Published November 18, 2020 | Version v1
Dataset Open

Competition for pollination and isolation from mates differentially impact four stages of pollination in a model grassland perennial

  • 1. Northwestern University
  • 2. Tel Aviv University
  • 3. University of California, Davis
  • 4. Utah State University
  • 5. Chicago Botanic Garden*
  • 6. University of Pittsburgh
  • 7. Martin Luther College

Description

1. Species that persist in small populations isolated by habitat destruction may experience reproductive failure. Self-incompatible plants face dual threats of mate-limitation and competition with co-flowering plants for pollination services. Such competition may lower pollinator visitation, increase heterospecific pollen transfer, and reduce the likelihood that a visit results in successful pollination.

2. To understand how isolation from mates and competition with co-flowering species contribute to reproductive failure in fragmented habitat, we conducted an observational study of a tallgrass prairie perennial, Echinacea angustifolia. We quantified the isolation of focal individuals from mates, characterized species richness and counted inflorescences within 1m radius, observed pollinator visitation, collected pollinators, quantified pollen loads on pollinators and on Echinacea stigmas, and measured pollination success. Throughout the season, we sampled 223 focal plants.

3. We present evidence that both co-flowering species and isolation from mates substantially limit reproduction in Echinacea. As the flowering season progressed, the probability of pollinator visitation to focal plants decreased and evidence for pollen-limited reproduction increased. Pollinators were most likely to visit Echinacea plants from low-richness floral neighbourhoods with close potential mates, or plants from high-richness neighbourhoods with distant potential mates. Frequent visitation only increased pollination success in the former case, likely because Echinacea in high-richness floral neighbourhoods received low-quality visits.

4. Synthesis: In Echinacea, reproduction was limited by isolation from potential mates and the richness of co-flowering species. These aspects of the floral neighbourhood influenced pollinator visitation and pollination success, although conditions that predicted high visitation did not always lead to high pollination success. These results reveal how habitat modification and destruction, which influence floral neighbourhood and isolation from conspecific mates, can differentially affect various stages of reproductive biology in self-incompatible plants. Our results suggest that prairie conservation and restoration efforts that promote patches of greater floral diversity may improve reproductive outcomes in fragmented habitats.

Notes

Please refer to the readMe.txt file for metadata and descriptions of all included files.

1. ff.csv
2. bb.csv
3. ppCon.csv
4. ppHet.csv
5. ssCon.csv
6. ssHet.csv
7. rr.csv
8. pollinatorCommunity.R
9. pollinatorVisitationAnalysis.R
10. pollenOnPollinatorsAnalysis.R
11. pollenOnStylesAnalysis.R
12. stylePersistenceAnalysis.R
13. supplementalFigures.R
 

Funding provided by: National Science Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001
Award Number: 0544970, 0545072, 0648972, 1355187, 1555997, and 1557075

Files

bb.csv

Files (247.3 kB)

Name Size Download all
md5:a4fc1135d5c538d3175105279f1d5794
51.9 kB Preview Download
md5:60a3b02160acb29a82283ea1d7add456
43.5 kB Preview Download
md5:394295fc01abcc20a79c94c2de6872ba
22.9 kB Download
md5:7e80312d361e1720b98bbd54a946a713
29.0 kB Download
md5:1e1bc5389a1a419db446e62fec6c2363
11.2 kB Download
md5:fb7f3926b8860674be2736b2e3ec592f
15.4 kB Download
md5:61af06b60dd51814bef261ec9f911128
8.5 kB Preview Download
md5:318064396ccf6ac943bb4357d17c4a4a
4.0 kB Preview Download
md5:7e0d0663902d00401572bd7dd891fe7b
9.5 kB Preview Download
md5:eca750e37f14e245144570e8738c36c1
2.3 kB Preview Download
md5:74f7aae74d35e8a7ad4883676b86dca5
10.6 kB Preview Download
md5:25f1bb3ec666b01075ae825e94493ef7
10.4 kB Preview Download
md5:456086144c38f7612604d4de8a0244ee
15.4 kB Download
md5:1846f558adfca52565272ffbe69a1af6
12.7 kB Download