* Block Type = 96well * Chemistry = SYBR_GREEN * Experiment File Name = \\172.16.2.119\LabBSBM\Bio Molecolare\REAL TIME\miRNA\2017 pannelli\Results\Panel 1 A mirna circolanti.eds * Experiment Run End Time = Not Started * Instrument Type = steponeplus * Passive Reference = ROX [Results] Well Sample Name Target Name Task Reporter Quencher Cт Cт Mean Cт SD Quantity Quantity Mean Quantity SD Automatic Ct Threshold Ct Threshold Automatic Baseline Baseline Start Baseline End Tm1 Tm2 Tm3 Comments NOAMP EXPFAIL THOLDFAIL MTP A1 Sample 1 hsa-miR-652-3p UNKNOWN SYBR None 36.9271 36.9271 true 0.9633 true 3 29 70.989 N N N N A2 Sample 1 UniSp3 IPC UNKNOWN SYBR None 21.881 21.8731 0.0893 true 1.37 true 3 14 70.393 N N N N A3 Sample 1 hsa-miR-221-3p UNKNOWN SYBR None 35.9577 35.9577 true 1.0346 true 3 29 68.3157 N N N N A4 Sample 1 let-7f-5p UNKNOWN SYBR None 35.9644 35.9644 true 1.3196 true 3 28 68.4647 N N N N A5 Sample 1 hsa-miR-27b-3p UNKNOWN SYBR None 33.9871 33.9871 true 1.5315 true 3 25 69.8 N N N N A6 Sample 1 hsa-miR-374b-5p UNKNOWN SYBR None 35.9234 35.9234 true 1.0831 true 3 29 69.2041 N N N N A7 Sample 1 hsa-miR-93-5p UNKNOWN SYBR None 33.9489 33.9489 true 1.4617 true 3 26 71.5865 N N N N A8 Sample 1 hsa-miR-200a-3p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 66.3718 87.2309 N Y Y Y A9 Sample 1 UniSp2 UNKNOWN SYBR None 29.9353 29.9353 true 1.4099 true 3 21 70.3966 N N N N A10 Sample 1 hsa-miR-484 UNKNOWN SYBR None 36.9238 36.9238 true 1.1378 true 3 29 69.9497 N N N N A11 Sample 1 hsa-miR-181a-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 65.9265 84.5516 N Y Y Y A12 Sample 1 hsa-miR-106a-5p UNKNOWN SYBR None 34.9479 34.9479 true 2.7971 true 3 26 71.7375 N N N N B1 Sample 1 hsa-miR-145-5p UNKNOWN SYBR None 35.9536 35.9536 true 0.6857 true 3 28 68.9031 N N N N B2 Sample 1 UniSp3 IPC UNKNOWN SYBR None 21.7801 21.8731 0.0893 true 1.37 true 3 14 70.393 N N N N B3 Sample 1 hsa-miR-486-5p UNKNOWN SYBR None 29.9178 29.9178 true 1.7651 true 3 22 72.9342 N N N N B4 Sample 1 hsa-miR-26a-5p UNKNOWN SYBR None 34.9423 34.9423 true 1.1639 true 3 27 70.4015 N N N N B5 Sample 1 hsa-miR-143-3p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 64.4368 Y Y Y N B6 Sample 1 hsa-miR-30a-5p UNKNOWN SYBR None 37.1541 37.1541 true 0.0401 true 3 35 70.3959 N N N N B7 Sample 1 hsa-miR-222-3p UNKNOWN SYBR None 35.9428 35.9428 true 0.6995 true 3 29 68.6067 N N N N B8 Sample 1 hsa-miR-342-3p UNKNOWN SYBR None 36.9196 36.9196 true 1.2862 true 3 29 69.6496 N N N N B9 Sample 1 UniSP4 UNKNOWN SYBR None 35.914 35.914 true 0.982 true 3 29 69.056 N N N N B10 Sample 1 hsa-miR-324-5p UNKNOWN SYBR None 37.1093 37.1093 true 0.1115 true 3 33 72.482 N N N N B11 Sample 1 hsa-miR-185-5p UNKNOWN SYBR None 33.9501 33.9501 true 2.7463 true 3 25 70.8435 N N N N B12 Sample 1 hsa-let-7e-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 68.6085 78.2935 N Y Y Y C1 Sample 1 hsa-miR-139-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 65.1782 68.0091 85.5906 N Y Y Y C2 Sample 1 UniSp3 IPC UNKNOWN SYBR None 21.9582 21.8731 0.0893 true 1.37 true 3 13 70.393 N N N N C3 Sample 1 hsa-miR-451a UNKNOWN SYBR None 30.9523 30.9523 true 1.9485 true 3 22 69.6566 N N N N C4 Sample 1 hsa-let-7a-5p UNKNOWN SYBR None 35.9929 35.9929 true 1.62 true 3 28 68.7627 N N N N C5 Sample 1 hsa-miR-128-3p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 64.4368 N Y Y N C6 Sample 1 hsa-miR-125a-5p UNKNOWN SYBR None 35.9551 35.9551 true 0.4563 true 3 30 68.9062 N N N N C7 Sample 1 hsa-miR-485-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 63.8389 N Y Y N C8 Sample 1 hsa-let-7b-3p UNKNOWN SYBR None 37.1288 37.1288 true 0.0336 true 3 35 70.2456 N N N N C9 Sample 1 UniSp5 UNKNOWN SYBR None Undetermined true 0.2 true 3 39 64.1404 Y Y Y N C10 Sample 1 hsa-miR-25-3p UNKNOWN SYBR None 31.9372 31.9372 true 2.7052 true 3 23 70.0987 N N N N C11 Sample 1 hsa-miR-375 UNKNOWN SYBR None 36.9305 36.9305 true 0.6902 true 3 30 70.6945 N N N N C12 Sample 1 hsa-miR-33a-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 68.0125 87.8296 90.3626 N Y Y Y D1 Sample 1 hsa-miR-16-5p UNKNOWN SYBR None 25.94 25.94 true 1.0347 true 3 17 70.84 N N N N D2 Sample 1 hsa-let-7b-5p UNKNOWN SYBR None 31.975 31.975 true 1.3243 true 3 24 70.244 N N N N D3 Sample 1 hsa-miR-152-3p UNKNOWN SYBR None 35.9528 35.9528 true 0.7278 true 3 29 69.3586 N N N N D4 Sample 1 hsa-miR-30c-5p UNKNOWN SYBR None 35.9301 35.9301 true 1.3955 true 3 27 70.1035 N N N N D5 Sample 1 hsa-miR-197-3p UNKNOWN SYBR None 35.9646 35.9646 true 0.9137 true 3 30 68.9062 N N N N D6 Sample 1 hsa-miR-99b-5p UNKNOWN SYBR None 35.9303 35.9303 true 0.8972 true 3 28 69.2041 N N N N D7 Sample 1 hsa-miR-30e-5p UNKNOWN SYBR None 35.9666 35.9666 true 1.5446 true 3 28 70.6926 N N N N D8 Sample 1 hsa-miR-146a-5p UNKNOWN SYBR None 35.917 35.917 true 0.7199 true 3 29 69.5006 N N N N D9 Sample 1 cel-miR-39-3p UNKNOWN SYBR None 28.9987 28.9987 true 0.9413 true 3 21 69.3539 N N N N D10 Sample 1 hsa-miR-424-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 65.3321 N Y Y N D11 Sample 1 UniSp6 UNKNOWN SYBR None 19.9217 19.9217 true 1.1697 true 3 11 72.7805 N N N N D12 Sample 1 hsa-miR-107 UNKNOWN SYBR None 37.0769 37.0769 true 0.1807 true 3 32 68.7575 N N N N E1 Sample 1 hsa-miR-148b-3p UNKNOWN SYBR None 36.9521 36.9521 true 0.7077 true 3 30 70.393 N N N N E2 Sample 1 hsa-miR-20a-5p UNKNOWN SYBR None 33.9448 33.9448 true 1.1164 true 3 26 70.989 N N N N E3 Sample 1 hsa-miR-17-5p UNKNOWN SYBR None 37.0938 37.0938 true 0.1798 true 3 32 72.6362 N N N N E4 Sample 1 hsa-miR-30d-5p UNKNOWN SYBR None 33.9069 33.9069 true 1.706 true 3 26 72.3382 N N N N E5 Sample 1 hsa-miR-186-5p UNKNOWN SYBR None 37.1153 37.1153 true 0.0712 true 3 33 68.9062 N N N N E6 Sample 1 let-7i-5p UNKNOWN SYBR None 35.977 35.977 true 1.0849 true 3 29 69.2041 N N N N E7 Sample 1 hsa-miR-26b-5p UNKNOWN SYBR None 35.9547 35.9547 true 1.2487 true 3 28 68.7557 N N N N E8 Sample 1 hsa-miR-34a-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 64.8818 Y Y Y N E9 Sample 1 hsa-miR-320b UNKNOWN SYBR None 32.9974 32.9974 true 1.3863 true 3 24 70.8435 N N N N E10 Sample 1 hsa-miR-590-5p UNKNOWN SYBR None 36.9724 36.9724 true 0.6196 true 3 31 68.7581 N N N N E11 Sample 1 hsa-miR-191-5p UNKNOWN SYBR None 33.9358 33.9358 true 1.6947 true 3 25 70.0985 N N N N E12 Sample 1 hsa-miR-99a-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 76.3565 65.1815 68.1615 N Y Y Y F1 Sample 1 hsa-miR-301a-3p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 64.1352 N Y Y N F2 Sample 1 hsa-miR-151a-5p UNKNOWN SYBR None 35.9867 35.9867 true 1.2639 true 3 28 69.3501 N N N N F3 Sample 1 hsa-miR-122-5p UNKNOWN SYBR None 36.9333 36.9333 true 0.9867 true 3 29 69.8055 N N N N F4 Sample 1 hsa-miR-423-5p UNKNOWN SYBR None 34.9748 34.9748 true 1.5644 true 3 27 70.4015 N N N N F5 Sample 1 hsa-miR-101-3p UNKNOWN SYBR None 34.9612 34.9612 true 0.8384 true 3 27 70.3959 N N N N F6 Sample 1 hsa-miR-365a-3p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 64.8837 68.1613 79.6326 N Y Y Y F7 Sample 1 hsa-miR-23a-3p UNKNOWN SYBR None 30.9646 30.9646 true 1.7857 true 3 22 70.6926 N N N N F8 Sample 1 hsa-miR-215-5p UNKNOWN SYBR None 37.1252 37.1252 true 0.0173 true 3 35 70.2456 N N N N F9 Sample 1 hsa-miR-320a UNKNOWN SYBR None 30.928 30.928 true 1.4894 true 3 23 72.482 N N N N F10 Sample 1 hsa-miR-338-3p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 64.7363 87.5265 90.5056 Y Y Y Y F11 Sample 1 hsa-miR-223-3p UNKNOWN SYBR None 28.9356 28.9356 true 1.6312 true 3 21 70.3965 N N N N F12 Sample 1 hsa-miR-103a-3p UNKNOWN SYBR None 34.9685 34.9685 true 1.4381 true 3 27 69.6515 N N N N G1 Sample 1 hsa-miR-331-3p UNKNOWN SYBR None 36.9499 36.9499 true 0.6 true 3 30 70.095 N N N N G2 Sample 1 hsa-miR-142-3p UNKNOWN SYBR None 36.0188 36.0188 true 1.1374 true 3 28 71.883 N N N N G3 Sample 1 hsa-let-7d-3p UNKNOWN SYBR None 35.9633 35.9633 true 1.6358 true 3 27 69.3586 N N N N G4 Sample 1 hsa-miR-126-5p UNKNOWN SYBR None 32.9368 32.9368 true 0.8787 true 3 25 71.1464 N N N N G5 Sample 1 hsa-miR-19a-3p UNKNOWN SYBR None 33.9162 33.9162 true 2.3458 true 3 25 70.3959 N N N N G6 Sample 1 hsa-miR-144-3p UNKNOWN SYBR None 34.8984 34.8984 true 0.7834 true 3 28 69.2041 N N N N G7 Sample 1 hsa-miR-126-3p UNKNOWN SYBR None 31.9383 31.9383 true 2.068 true 3 23 70.5436 N N N N G8 Sample 1 hsa-miR-148a-3p UNKNOWN SYBR None 37.1297 37.1297 true 0.0162 true 3 35 69.3516 N N N N G9 Sample 1 hsa-miR-10b-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 64.7363 Y Y Y N G10 Sample 1 hsa-miR-195-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 65.9279 Y Y Y N G11 Sample 1 hsa-miR-125b-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 64.7345 Y Y Y N G12 Sample 1 hsa-miR-192-5p UNKNOWN SYBR None 35.9237 35.9237 true 1.9688 true 3 28 70.9925 N N N N H1 Sample 1 hsa-miR-18b-5p UNKNOWN SYBR None 37.1028 37.1028 true 0.0003 true 3 35 70.393 N N N N H2 Sample 1 hsa-miR-335-5p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 88.2726 66.2211 68.6051 N Y Y Y H3 Sample 1 hsa-miR-320d UNKNOWN SYBR None 35.945 35.945 true 2.0394 true 3 27 70.9974 N N N N H4 Sample 1 hsa-let-7g-5p UNKNOWN SYBR None 34.9328 34.9328 true 1.3747 true 3 27 69.6566 N N N N H5 Sample 1 hsa-miR-22-3p UNKNOWN SYBR None 36.9669 36.9669 true 0.3235 true 3 31 69.2041 N N N N H6 Sample 1 hsa-miR-199a-3p UNKNOWN SYBR None 35.9381 35.9381 true 0.9318 true 3 29 69.2041 N N N N H7 Sample 1 hsa-miR-19b-3p UNKNOWN SYBR None 32.9664 32.9664 true 1.2944 true 3 25 69.2026 N N N N H8 Sample 1 hsa-miR-29a-3p UNKNOWN SYBR None Undetermined true 0.2 true 3 39 65.4778 87.8269 N Y Y Y H9 Sample 1 hsa-miR-150-5p UNKNOWN SYBR None 34.9649 34.9649 true 1.1942 true 3 27 71.4393 N N N N H10 Sample 1 hsa-miR-30b-5p UNKNOWN SYBR None 35.9788 35.9788 true 1.6849 true 3 27 68.6091 N N N N H11 Sample 1 hsa-miR-24-3p UNKNOWN SYBR None 31.9442 31.9442 true 1.0652 true 3 24 74.7175 N N N N H12 Sample 1 blank H2O UNKNOWN SYBR None Undetermined true 0.2 true 3 39 68.3105 70.9925 91.5546 N Y Y Y