GENERAL INFORMATION 1. Title of Dataset: Screening and microsatellite data for Varroa infesting resistant honey bee pupae 2. Author Information A. Principal Investigator Contact Information Name: Benjamin H. Conlon Institution: University of Copenhagen Address: Universitetsparken 15, 2100 Copenhagen E, Denmark Email: benjamin.conlon@bio.ku.dk B. Co-investigator Contact Information Name: Chedly Kastally Institution: University of Oulu Address: University of Oulu, Finland Email: chedly.kastally@oulu.fi C. Co-investigator Contact Information Name: Jarkko Routtu Institution: Martin-Luther-University Halle-Wittenberg Address: Hoher Weg 4, 06099, Halle (Saale) Germany Email: Jarkko.routtu@zoologie.uni-halle.de 3. Date of data collection : 2017 4. Geographic location of data collection : Near Villemur-sur-Tarn, Haute Garonne, France. 5. Information about funding sources that supported the collection of the data: Deutsche Forschungsgemeinschaft (RO 5121/1-1) SHARING/ACCESS INFORMATION 1. Links to publications that cite or use the data: https://doi.org/10.1002/ece3.6506 2. Recommended citation for this dataset: Conlon et al 2020: https://doi.org/10.1002/ece3.6506 DATA & FILE OVERVIEW File List: i. Screening_cell-by-cell.csv Per-cell Varroa infestation levels for 4 honey bee colonies ii. Microsatellites.xlsx Microsatellite data generated for Varroa collected from pupal cells. METHODOLOGICAL INFORMATION Methods are described in Conlon et al 2020 (https://doi.org/10.1002/ece3.6506) DATA-SPECIFIC INFORMATION FOR: Screening_cell-by-cell.csv 1. Number of variables: 8 2. Number of cases/rows: 173 3. Variable List: i. Colony: Colony of origin for pupal cell ii. Mothers: Total number of Varroa mothers in pupal cell iii. Dead: Total number of dead mothers in pupal cell iv. Sons: Total number of Varroa sons in pupal cell v. Daughters: Number of daughter mites identified vi. Successful: Production of at least 1 son and 1 daughter by infesting Varroa mothers vii. Infested: Cell infested by 1 or multiple Varroa mothers viii. Phenotype: Colony classified as "resistant" or "susceptible" to Varroa based on percentage of successfully-reproducing Varroa mothers (See Conlon et al 2020, https://doi.org/10.1002/ece3.6506) DATA-SPECIFIC INFORMATION FOR: Microsatellites.xlsx 1. Number of variables: 10 2. Number of cases/rows: 67 3. Variable List: i. Colony_ID: Colony of origin for pupal cell ii. Cell_ID: Cell of origin for Varroa mothers iii. VJ294_1: First microsatellite result for marker VJ294 iv. VJ294_2: Second microsatellite result for marker VJ294 v. VD307_1: First microsatellite result for marker VD307 vi. VD307_2: Second microsatellite result for marker VD307 vii. Vdes02_1: First microsatellite result for marker Vdes02 viii. Vdes02_2: Second microsatellite result for marker Vdes02 ix. n_unique_genotype_per_cell: Number of unique genotypes per cell x. successful_ie_presence_of_daughters: Did the Varroa in the cell reproduce successfully? i.e. Produced both sons and daughters 4. Missing data codes: 0 = no microsatellite data