Craterellus cornucopioides Edible Mushroom as Source of Biologically Active Compounds

Here we determined the phenolic compounds and antioxidant, antimicrobial, genotoxic, and anticancer potential of edible mushrooms Craterellus cornucopioides. The phytochemical analysis was evaluated using high-performance liquid chromatography. Antioxidant activity was evaluated by free radical scavenging, superoxide anion scavenging, and reducing power. Craterellus cornucopioides extract had potent antioxidant activity. Further, the antimicrobial potential was determined by a microdilution method, where minimum inhibitory concentration values ranged from 0.1 to 10 mg/mL. Genotoxic potential was determined by cytokinesis block micronucleus test. The separate treatment did not show genotoxic effect, whereas the combined treatment with mitomycin C significantly reduced the micronuclei frequency in a dose-dependent manner. The highest concentration significantly reduced nuclear division index in comparison to untreated human peripheral blood lymphocytes, whereas in combined treatment, the extract did not significantly affect this parameter. Finally, the cytotoxic activity was tested using microculture tetrazolium test where measured IC₅₀ values ranged from 65.5 to 131.7 μg/mL.