( Figs 1, 2, 7, 8; Table 4)
Material examined. Calypso hydrothermal vent field ( Southern vent field), southwest of White Island, Bay of Plenty: Holotype — NIWA 32136, University of Kiel Stn SO192-2/3, 37.688° S, 177.123° W, 192 m, collected by TV Grab, 27 April 2007. Paratypes — NIWA 32135, 32140, University of Kiel Stn SO192-2/7, 37.688° S, 177.123° W, 191 m, collected by ROV, 28 April 2007; NIWA 52839, IFM GEOMAR Stn SO135/110, 37.688° S, 177.122° E, 189–192 m, collected by rock dredge, 11 Oct 1998.
Other material. NIWA 52838, IFM GEOMAR Stn SO135/103, 37.695° S, 177.101° E, 179–181 m, collected by rock dredge, 10 Oct 1998; NIWA 52851, IFM GEOMAR Stn SO135/110, 37.688° S, 177.122° E, 189–192 m, collected by rock dredge, 11 Oct 1998.
Type location & distribution. Calypso hydrothermal vent field, Bay of Plenty, New Zealand, 179–192 m.
Description. Thickly encrusting on hard rocky substrate, appearing ‘stretched’ in places, about 5–10 mm thick, growing in patches of about 50–100 mm wide. Encrustations may give rise to low mounds, lobes, flabby expanses and finger-shaped projection, 5–10 mm diameter ( Fig. 7A, B). Oscules are not obvious in life but appear to be at the ends of some fingers in in situ images. The surface is macroscopically smooth to undulating in life, with a stretched, slightly micro-knobbed appearance when out of water ( Fig. 7C). Sediment catches in places where ectosomal spicules protrude beyond the surface rendering the texture slightly fuzzy ( Fig. 7D). Texture in life slightly compressible, rubbery, corky, flexible. Colour in preservative ranges from light to dark cream yellow, to peach-coloured, to deep dull pink (NIWA 52838).
Skeleton. Choanosome composed of wavy tracts of large tylostyles aligned longitudinally through the axes of flabellate and finger-like portions of specimens ( Fig. 7E), diverging towards the surface in encrusting portions ( Fig. 7F, G). Tracts range from about 100–1100 µm wide and are interspersed with numerous free tylostyles. About 1000 to 1500 µm below the surface, tracts branch and diverge towards the surface, terminating below well separated surface bouquets of smaller tylostyles, the sharp tips of which project cleanly from the surface by about 60–150 µm.
Spicules. Megascleres ( Table 4; Fig. 8) are tylostyles with well-developed, spherical heads with a slight protrusion at the tip, ranging in length from about 150–920 µm long and 7–25 µm thick, slightly sinuous and unevenly thickened along the straight shaft, thicker in the upper half. Examination of histological sections of the ectosome and choanosome reveal at least two clear size categories of tylostyles, the smaller in the ectosomal brushes (170–350 µm long), the largest in the deep choanosomal tracts (500–920 µm long).
Specimen | Ectosomal tylostyles | Choanosomal tylostyles |
Holotype NIWA 32136 | 223 (180–290) × 8 (6–10) | 505 (430–800) × 17 (17–19) |
Paratype NIWA 32135 | 240 (170–350) × 8 (8–10) | 609 (450–830) × 13 (12–19) |
Paratype NIWA 52851 | 222 (150–300) × 8 (7–11) | 805 (630–870) × 16 (10–23) |
Paratype NIWA 52839 | 284 (180–400) × 7 (6–8) | 737 (450–850) × 14 (13–19) |
NIWA 52838 | 266 (200–380) × 7 (6–8) | 719 (460–920) × 20 (15–25) |
Substrate, depth range and ecology. Attached to volcaniclastic rock covered in sediment and associated with high densities of other sponges and an undescribed orange anemone, 179– 192 m.
Etymology. Named for the first record of the genus in the New Zealand region.
Remarks. In the Pacific Ocean region, two species were described from the Southern California Bight [ P. mexicensis (de Laubenfels, 1935), P. sisyrnus (de Laubenfels, 1930)], Australia [ P. epiphytum ( Lamarck, 1815); P. proteus ( Hentschel, 1909), and Chile [ P. epiphytoides ( Thiele, 1905)]. The most common species in this region, stretching from the Indian Ocean to Palau in the Central Pacific, is P. diversicolor Becking & Lim (as Suberites), often found in land-locked marine lakes ( Kelly & Bell 2015).
In terms of spicule dimensions, the closest species to P. novaezelandiae sp. nov. are P. mexicensis from the South Californian Bight, with tylostyles up to 1000 µm long ( Table 5), and P. proteus from Shark Bay, southwestern Australia, with tylostyles ranging from 15 8–808 µm long ( Table 5). The Californian species has much larger megascleres than P. novaezelandiae sp. nov., and according to de Laubenfels (1935), the sponge is a thin encrustation on a large spicule of hexactinellid origin. The choanosome has a confused arrangement with bouquets at the surface, but there is no appreciable difference between the lengths of the tylostyles in the ectosome and choanosome. Protosuberites proteus from tropical Shark Bay in Western Australia, has very similar spiculation to P. novaezelandiae sp. nov., but is a shallow water sponge collected from estuaries and rocky reefs between 3 and 12 m deep. The sponge is much thicker (up to 6 cm thick) than the New Zealand species ( 5–10 mm thick) which is found in much deep waters ( 179–192 m). The two species are separated primarily on their disjunct geographic distributions (shallow tropical estuarine Western Australia vs deep-water hydrothermal vent field in temperate eastern New Zealand).
Most authors do not differentiate ectosomal and choanosomal categories of megascleres in species of Protosuberites from the South Pacific and Southern Ocean region ( Table 5). However, in those species with
Locality & depth | Morphology & colour | Skeleton | Spicule dimensions | Comment |
Protosuberites mexicensis (de Laubenfels, 1935) | ||||
South Californian Bight | Encrustation on a | Choanosome confused | tylostyles | De Laubenfels (1935: 9) considered the architecture |
hexactinellid spicule, 2 cm | with bouquets of | 1000 × 20 µm | of the sponge to be to be clearly that of | |
long, forming a wedge- | spicules at the surface, | Laxosuberites (now Protosuberites) and that the | ||
shaped process extending 1 | the spicules being the | enormous size of the tylostyles was the most | ||
cm from substrate; drab, | same size as those in the | characteristic feature of the species. | ||
soft, surface faintly hispid | choanosome | |||
Protosuberites sisyrnus (de Laubenfels, 1930) | ||||
San Pedro, South Californian | Encrusting, 1–2 mm thick, | Choanosome very | tylostyles | Note that de Laubenfels (1935: 9) misquoted the |
Bight, 45 m | surrounding worm-tubes | scanty, when present of | 27 5-480 µm long | size of the tylostyles in this species as 27–480 um |
and algae, colour drab, | a confused nature; | 8 × 20 µm thick | long, not 275–480 um long, which is correct. | |
surface velvety | ectosome with erect | |||
tylostyles | ||||
Protosuberites epiphytum (Lamarck, 1815) | ||||
South Australia | Encrusts the thallus of a | Possesses ectosomal | 180 (90–270) × 4 (3–6) µm (Van | Van Soest and De Kluijver (2003) differentiated |
fucoid? brown alga, | brushes of tylostyles in | Soest and De Kluijver 2003) | common northeast Atlantic sponges included under | |
possibly in symbiotic | addition to single or | Prosuberites epiphytum, from the South Australian | ||
association (Van Soest and | bundles of choanosomal | type and other regional specimens, and transferred | ||
De Kluijver 2003) | tylostyles | the Atlantic specimens to a new species, | ||
(Van Soest and De | Protosuberites denhartogi Van Soest and De | |||
Kluijver 2003) | Kluijver, 2003. Prosuberites epiphytum (and thus, | |||
the new species as well) were transferred from | ||||
Prosuberites to Protosuberites. |
……continued on the next page
Locality & depth | Morphology & colour | Skeleton | Spicule dimensions | Comment |
Protosuberites proteus (Hentschel, 1909) | ||||
Shark Bay, Western Australia; | Solid or free sponges of | Skeleton consists of a | tylostyles | Two varieties were originally described for |
Rocky bottom with coral, muddy | massive, spherical, ovate to | dense network of more | 158–808 µm long | Laxosuberites proteus; var. donatioides (Hentschel, |
seabed with algae, 3–12.5 m | pillow-shaped form, 11 cm | or less distinct tracts of | 5 × 11 µm thick | 1909) and var. tectulum (Hentschel, 1909). Hooper |
long, 8 cm wide, 6 cm high. | spicules, between which | & Wiedenmayer (1994) assigned both varieties to | ||
Surface warty, beaded, or | lie loose spicules in a | the nominal species proteus. | ||
ridged, or even wrinkled or | confused arrangement. | |||
smooth. The color in | Ectosome composed of | |||
alcohol is brownish gray | brushes arising from | |||
choanosomal tracts | ||||
Protosuberites epiphytoides (Thiele, 1905) | ||||
Juan Ferdandez, Chile | Small encrustations c. half | Skeleton composed of | tylostyles to subtylostyles | Thiele (1905) considered this species to be closer to |
to 1 cm square, 0.5 mm | abundant tylostyles that | 400–450 µm, barely 500 µm long | Topsent’s Prosuberites, even though the tylostyles | |
thick. Smooth, firm, light | lie oblique to the | 8 µm thick | were not strictly arising from the base. This species | |
gray, no orifices visible. | surface, with none | is perhaps, more easily recognisable as | ||
projecting from the | Protosuberites where it presently sits. | |||
surface appreciably. | ||||
Protosuberites hendricksi Samaai & Gibbons, 2005 | ||||
West Coast, South Africa, 2–5 m | Thin encrusting sponge | Choanosomal skeleton a | tylostyles | The ectosome is formed from the radiating brushes |
bearing erect processes, | confused, irregular | 564 (510–601) × 12 µm | at the ends of choanosomal tracts, but these are not | |
texture tough, bright yellow | reticulation of bundles | 415 (345–473) × 9 µm | obvious in Samaai & Gibbons (2005, Fig. 19). The | |
in life | of tylostyles. Spicules | 231 (182–255) × 5 µm | skeleton is quite lax but appears to conform to | |
radiate at the surface | Protosuberites. The authors recognise three size | |||
categories, however, the larger two are quite similar | ||||
and could be considered to be one and the same | ||||
choanosomal category, as in other species. |
choanosomal tracts that diverge below the ectosome, such as in P. hendricksi ( Table 5) and in P. novaezelandiae sp. nov., there is a striking difference in the lengths of spicules in these species (e.g. Fig. 5F; Table 4), and some evidence that, as in many Suberitidae genera, a subectosomal category may also be discernible, but difficult to measure (see Samaai & Gibbons 2005).
Protosuberites is well represented in the Atlantic Ocean and further north (13 species): North and Celtic Seas [ P. incrustans (Hansen) and sensu (Stephens); P. denhartogi Van Soest & de Kluijver; P. durus (Stephens)]; Western Mediterranean and Black Seas [ P. brevispinus (de Laubenfels); P. ectyoninus (Topsent); P. mereui Manconi; P. modestus (Pulitzer-Finali); P. prototipus; P. rugosus (Topsent)]; North Atlantic [ P. capillitium (Topsent); P. ferrerhernandezi (Boury-Enault & Lopes)]; Western Tropical Atlantic [ P. geracei (Van Soest & Sass)], and across the Western Indian Ocean [ P. reptans (Kirkpatrick) and P. hendricksi Samaai & Gibbons from South Africa; P. longispiculus (Burton) from the Maldives] and Southeast Asia, where the earliest species were described from the brackish, land-locked Chilka Lake of eastern India [ P. aquaedulcioris (Annandale); P. lacustris (Annandale)] and P. collaris Annandale from Krakatau, Indonesia. A sixth species has been described from Cheju Island, Korea, P. nestus (Sim & Kim). A review of all species and their skeletal architecture and spicule dimensions would be very useful but is beyond the scope of this work.