cc6bea0583516ae525bc
|
1.0 kB | Preview Download |
|
md5:3e3bdb1fd27f0a59f70f15de29b6595d
|
1.6 kB | Preview Download |
|
md5:b2695ea2e945199e28c6103f6669acf7
|
1.3 kB | Preview Download |
|
md5:cad2c3d7064725e963b5cd4348ff7bf3
|
1.2 kB | Preview Download |
|
md5:c7e541e84a000ed3e056266a66153aef
|
345 Bytes | Preview Download |
|
md5:984f7b130ad6ea9da5588bdea66ca7e4
|
1.0 kB | Preview Download |
|
md5:14049098795520f3ea381e0547cd2812
|
2.5 kB | Preview Download |
|
md5:803e9791721ff2bee8a267da79aee925
|
28 Bytes | Preview Download |
|
md5:313cebbc8fd64323af4e27dde69f4be4
|
1.0 kB | Preview Download |
|
md5:e125ea9a24e35c5afcf57a56f67431a1
|
91 Bytes | Preview Download |
|
md5:0403b074d06f109f74872f2ca45bc35c
|
1.0 kB | Preview Download |
|
md5:828d81bbddddff19394926e9b6d437ae
|
9.1 kB | Download |
|
md5:6e9b24a25e8d333c72efaec39f7dce4f
|
243 Bytes | Preview Download |
|
md5:3f2191858314befd2c6838e80006ca1b
|
1.0 kB | Preview Download |
|
md5:cea43dd1ffea66e02c183a6d7767c6c2
|
1.9 kB | Preview Download |
|
md5:3fd52c08befd002d9ba6ad46e5b3024f
|
242 Bytes | Preview Download |
|
md5:148f4c2ef47e5cd461611b7e5d4b0df3
|
383 Bytes | Preview Download |
|
md5:95ba6e45ce0ff870e9e529ae56eeede4
|
1.0 kB | Preview Download |
|
md5:6f15ab1f08d02355a74e578c702779f8
|
732 Bytes | Preview Download |
|
md5:e4c74612d9bfce998ebba45bc76d8fd9
|
298 Bytes | Preview Download |
|
md5:dd6a3b788be18d24dc72bb50bf5226fd
|
1.0 kB | Preview Download |
|
md5:58cf31994fd4d796af8487842e65b537
|
14.9 kB | Download |
|
md5:248b853591c2fa022d6b6593c7098ee5
|
508 Bytes | Preview Download |
|
md5:d565afd9505f99a32ef2de746e70122b
|
1.9 kB | Preview Download |
|
md5:aee3e2451056ae061adca8e153cecf0f
|
453 Bytes | Preview Download |
|
md5:282be608bcfebf08604a4a71b2029e60
|
713 Bytes | Preview Download |
|
md5:a65ac2324cef2ac5ec2d4bc17adb41a7
|
711 Bytes | Preview Download |
|
md5:a40a2492091d51e40c52695e845c17ae
|
715 Bytes | Preview Download |
|
md5:35940c6529272ecf1874ad6529c1a095
|
713 Bytes | Preview Download |
|
md5:0ef07b06cddcb4fde427b2476159f32c
|
709 Bytes | Preview Download |
|
md5:5d6ceeeaa20e644c4e9cd195e142c1e5
|
714 Bytes | Preview Download |
|
md5:ff64e54ab3c73d7ec787c2760e9f07dd
|
715 Bytes | Preview Download |
|
md5:9000d340c0803f8d1ed2bde3bc085d42
|
711 Bytes | Preview Download |
|
md5:20fecd29feda403cf20f86e96e2a3b48
|
709 Bytes | Preview Download |
|
md5:8bdf0029bd91ca45de06c0e05609e408
|
1.9 kB | Preview Download |
|
md5:94addc730f5a2050d9c33268f3cddd0c
|
1.3 kB | Preview Download |
|
md5:5809a12781114bf90b6c06334a45fb73
|
3.5 kB | Preview Download |
Additional details
Related works
- Is derived from
- 10.5281/zenodo.4575518 (DOI)