This ALB_DORMANCY_README.txt file was generated on 2020-12-14 by ALEX S TORSON ################################################################################################# GENERAL INFORMATION 1. Title of Dataset: Dormancy in laboratory-reared Asian longhorned beetles, Anoplophora glabripennis 2. Author Information A. Corresponding Authors Contact Information Name: Alex S. Torson Institution: University of Western Ontario Address: 1151 Richmond St. London, ON, N6A 3K7, Canada Email: atorson@uwo.ca B. Principal Investigator Contact Information Name: Brent J. Sinclair Institution: University of Western Ontario Address: 1151 Richmond St. London, ON, N6A 3K7, Canada Email: bsincla7@uwo.ca C. Associate or Co-investigator Contact Information Name: Amanda D. Roe Institution: Great Lakes Forestry Centre, Natural Resources Canada Address: Sault Ste. Marie, Ontario P6A 2E5, Canada Email: amanda.roe@canada.ca 3. Date of data collection (YYY-MM-DD): 2016-12-16 - 2019-11-21 4. Geographic location of data collection: Great Lakes Forestry Centre Insect Production and Quarentine Laboratory - Sault Ste. Marie, ON, Canada 5. Information about funding sources that supported the collection of the data: This work was supported by Genome Canada, Genome British Columbia, and Genome Quebec [LSARP 10106; Genome Canada] grant for the Biosurveillance of Alien Forest Enemies (BioSAFE) project. ################################################################################################# SHARING/ACCESS INFORMATION 1. Licenses/restrictions placed on the data: NA 2. Links to publications that cite or use the data: https://doi.org/10.1016/j.jinsphys.2020.104179 3. Links to other publicly accessible locations of the data: NA 4. Links/relationships to ancillary data sets: NA 5. Was data derived from another source? No 6. Recommended citation for this dataset: See: https://doi.org/10.1016/j.jinsphys.2020.104179 ################################################################################################# DATA & FILE OVERVIEW 1. File List: ALB_Overwintering_Respirometry.csv ALB_Dormancy_Thermal_Sensitivity.csv ALB_Dormancy_Suspended_Development.csv ALB_Dormancy_Protein_Content.csv ALB_Dormancy_Water_Content.csv ALB_Dormancy_Carbohydrate_Content.csv ALB_Dormancy_Lipid_Content.csv 2. Relationship between files, if important: NA 3. Additional related data collected that was not included in the current data package: NA 4. Are there multiple versions of the dataset? yes/no No ################################################################################################# METHODOLOGICAL INFORMATION 1. Description of methods used for collection/generation of data: Please see publication for methods description or contact corresponding author for protocols. 2. Methods for processing the data: Raw data were processed as described in the associated publication. 3. Instrument- or software-specific information needed to interpret the data: Please see R scripts included with the associated publication. 4. Standards and calibration information, if appropriate: See publication. 5. Environmental/experimental conditions: See publication. 6. Describe any quality-assurance procedures performed on the data: See publication. 7. People involved with sample collection, processing, analysis and/or submission: See publication. ################################################################################################# DATA-SPECIFIC INFORMATION FOR: ALB_Overwintering_Respirometry.csv 1. Number of variables: 20 2. Number of cases/rows: 78 3. Variable List: "Individual_Number" - Unique code for each A. glabripennis individual "Population" - Source of quarantine population "Substrate" - Substrate that the egg was laid on (cheesecloth or log bolt) "Mating_Number" - Unique code for mating pair that produced the egg "Log_Number" - Unique code for which log (from a mating pair) the egg was laid on "Hatch_Date" - Date that the egg hatched "Diapause_Date" - Date that the individual was put in chilling (approx. 10 weeks post-hatch) "Time_Point" - Larval age as weeks-post-hatch "Mass_Before" - Mass (g) before measurement "Mass_After" - Mass (g) after measurement "Chamber_Number" - Chamber of respirometer multiplexer (1-8) "Run_Date" - Date that the measurements were made "Run_File_Name" - Associated Expedata file "mean_VCO2" - Average VCO2 (ml/min) within measurement range "stddev_VCO2" - Standard deviation of VCO2 (ml/min) within measurement range "n_VCO2_1" - Number of samples (seconds) selected for analysis "startsample_VCO2" - First sample of analysis region "endsample_VCO2" - Last sample of analysis region "totalsamples_VCO2" - Total number of samples (seconds) recorded "Notes" - Any additional notes about sample/run/measurement 4. Missing data codes: NA 5. Specialized formats or other abbreviations used: NA ################################################################################################# DATA-SPECIFIC INFORMATION FOR: ALB_Dormancy_Thermal_Sensitivity.csv 1. Number of variables: 25 2. Number of cases/rows: 192 3. Variable List: "Individual_Number" - Unique code for each A. glabripennis individual "Population" - Source of quarantine population "Substrate" - Substrate that the egg was laid on (cheesecloth or log bolt) "Group_Temp" - Treatment group "Storage_Temp" - Temperature that each treatment group was stored at during respective time point "Run_Temp" - Temperature (degC) at which respirometry measurements were made "Mating_Number" - Unique code for mating pair that produced the egg "Log_Number" - Unique code for which log (from a mating pair) the egg was laid on "Hatch_Date" - Date that the egg hatched "Diapause_Date" - Date that the individual was put in chilling (approx. 10 weeks post-hatch) "Time_Point" - Larval age as weeks-post-hatch "Mass_Before" - Mass (g) before measurement "Mass_After" - Mass (g) after measurement "Chamber_Number" - Chamber of respirometer multiplexer (1-8) "Run_Date" - Date that the measurements were made "Run_File_Name" - Associated Expedata file "mean_VCO2" - Average VCO2 (ml/min) within measurement range "stddev_VCO2" - Standard deviation of VCO2 (ml/min) within measurement range "n_VCO2_1" - Number of samples (seconds) selected for analysis "startsample_VCO2" - First sample of analysis region "endsample_VCO2" - Last sample of analysis region "totalsamples_VCO2" - Total number of samples (seconds) recorded "Pattern" - Observed gas exchange pattern ("Cyclic" or "Continuous". "Trash" removed from analysis) "Notes" - Any additional notes about sample/run/measurement 4. Missing data codes: NA 5. Specialized formats or other abbreviations used: NA ################################################################################################# DATA-SPECIFIC INFORMATION FOR: ALB_Dormancy_Suspended_Development.csv 1. Number of variables: 12 2. Number of cases/rows: 30 3. Variable List: "Population" - Source of quarantine population "Individual_Number" - Unique code for each A. glabripennis individual "Mating_Number" - Unique code for mating pair that produced the egg "Log_Number" - Unique code for which log (from a mating pair) the egg was laid on "Hatch_Date" - Date that the egg hatched "Chill_Start_Date" - Date that the larva was placed into chilling (6degC) "Time_Point" - Number of weeks that larva were chilled prior to removal "Chill_End_Date" - Date that larva was removed from chilling "Start_Mass" - Larva mass at ten weeks (prior to chilling) "Feeding_Date" - Date that larva first fed after removal from chilling "Pupation_Date" - Date that larva pupated "Pupal_Mass" - Mass of pupa "Days_To_Feeding" - Number of days after removal from chilling until larva fed "Days_To_Pupation" - Number of days after removal from chilling until larva pupated "Return_To_Chill" - Date that larva was placed back in chilling (if necessary) "Second_Post_Chill" - Date of removal from second round of chilling (if necessary) "Notes" - Any additional notes about sample/run/measurement 4. Missing data codes: NA 5. Specialized formats or other abbreviations used: NA ################################################################################################# DATA-SPECIFIC INFORMATION FOR: ALB_Dormancy_Protein_Content.csv 1. Number of variables: 13 2. Number of cases/rows: 20 3. Variable List: "Time_Point_Weeks" - Larval age as weeks-post-hatch "Individual_Number" - Unique code for each A. glabripennis individual "Date_Collected" - Date that larva was dissected "Substrate" - Substrate that the egg was laid on (cheesecloth or log bolt) "Colony" - Source of quarantine population "Individual_Code" - Unique code for each A. glabripennis individual "Mating_Number" - Unique code for mating pair that produced the egg "Log_Number" - Unique code for which log (from a mating pair) the egg was laid on "Hatch_Date" - Date that the egg hatched "Chill_Start" - Date that larva was transferred to chilling "Chill_Stop"- Date that larva was removed from chilling "Tissue_Mass" - Mass (mg) of dry tissue used for measurement "Protein_Concentration" - protein concentration (ug protein/mg tissue) 4. Missing data codes: NA 5. Specialized formats or other abbreviations used: NA ################################################################################################# DATA-SPECIFIC INFORMATION FOR: ALB_Dormancy_Water_Content.csv 1. Number of variables: 39 2. Number of cases/rows: 20 3. Variable List: "Time_Point_Weeks" - Larval age as weeks-post-hatch "Individual_Number" - Unique code for each A. glabripennis individual "Date_Collected" - Date that larva was dissected "Substrate" - Substrate that the egg was laid on (cheesecloth or log bolt) "Colony" - Source of quarantine population "Individual_Code" - Unique code for each A. glabripennis individual "Mating_Number" - Unique code for mating pair that produced the egg "Log_Number" - Unique code for which log (from a mating pair) the egg was laid on "Hatch_Date" - Date that the egg hatched "Chill_Start" - Date that larva was transferred to chilling "Chill_Stop"- Date that larva was removed from chilling "Soo_Mass" - Larval mass (measured prior to shipment to UWO) "Tube_Mass" - Mass of clean 1.5mL microcentrifuge tube "Tube_Fresh_Mass" - Mass of microcentrifuge tube containing larva "FM" - Larval fresh mass ("Tube_Fresh_Mass" minus "Tube_Mass") "Date_Weighed" - Date of initial mass measurement "Dry_Mass_Tube" - Mass of new microcentrifuge tube with dried larvae (day 1) "DM" - Dry mass of larva day 1 ("Dry_Mass_Tube" - "Tube_Mass") "Date_Weighed.1" - Date of dry mass measurement "Dry_Mass_Tube.1" - Mass of new microcentrifuge tube with dried larvae (day 2) "DM.1" - Dry mass of larva day 2 ("Dry_Mass_Tube.1" - "Tube_Mass") "Change_in_DM" - Change in dry mass from previous measurement "percent_water_content" - Percent water content (FM - DM.1/FM * 100) "Date_Weighed.2" - Date of dry mass measurement "Dry_Mass_Tube.2" - Mass of new microcentrifuge tube with dried larvae (day 3) "DM.2" - Dry mass of larva day 3 ("Dry_Mass_Tube.2" - "Tube_Mass") "Change_in_DM.1" - Change in dry mass from previous measurement "percent_water_content.1"- Percent water content (FM - DM.2/FM * 100) "Date_Weighed.3" - Date of dry mass measurement "Dry_Mass_Tube.3" - Mass of new microcentrifuge tube with dried larvae (day 4) "DM.3" - Dry mass of larva day 4 ("Dry_Mass_Tube.3" - "Tube_Mass") "Change_in_DM.2" - Change in dry mass from previous measurement "percent_water_content.2"- Percent water content (FM - DM.3/FM * 100) "Date_Weighed.4" - Date of dry mass measurement "Dry_Mass_Tube.4" - Mass of new microcentrifuge tube with dried larvae (day 5) "DM.4" - Dry mass of larva day 5 ("Dry_Mass_Tube.4" - "Tube_Mass") "Change_in_DM.3" - Change in dry mass from previous measurement "percent_water_content.3"- Percent water content (FM - DM.4/FM * 100) "Date_Weighed.5" - Date of dry mass measurement 4. Missing data codes: NA 5. Specialized formats or other abbreviations used: NA ################################################################################################# DATA-SPECIFIC INFORMATION FOR: ALB_Dormancy_Carbohydrate_Content.csv 1. Number of variables: 13 2. Number of cases/rows: 20 3. Variable List: "Time_Point_Weeks" - Larval age as weeks-post-hatch "Individual_Number" - Unique code for each A. glabripennis individual "Date_Collected" - Date that larva was dissected "Substrate" - Substrate that the egg was laid on (cheesecloth or log bolt) "Colony" - Source of quarantine population "Individual_Code" - Unique code for each A. glabripennis individual "Mating_Number" - Unique code for mating pair that produced the egg "Log_Number" - Unique code for which log (from a mating pair) the egg was laid on "Hatch_Date" - Date that the egg hatched "Chill_Start" - Date that larva was transferred to chilling "Chill_Stop"- Date that larva was removed from chilling "Tissue_Mass" - Mass (mg) of dry tissue used for measurement "Carbohydrate_Concentration" - carbohydrate concentration (ug carb/mg tissue) 4. Missing data codes: NA 5. Specialized formats or other abbreviations used: NA ################################################################################################# DATA-SPECIFIC INFORMATION FOR: ALB_Dormancy_Lipid_Content.csv 1. Number of variables: 30 2. Number of cases/rows: 20 3. Variable List: "Time_Point_Weeks" - Larval age as weeks-post-hatch "Individual_Number" - Unique code for each A. glabripennis individual "Date_Collected" - Date that larva was dissected "Substrate" - Substrate that the egg was laid on (cheesecloth or log bolt) "Colony" - Source of quarantine population "Individual_Code" - Unique code for each A. glabripennis individual "Mating_Number" - Unique code for mating pair that produced the egg "Log_Number" - Unique code for which log (from a mating pair) the egg was laid on "Hatch_Date" - Date that the egg hatched "Chill_Start" - Date that larva was transferred to chilling "Chill_Stop"- Date that larva was removed from chilling "Tube_mass" - Mass of clean 1.5mL microcentrifuge tube "Tube_Tissue_Mass" - Mass of microcentrifuge tube with larva "Fresh_mass" - Fresh mass of larva "Dry_Mass" - Dry mass of larva "Dry_Tissue_Mass" - Mass of dry tissue as input for Anthrone assay "Date_Weighed" - Date that "Dry_Tissue_Mass" was weighed "Tube_Tissue_Mass.1" - Tube plus tissue mass after first wash "Lipid_Free_Dry_Tissue_Mass" - LFDM after first wash "Percent_Lipid_Content" - Percent lipid content after first wash "Date_Weighed.1" - Date of weight measurement after first wash "Tube_Tissue_Mass.2" - Tube plus tissue mass after second wash "Lipid_Free_Dry_Tissue_Mass.1" - LFDM after second wash "Percent_Lipid_Content.1" - Percent lipid content after second wash "Date_Weighed.2" - Date of weight measurement after second wash "Tube_Tissue_Mass.3" - Tube plus tissue mass after second wash "Lipid_Free_Dry_Tissue_Mass.2" - LFDM after third wash "lipid_per_dry_mass" - Lipid content (mg) per gram of dried mass "Percent_Lipid_Content.2" - Percent lipid content "Date_Weighed.3" - Date of weight measurement after third wash 4. Missing data codes: NA 5. Specialized formats or other abbreviations used: NA