Published March 4, 2021 | Version v1
Dataset Open

Coral reef fish Amphiprion ocellaris responses to ocean warming: lipid contents, lipid peroxidation and fatty acid profiles in liver and muscle

  • 1. UCIBIO – Applied Molecular Biosciences Unit, NOVA School of Science and Technology, 2829-516 Caparica, Portugal
  • 2. CESAM – Centre for Environmental and Marine Studies, University of Aveiro, Edifício ECOMARE, Estrada do Porto de Pesca Costeira, 3830-565 Gafanha da Nazaré, Portugal
  • 3. Ecosystem Physiology, University of Freiburg, 53/54 Georges-Köhler Allee, 79119 Freiburg, Germany
  • 4. Centre for Ecology, Evolution and Biogeochemistry, Swiss Federal Institute of Aquatic Science and Technology (Eawag), Seestrasse 79, 6047 Kastanienbaum, Switzerland
  • 5. CCMAR - Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal

Description

In this study, we used clownfish Amphiprion ocellaris as a model organism to study reef fish mechanisms of thermal adaptation and determine how high temperature affects different lipid aspects in fish. We exposed juvenile fish to two different experimental conditions, implemented over 28 days: average tropical water temperatures (26 ˚C, control) or average warm pool temperatures (30 ˚C). We then performed several analyses on fish muscle and liver tissues: i) total lipid content (%), ii) lipid peroxides, iii) fatty acid profiles, iv) lipid metabolic pathways and v) body condition. Raw data from these analyses were compiled and included in three datasheets: one for the fatty acid masses/concentration datasets, the second for body condition (length and weight) dataset and the third for fatty acid enrichment dataset based on LMSD – Lipid Maps Structure Database; KEGG – Kyoto Encyclopedia of Genes and Genomes; and HMBD – Human Metabolome Database. Statistical analyses and conclusions from the study can be found in Madeira et al. 2021 entitled "Conserved fatty acid profiles and lipid metabolic pathways in a tropical reef fish exposed to ocean warming – an adaptation mechanism of tolerant species?", published in STOTEN, https://doi.org/10.1016/j.scitotenv.2021.146738

 

Notes

This work was funded by: i) the Portuguese Fundação para a Ciência e Tecnologia (FCT) through the projects PTDC/MAR-EST/2141/2012, UIDB/04292/2020 granted to MARE, UIDB/04326/2020 granted to CCMAR and UIDB/04378/2020 granted to UCIBIO; ii) CESAM (UIDP/50017/2020+UIDB/50017/2020) by FCT/MEC through national funds, and the co-funding by FEDER, within PT2020 Partnership Agreement and Compete 2020; iii) work in the organic chemistry laboratory at Eawag Kastanienbaum was funded by internal Eawag funds. CM was supported by a researcher grant CEECIND/01526/2018 provided by FCT. D.M. was supported by a researcher grant CEECIND/01250/2018, by FCT. M.L. was supported by the Integrated Programme of SR&TD "SmartBioR - Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate" (Centro-01-0145-FEDER-000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund.

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Is derived from
Journal article: 10.1016/j.scitotenv.2021.146738 (DOI)

Funding

Fundação para a Ciência e Tecnologia
PTDC/MAR-EST/2141/2012 – WarmingWebs - Role of biodiversity, species thermal tolerance and food web structure in the response to climate change: Temperate versus tropical ecosystems. 127742