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Published December 15, 2020 | Version v1
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Neuronal cell labelled with anti PSD95 and homer antibodies - sample image for software testing of Easy SODA

  • 1. INSERM


Hippocampal neurons in culture seeded on glass coverslip. This 16 bits confocal picture of hippocampal neuron in culture has been taken at 63x (1024x1024 pixels - pixel size 92,45 nm) in sequential mode with two channels : one dedicated to the PSD95 protein, and the other one to Homer protein. Those protein are expressed at glutamatergic post-synaptic sites and can be found close within Post Synaptic Density. This is a good sample to test colocalization and association analysis. The homer channel can be used both to infere neuronal shape thanks to hk-means segmentation (, or to detect clusters thanks to wavelet segmentation ( This sample image can be used with Easy SODA protocol (Standard Object Distance Analysis) on Icy software ( to detect association between PSD95 and homer clusters.



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Software: 10.5281/zenodo.4317783 (DOI)