This readme file explains what each dataset uploaded contains and how the data were analyzed.

Please refer to the methods section in the manuscript for how the sequence data was generated and filtered to produce the following files.

Salignum_cp_2020-06.csv is a file that includes individual IDs, population IDs, latitude, longitude, and presence/absence (1/0) of SNPs sequenced from chloroplast DNA of L. Salignum. This format is compatible with the R package “gstudio” 

Salignum_nu_2020-06.txt is a fast file format of the SNPs identified for the nuclear DNA of L salignum, including individual, population, and chromosome IDs in the heading. These data were used to calculate FST and other demographic metrics.

LS-nuDNA_for_cGD.csv and Salignum2.csv are comma separated files in studio format. The former contains information for the nuclear DNA and the latter is for chloroplast DNA. Each of the columns after the site/individual information represents a SNP site to be used for cGD calculation in R.

LocationsSA1.csv has the locale information for each of the sampled sites, used to generate sampling maps on rasters using R.

SAcpFstFull.txt and nu_FST.txt are matrices that contain the FST values for both chloroplast and nu DNA respectively. Pairwise FST values were calculated in R and these matrices were generated. Then these matrices were used as both Figures in Support text and for data usage in landscape analyses in R.

RodneyIBRb.R is an R script that has all of the code that was used in the landscape genetic analyses, as mentioned in the Methods section of the manuscript.

SalignumPCA.R is an R file that has the standard scripts that we used to generate PCAs in R.