Published December 31, 2017 | Version v1
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Chromosomal evidence for a new cryptic species of black fly in the Simulium praelargum complex (Diptera: Simuliidae) from West Bengal, India


Thapa, Sachin, Adler, Peter H., Chhetri, Shailika, Varma, Rakesh, Henry, Willie (2017): Chromosomal evidence for a new cryptic species of black fly in the Simulium praelargum complex (Diptera: Simuliidae) from West Bengal, India. Zootaxa 4244 (1): 137-144, DOI: 10.11646/zootaxa.4244.1.8



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  • Adler, P.H. (2005) Black flies, the Simuliidae. In Marquardt, W.C. (Ed.), Biology of Disease Uectors. 2nd Edition. Elsevier Academic Press, San Diego, CA, pp. 127‾140.
  • Adler, P.H. & Crosskey, R.W. (2015) Cytotaxonomy of the Simuliidae (Diptera): a systematic and bibliographic conspectus. Zootaxa, 3975 (1), 1‾139.
  • Adler, P.H. & Crosskey, R.W. (2016) World blackflies (Diptera: Simuliidae): a comprehensive revision of the taxonomic and geographical inventory. Available from: (accessed 20 February 2017)
  • Adler, P.H., Yadamsuren, O. & Procunier, W.S. (2016) Chromosomal translocations in black flies (Diptera: Simuliidae): facilitators of adaptive radiation? PLoS ONE, 11 (6), e0158272.
  • Bedo, D.G. (1976) Polytene chromosomes in pupal and adult black flies (Diptera: Simuliidae). Chromosoma, 57, 387‾396.
  • Brockhouse, C. (1985) Sibling species and sex chromosomes in Eusimulium vernum (Diptera: Simuliidae). Canaadian Jounal of Zool ogy, 63, 2145-2161.
  • Datta, M. (1975) A new black fly species (Simuliidae-Diptera) from the Darjeeling area, India. Proceedings of the Indian Academy of Science, 81 B (2), 67-74.
  • Dey, S.K. & Fumafartsok, T.W. (1984) Supernumerary chromosomes in two species of blackflies (Diptera: Simuliidae). Cell & Chromosome Research, 7, 28-30.
  • Dey, S.K., Henry, W. & Varma, R. (1993) The salivary gland chromosomes of the black fly Simulium (Simulium) singtamense (Diptera: Simuliidae). Cytologia, 58, 299‾304.
  • Hadi, U.K., Takaoka, H. & Aoki, C. (1996) Larval polytene chromosomes of three Japanese blackfly species (Diptera: Simuliidae). Medical Entomology & Zoology, 47, 47-54.
  • Henry, W., Dey, S.K. & Varma, R. (2009) The salivary gland chromosomes of the black fly Simulium (Simulium) dentatum (Diptera: Simuliidae). Zoological Science, 26, 216‾219.
  • Henry, W., Dey, S.K., Varma, R., Thapa, S. & Procunier, W.S. (2010) Polytene chromosomes of an Indian Himalayan black fly Simulium (Nevermannia) praelargum (Diptera: Simuliidae). Current Zoology, 56 (4), 437-444.
  • Hunter, F.F. & Connolly, V. (1986) A cytotaxonomic investigation of seven species in the Eusimulium vernum group (Diptera: Simuliidae). Canadian Journal of Zoology, 64, 296-311.
  • Kuvangkadilok, C., Phayuhasena, S. & Baimai, V. (1999) Population cytogenetic studies on Simulium feuerborni Edwards (Diptera: Simuliidae) from Northern Thailand. Genome, 42, 80-86.
  • Kuvangkadilok, C., Lualon, U. & Baimai, V. (2008) Cytotaxonomy of Simulium siamense Takaoka and Suzuki (Diptera: Simuliidae) in Thailand. Genome, 51, 972‾987.
  • Low, V.L., Takaoka, H., Pramual, P., Adler, P.H., Ya'cob, Z., Huang, Y.-T., Pham, X.D., Ramli, R., Chen, C.D., Wannaket, A. & Sofian-Azirun, M. (2016) Delineating taxonomic boundaries in the largest species complex of black flies (Simuliidae) in the Oriental Region. Scientific Reports, 6 (20346), 1‾8.
  • Otsuka, Y., Aoki, C., Saito, K., Hadi, U.K., Suzuki, H. & Takaoka, H. (2001) Phylogenetic analyses of a black fly subgenus Simulium (Nevermannia) based on mitochondrial 16S ribosomal RNA gene sequences. Japanese Journal of Tropical Medicine & Hygiene, 29, 261‾266.
  • Pramual, P. & Wongpakam, K. (2013) Population genetics of the high elevation black fly Simulium (Nevermannia) feuerborni Edwards in Thailand. Journal of Entomological Science, 16, 298-308.
  • Pramual, P., Thaijarern, J., Azirun, M., Ya'cob, Z., Hadi, U. & Takaoka, H. (2015) Cytogenetic and molecular evidence of additional cryptic diversity in high elevation black fly Simulium feuerborni (Diptera: Simuliidae) populations in Southeast Asia. Journal of Medical Entomology, 52, 829-836.
  • Procunier, W.S. (1989) Cytological approaches to simuliid biosystematics in relation to the epidemiology and control of human onchocerciasis. Genome, 32, 559‾569.
  • Rothfels, K.H. (1979) Cytotaxonomy of black flies (Simuliidae). Annual Review of Entomology, 24, 507-539.
  • Rothfels, K., Feraday, R. & Kaneps, A. (1978) A cytological description of sibling species of Simulium venustum and S. verecundum with standard maps for the subgenus Simulium Davies [sic] (Diptera). Canadian Journal of Zoology, 56, 1110-1128.
  • Takaoka, H., Thapa, S. & Henry, W. (2010) Description of two new species of Simulium (Gomphostilbia) (Diptera: Simuliidae) from Darjeeling, India. Medical Entomology & Zoology, 61, 105‾110.
  • Takaoka, H., Thapa, S. & Henry, W. (2011) A new species of black fly (Diptera: Simuliidae) with an entirely yellow thorax from Darjeeling, India. Zootaxa, 2824, 62-68.
  • Tangkawanit, U., Kuvangkadilok, C., Baimai, V. & Adler, P.H. (2009) Cytosystematics of the Simulium tuberosum group (Diptera: Simuliidae) in Thailand. Zoological Journal of the Linnean Society, 155, 289‾315.
  • Thanwasi, A., Kuvangkadilok, C. & Baimai, V. (2006) Molecular phylogeny of black flies (Diptera: Simuliidae) from Thailand, using ITS rDNA. Genetica, 128, 177‾204.
  • Thapa, S., Procunier, W., Henry, W. & Chhetri, S. (2014) Heterochromatin and sibling species of Simulium praelargum s.l. (Diptera: Simuliidae). Genome, 57, 223-232.