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Published December 15, 2020 | Version v1
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Neuronal cell labelled with anti PSD95 and homer antibodies - sample image for software testing of Easy SODA

  • 1. INSERM

Description

Hippocampal neurons in culture seeded on glass coverslip. This 16 bits confocal picture of hippocampal neuron in culture has been taken at 63x (1024x1024 pixels - pixel size 92,45 nm) in sequential mode with two channels : one dedicated to the PSD95 protein, and the other one to Homer protein. Those protein are expressed at glutamatergic post-synaptic sites and can be found close within Post Synaptic Density. This is a good sample to test colocalization and association analysis. The homer channel can be used both to infere neuronal shape thanks to hk-means segmentation (http://icy.bioimageanalysis.org/protocol/easy-cell-shape-with-hk-means/), or to detect clusters thanks to wavelet segmentation (http://icy.bioimageanalysis.org/protocol/rainbow-spot-detector-scale-finder/). This sample image can be used with Easy SODA protocol (Standard Object Distance Analysis) on Icy software (http://icy.bioimageanalysis.org/protocol/easy-soda-2-colors-1-image/) to detect association between PSD95 and homer clusters.

Files

A_preview_of_Easy_SODA_PSD95_Homer_.tif

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Additional details

Related works

Is supplemented by
Software: 10.5281/zenodo.4317783 (DOI)