Data collected by Amanda R. Meier and Mark D. Hunter 
Contact at armeier@umich.edu 

Files and descriptions 
AMFFieldExpt_Dryad.xlsx


Worksheet1: ProbabilityExtinctionWeeks1-6
Probability that aphid colonies would persist through the weeks of reduced predator pressure (Weeks 1-6). 

Block: spatial block of the experiment and temporal block in which the plant was harvested at the end of the experiment (1-4).

AMF: Arbuscular mycorrhizal fungi (AMF) treatment (i.e. the amount of AMF the plant was initially inoculated with - either Zero, Medium, or High.)

Species: Plant species Ð either Cur = A. curassavica or Inc = A. incarnata. 

PlantID: unique identifying code for each plant. 

ColonyExtinct: whether the aphid colony was driven extinct within the first 6 weeks of reduced predator pressure. 0 indicates that the colony persisted through the 6 weeks. 1 indicates that the colony went extinct within the first 6 weeks. 

Worksheet 2: Weeks1-6 
Survey of the abundance of aphids (Aphis nerii), aphid midge flies (Aphidoletes aphidimyza), spiders, coccinellids, lacewings, and syrphids during each week of reduced predator pressure (Weeks 1-6). Only plants on which aphid colonies persisted through the six weeks are included.

Block: spatial block of the experiment and temporal block in which the plant was harvested at the end of the experiment (1-4).

AMF: AMF treatment (the amount of AMF the plant was initially inoculated with - either Zero, Medium, or High.) 

Species: Plant species Ð either Cur = A. curassavica or Inc = A. incarnata. 

PlantID: unique identifying code for each plant. 

Height: plant height (cm)

Anerii: number of Aphis nerii aphids on the plant 

Midge: number of aphid midge fly (Aphidoletes aphidimyza) eggs and larvae on the plant. 

Spider: number of spiders on the plant. 

Coccinelid: number of coccinelid eggs, larvae and adults on the plant. 

Lacewing: number of lacewing eggs and larvae on the plant. 

Syrphid: number of syrphid eggs and larvae on the plant. 

Predators: cumulative number of predators on the plant. 


Worksheet 3: CumulativePredatorsWeeks1-6
Cumulative abundances of aphids (Aphis nerii), spiders, lacewings, syrphids, aphid midges flies, and coccinellids over the weeks of reduced predator pressure (Weeks 1-6). 

Block: spatial block of the experiment temporal block in which the plant was harvested at the end of the experiment (1-4).

AMF: AMF treatment (the amount of AMF the plant was initially inoculated with - either Zero, Medium, or High.)

Species: Plant species Ð either Cur = A. curassavica or Inc = A. incarnata. 

PlantID: unique identifying code for each plant. 

Anerii: cumulative number of aphids present on the plant in Weeks 1-6. 

Spider: cumulative number of spiders present on the plant in Weeks 1-6.

Lacewing: cumulative number of lacewing eggs and larvae present on the plant in Weeks 1-6.

Syrphid: cumulative number of syrphid eggs and larvae present on the plant in Weeks 1-6. 

Midge: cumulative number of midge eggs and larvae present on the plant in Weeks 1-6. 

Coccinelid: cumulative number of coccinelid eggs, larvae, and adults present on the plant in Weeks 1-6. 

Worksheet 4: Weeks6-9
Survey of aphids (Aphis nerii) and their predators under full predator pressure. We stopped removing predators in Week 6 and ended the experiment after Week 9.  

Block: spatial block of the experiment and temporal block in which the plant was harvested at the end of the experiment (1-4).

AMF: AMF treatment (the amount of AMF the plant was initially inoculated with - either Zero, Medium, or High.) 

Species: Plant species Ð either Cur = A. curassavica or Inc = A. incarnata. 

PlantID: unique identifying code for each plant. 

ColonyExtinctbyWeek9: Aphis nerii colony was driven to extinction by Week 9. 0 Ð colony never driven extinct in experiment, 1 Ð colony extinct by Week 9. 

AphidsWeek7: Number of aphids (Aphis nerii) on the plant in Week 7. 

AphidsWeek6: Number of aphids (Aphis nerii) on the plant in Week 6. 

AphidsKilledbyMidgesWeek7: Number of aphids killed by midges (i.e. distinct aphid carcasses) present on the plant in Week 7. 

MidgesWeek6: Number of midge eggs and larvae on the plant in Week 6. 

SyrphidsWeek6: Number of syrphid eggs and larvae on the plant in Week 6. 

LacewingsWeek6: Number of lacewing eggs and larvae on the plant in Week 6. 

CoccinelidsWeek6: Number of coccinellid eggs, larvae, and adults on the plant in Week 6. 

SpidersWeek6: Number of spiders on the plant in Week 6. 


Worksheet 5: PrelimPlantTraits
Plant traits of Asclepias incarnata and A. curassavica at the beginning of the experiment (mid-July: 3-month old plants). 

Block: spatial block of the experiment and temporal block in which the plant was harvested at the end of the experiment (1-4).

AMF: AMF treatment (the amount of AMF the plant was initially inoculated with - either Zero, Medium, or High. 

Species: Plant species Ð either Cur = A. curassavica or Inc = A. incarnata. 

PlantID: unique identifying code for each plant. 

PropCol: Proportion of plant roots colonized by any AMF structures. 

PropArb: Proportion of plant roots colonized by AMF arbuscules. 

PlantHeight: height of the plant (cm). 

PlantLeaves: number of leaves on the plant. 

AbovegroundBiomass = the dry weight of all aboveground plant tissue (mg).

BelowgroundBiomass = the dry weight of all belowground plant tissue (mg).

TotalCardenolides: concentration of total foliar cardenolides (mg cardenolides/g dry foliar tissue).

CardenolideDiversity: diversity of cardenolides present in leaves.

CardenolidePolarity: polarity of cardenolides present in leaves.

PercentCarbon = concentration of carbon in foliar tissue (%). 

PercentNitrogen = concentration of nitrogen in foliar tissue (%). 

PercentPhosphorous = concentration of phosphorous in foliar tissue (%). 

CNratio = foliar carbon/nitrogen ratio, calculated as PercentC/PercentN.

PropMoisture: proportion water content in leaves. 

LeafToughness = specific leaf mass (mg/cm^2), a proxy for leaf toughness


Worksheet 6: FinalPlantTraits
Traits of Asclepias incarnata and A. curassavica plants at the end of the growing (October: 5-month old plants). 

Block: spatial block of the experiment and temporal block in which the plant was harvested
(1-4). 

AMF: AMF treatment (the amount of AMF the plant was initially inoculated with - either Zero, Medium, or High).

Species: Plant species Ð either Cur = A. curassavica or Inc = A. incarnata. 

PlantID: unique identifying code for each plant. 

PropCol: Proportion of plant roots colonized by any AMF structures. 

PropArb: Proportion of plant roots colonized by AMF arbuscules. 

AbovegroundBiomass = the dry weight of all aboveground plant tissue (mg).

BelowgroundBiomass = the dry weight of all belowground plant tissue (mg).

TotalCardenolides: concentration of total foliar cardenolides (mg cardenolides/g dry foliar tissue).

CardenolideDiversity: diversity of cardenolides present in leaves.

CardenolidePolarity: polarity of cardenolides present in leaves.