Published May 17, 2019 | Version v1
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High-Elevation Chromosomal Diversity of Black Flies (Diptera: Simuliidae) in Thailand

  • 1. Department of Plant and Environmental Sciences, Clemson University, 130 McGinty Court, E143 Poole Agricultural Center,Clemson, SC29634-0310,
  • 2. EntomologySection,Queen Sirikit Botanic Garden,P.O.Box 7,Maerim,Chiang Mai 50180,Thailand,
  • 3. Tropical Infectious Diseases Research and Education Centre, University of Malaya,50603 Kuala Lumpur, Malaysia,
  • 4. Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200,Thailand, and


Adler, Peter H., Srisuka, Wichai, Low, Van Lun, Takaoka, Hiroyuki, Saeung, Atiporn (2019): High-Elevation Chromosomal Diversity of Black Flies (Diptera: Simuliidae) in Thailand. Insect Systematics and Diversity 3 (2019), No. 1: 1-10, DOI: 10.1093/isd/ixz004, URL:



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  • Adler, P. H. 1988. Ecology of black fly sibling species, pp. 63-76. In K. C. Kim and R. W. Merritt (eds.), Black flies: ecology, population management and annotated world list. Pennsylvania State University Press, University Park, PA.
  • Adler, P. H., and E. A. Kachvorian. 2001. Cytogenetics of the Holarctic black fly Simulium noelleri (Diptera: Simuliidae). Can. J. Zool. 79: 1972-1979.
  • Adler, P. H., D. C. Currie, and D. M. Wood. 2004.The Black flies (Simuliidae) of North America. Cornell University Press, Ithaca, NY.
  • Adler, P. H., Y. T. Huang, W. K. Reeves, S. K. Kim, Y. Otsuka, and H. Takaoka. 2013.Macrogenomic evidence for the origin of the black fly Simulium suzukii (Diptera: Simuliidae) on Okinawa Island, Japan. PLoS One 8: e70765.
  • Adler, P. H., O. Yadamsuren, and W. S. Procunier. 2016a. Chromosomal translocations in black flies (Diptera: Simuliidae)-facilitators of adaptive radiation? PLoS One 11: e0158272.
  • Adler, P. H., T. Kudelova, M. Kudela, G. Seitz, and A. Ignjatovic- Cupina. 2016b. Cryptic biodiversity and the origins of pest status revealed in the macrogenome of Simulium colombaschense (Diptera: Simuliidae), history's most destructive black fly. PLoS One 11: e0147673.
  • Adler, P. H., H. Takaoka, M. Sofian-Azirun, V. L. Low, Z. Ya'cob, C. D. Chen, K. W. Lau, and X. D. Pham. 2016c. Vietnam, a hotspot for chromosomal diversity and cryptic species in black flies (Diptera: Simuliidae). PLoS One 11: e0163881.
  • Brunetti, E. 1911. New Oriental Nemocera. Records of the Indian Museum 4: 259-316.
  • Choochote, W., H. Takaoka, M. Fukuda,Y. Otsuka, C. Aoki, and N. Eshima. 2005. Seasonal abundance and daily flying activity of black flies (Diptera: Simuliidae) attracted to human baits in Doi Inthanon National Park, northern Thailand. Med. Entomol. Zool. 56: 335-348.
  • Colbo, M. H., and G. N. Porter. 1981. The interaction of rearing temperature and food supply on the life history of two species of Simuliidae (Diptera). Can. J. Zool. 59: 158-163.
  • Gill, B. A., B. C. Kondratieff, K. L. Casner, A. C. Encalada, A. S. Flecker, D. G. Gannon, C. K. Ghalambor, J. M. Guayasamin, N. L. Poff, M. P. Simmons, et al. 2016. Cryptic species diversity reveals biogeographic support for the 'mountain passes are higher in the tropics' hypothesis.Proc R Soc B 283: 20160553.
  • Ilmonen, J., P. H. Adler, B. Malmqvist, and A. Cywinska. 2009. The Simulium vernum group (Diptera: Simuliidae) in Europe: multiple character sets for assessing species status. Zool. J. Linn. Soc. 156: 847-863.
  • Kier, G., H. Kreft, T. M. Lee, W. Jetz, P. L. Ibisch, C. Nowicki, J. Mutke, and W. Barthlott. 2009. A global assessment of endemism and species richness across island and mainland regions. Proceedings of the National Academy of Sciences, USA 106: 9322-9327.
  • Kumar, S., G. Stecher, and K. Tamura. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33: 1870-1874.
  • Kuvangkadilok, C., S. Phayuhasena, and C. Boonkemtong. 1999. Larval polytene chromosomes of five species of blackflies (Diptera: Simuliidae) from Doi Inthanon National Park, northern Thailand. Cytologia 64: 197-207.
  • Kuvangkadilok, C., C. Boonkemtong, S. Phayuhasena, and V. Baimai. 2003. Larval polytene chromosomes of black flies (Diptera: Simuliidae) from Thailand. Genetica 118: 69-81.
  • Lewontin, R. C. 1974. The genetic basis of evolutionary change. Columbia University Press, New York, NY.
  • Low, V. L., H. Takaoka, P. H. Adler, Z. Ya'cob, Y. Norma-Rashid, C. D. Chen, and M. Sofian-Azirun. 2015. A multi-locus approach resolves the phylogenetic relationships of the Simulium asakoae and Simulium ceylonicum species groups (Diptera:Simuliidae) in Malaysia: evidence for distinct evolutionary lineages. Med. Vet. Entomol. 29: 330-337.
  • Low, V. L., H. Takaoka, P. H. Adler, T. K. Tan, F. C.-H. Weng, C.-Y. Chen, Y. A. L. Lim, Z. Ya'cob, C. D. Chen, M. Sofian-Azirun, and D. Wang. 2018. A novel molecular and chromosomal lineage of the anthropophilic Simulium (Simulium) rufibasis subgroup (Diptera: Simuliidae) in Taiwan. Parasitol. Res. 117: 3137-3143.
  • McCain, C. M., and J.-A. Grytnes. 2010. Elevational gradients in species richness. In Encyclopedia of life sciences (ELS). John Wiley & Sons, Ltd, Chichester, United Kingdom.
  • Merckx, V. S. F.T., K. P.Hendriks, K. K. Beentjes, C. B. Mennes, L. E. Becking, K. T. C. A. Peijnenburg, A. Afendy, N. Arumugam, H. de Boer, A. Biun, et al. 2015. Evolution of endemism on a young tropical mountain. Nature 524: 347-350.
  • Miraldo, A., S. Li, M. K. Borregaard, A. Florez-Rodriguez, S. Gopalakrishnan, M. Rizvanovic, Z. Wang, C. Rahbek, K. A. Marske, and D. Nogues-Bravo. 2016.An Anthropocene map of genetic diversity. Science 353: 1532-1535.
  • Pramual, P., and S. Pangjanda. 2015. Effects of habitat specialization on population genetic structure of black fly Simulium weji Takaoka (Diptera: Simuliidae). J. Asia Pacific Entomol. 18: 33-37.
  • Pramual, P., and K. Wongpakam. 2013. Population genetics of the high elevation black fly Simulium (Nevermannia) feuerborni Edwards in Thailand. Entomol. Sci. 16: 298-308.
  • Pramual, P., C. Kuvangkadilok, V. Baimai, and C. Walton. 2005. Phylogeography of the black fly Simulium tani (Diptera: Simuliidae) from Thailand as inferred from mtDNA sequences. Mol. Ecol. 14: 3989-4001.
  • Robin, V. V., A. Sinha, and U.Ramakrishnan.2010.Ancient geographical gaps and paleo-climate shape the phylogeography of an endemic bird in the sky islands of southern India. PLoS One 5: e13321.
  • Rothfels, K. 1989. Speciation in black flies. Genome 32: 500-509.
  • Rothfels, K., and R. Nambiar. 1981. A cytological study of natural hybrids between Prosimulium multidentatum and P. magnum with notes on sex determination in the Simuliidae (Diptera). Chromosoma 82: 673-691.
  • Rothfels, K., R. Feraday, and A. Kaneps. 1978. A cytological description of sibling species of Simulium venustum and S. verecundum with standard maps for the subgenus Simulium Davies [sic] (Diptera). Can. J. Zool. 56: 1110-1128.
  • Sheldon, K. S., R. B. Huey, M. Kaspari, and N. J. Sanders. 2018. Fifty years of mountain passes: a perspective on Dan Janzen's classic article. Am. Nat. 191: 553-565.
  • Srisuka, W., H. Takaoka, Y. Otsuka, M. Fukuda, S. Thongsahuan, K. Taai, and A. Saeung. 2017. Biodiversity, seasonal abundance, and distribution of blackflies (Diptera: Simuliidae) in six different regions of Thailand. Parasites and Vectors 10: 574.
  • Swofford, D. L. 2002. PAUP* phylogenetic analysis using parsimony (*and other methods). Sinauer Associates, Sunderland, MA.
  • Takaoka, H., and W. Choochote. 2004. Two new species of Simulium (Simulium) (Diptera: Simuliidae) from Thailand. Tropical Medicine and Health 32: 31-36.
  • Takaoka, H., and H. Suzuki. 1984. The blackflies (Diptera: Simuliidae) from Thailand. Jpn. J. Sanit. Zool. 35: 7-45.
  • Takaoka, H., W. Srisuka, and A. Saeung. 2017a. Two new species of the Simulium (Simulium) variegatum species-group of black flies (Diptera: Simuliidae) from Thailand. J. Med. Entomol. 54: 1213-1223.
  • Takaoka, H., M. Sofian-Azirun, Z. Ya'cob, C. D. Chen, K. W. Lau, V. L. Low, X. D. Pham, and P. H. Adler. 2017b. The black flies (Diptera: Simuliidae) of Vietnam. Zootaxa 4261: 1-165.
  • Takaoka, H., W. Srisuka, V. L. Low, and A. Saeung. 2018a. Five new species of the Simulium decuplum subgroup of the Simulium (Gomphostilbia) batoense species-group (Diptera: Simuliidae) from Thailand and their phylogenetic relationships. Acta Trop. 182: 271-284.
  • Takaoka, H., W. Srisuka, V. L. Low, and A. Saeung. 2018b. Morphological and molecular analyses of Simulium rufibasis (Diptera: Simuliidae) in Thailand. J. Med. Entomol. In press.
  • Takaoka, H., W. Srisuka,V. L. Low, and A. Saeung. 2019. A new species of the Simulium (Simulium) multistriatum species-group (Diptera: Simuliidae) from Thailand and its phylogenetic relationships with related species. J. Med. Entomol. 56: 86-94.
  • Tangkawanit, U. 2008. Cytogenetics of the Simulium tuberosum group (Diptera: Simuliidae) in Thailand. Ph. D. dissertation. Mahidol University, Bangkok, Thailand.
  • Tangkawanit, U., C. Kuvangkadilok, V. Baimai, and P. H. Adler. 2009. Cytosystematics of the Simulium tuberosum group (Diptera: Simuliidae) in Thailand. Zool. J. Linn. Soc. 155: 289-315.
  • Tangkawanit, U., K. Wongpakam, and P. Pramual. 2018. A new black fly (Diptera: Simuliidae) species of the subgenus Asiosimulium Takaoka & Choochote from Thailand. Zootaxa 4388: 111-122.
  • Thaijarern, J., P. Pramual, and P. H. Adler. 2018. Limited differentiation among black flies in the Simulium multistriatum species group (Diptera: Simuliidae) in Thailand: cryptic species, homosequential species, and homosequential cryptic species. Zool. J. Linn. Soc. 184: 1024-1054.
  • Vavra,J., and A.H.Undeen.1981.Microsporidia (Microspora:Microsporida) from Newfoundland blackflies (Diptera:Simuliidae).Can.J. Zool.59:1431-1446.
  • Vannote, R. L., and B.W.Sweeney. 1980. Geographic analysis of thermal equilibria: a conceptual model for evaluating the effect of natural and modified thermal regimes on aquatic insect communities. Am. Nat. 115: 667-695.