Biotin: MedlinePlus Supplements

T What is it?
P Biotin is a vitamin that is found in small amounts in numerous foods.
P Biotin is used for preventing and treating biotin deficiency associated with pregnancy, long-term tube feeding, malnutrition, and rapid weight loss. It is also used orally for hair loss, brittle nails, skin rash in infants (seborrheic dermatitis), diabetes, and mild depression.

T How effective is it?
P Natural Medicines Comprehensive Database rates effectiveness based on scientific evidence according to the following scale: Effective, Likely Effective, Possibly Effective, Possibly Ineffective, Likely Ineffective, Ineffective, and Insufficient Evidence to Rate.
P The effectiveness ratings for BIOTIN are as follows:
t Likely effective for...
- Treating and preventing biotin deficiency. Symptoms of deficiency include thinning of the hair (often with loss of hair color), and red scaly rash around the eyes, nose, and mouth. Other symptoms include depression, listlessness, hallucinations, and tingling in the arms and legs. There is some evidence that cigarette smoking may cause mild biotin deficiency.
t Possibly ineffective for...
- Skin rash in infants (seborrheic dermatitis).
t Insufficient evidence to rate effectiveness for...
- Hair loss. There is some preliminary evidence that hair loss can be reduced when biotin is taken by mouth in combination with zinc while a cream containing the chemical compound clobetasol propionate (Olux, Temovate) is applied to the skin.
- Diabetes. Biotin alone doesn’t seem to affect blood sugar levels in people with type 2 diabetes. However, there is some evidence that a combination of biotin and chromium (Diachrome, Nutrition 21) might lower blood sugar in people with diabetes, whose diabetes is poorly controlled by prescription medicines. Other early evidence shows that the same combination reduces ratios of total cholesterol levels to “good” high-density lipoprotein (HDL) cholesterol, “bad” low-density lipoprotein (LDL) cholesterol to HDL cholesterol, and non-HDL to HDL cholesterol in people with type 2 diabetes.
- Diabetic nerve pain. There is some evidence that biotin can reduce nerve pain in people with diabetes.
- Brittle fingernails and toenails. Biotin might increase the thickness of fingernails and toenails in people with brittle nails.
- Other conditions.
P More evidence is needed to rate biotin for these uses.

T How does it work?
P Biotin is an important component of enzymes in the body that break down certain substances like fats, carbohydrates, and others.
P There isn’t a good laboratory test for detecting biotin deficiency, so this condition is usually identified by its symptoms, which include thinning of the hair (frequently with loss of hair color) and red scaly rash around the eyes, nose, and mouth. Nervous system symptoms include depression, exhaustion, hallucinations, and tingling of the arms and legs. There is some evidence that diabetes could result in biotin deficiency.

T Are there safety concerns?
P Biotin is LIKELY SAFE for most people when taken appropriately and by mouth. Biotin is well tolerated when used at recommended dosages. It is POSSIBLY SAFE when injected into the muscles and used appropriately.
t Special precautions & warnings:
P Pregnancy and breast-feeding: Biotin is POSSIBLY SAFE when used in recommended amounts during pregnancy and breast-feeding.
P Kidney dialysis: People receiving kidney dialysis may need extra biotin. Check with your health care provider.

T Are there interactions with medications?
P Minor
P Be watchful with this combination.
P Medications changed by the liver (Cytochrome P450 1B1 (CYP1B1) substrates)
P Some medications are changed and broken down by the liver. Biotin might decrease how quickly the liver breaks down some medications. Taking biotin along with some medications that are changed by the liver might increase the effects and side effects of some medications. Before taking biotin, talk to your healthcare provider if you take any medications that are changed by the liver.
P Some of these medications that are changed by the liver include clozapine (Clozaril), cyclobenzaprine (Flexeril), fluvoxamine (Luvox), haloperidol (Haldol), imipramine (Tofranil), mexiletine (Mexitil), olanzapine (Zyprexa), pentazocine (Talwin), propranolol (Inderal), tacrine (Cognex), theophylline, zileuton (Zyflo), zolmitriptan (Zomig), and others.

T Are there interactions with herbs and supplements?
P Alpha-lipoic acid
P Alpha-lipoic acid and biotin taken together can each reduce the body's absorption of the other.
P Vitamin B5 (pantothenic acid)
P Biotin and vitamin B5 taken together can each reduce the body's absorption of the other.

T Are there interactions with foods?
P Egg whites
P Raw egg white contains a substance that binds biotin in the intestine and keeps it from being absorbed. Eating 2 or more uncooked egg whites daily for several months has caused biotin deficiency that is serious enough to produce symptoms.

T What dose is used?
P The appropriate dose of biotin depends on several factors such as the user’s age, health, and several other conditions. At this time there is not enough scientific information to determine an appropriate range of doses for biotin. Keep in mind that natural products are not always necessarily safe and dosages can be important. Be sure to follow relevant directions on product labels and consult your pharmacist or physician or other healthcare professional before using.
P There is no recommended dietary allowance (RDA) established for biotin. The adequate intakes (AI) for biotin are 7 mcg for infants 0-12 months, 8 mcg for children 1-3 years, 12 mcg for children 4-8 years, 20 mcg for children 9-13 years, 25 mcg for adolescents 14-18 years, 30 mcg for adults over 18 years and pregnant women, and 35 mcg for breast-feeding women.

T Other names
P Biotina, Biotine, Biotine-D, Coenzyme R, D-Biotin, Vitamin B7, Vitamin H, Vitamine B7, Vitamine H, W Factor, Cis-hexahydro-2-oxo-1H-thieno[3,4-d]-imidazole-4-valeric Acid.

T Methodology
P To learn more about how this article was written, please see the Natural Medicines Comprehensive Database methodology.

T References
- du, Vigneaud, V, Melville, D. B., Folkers, K., Wolf, D. E., Mozingo, D. E., Keresztesy, J. C., and Harris, S. A. The structure of biotin: a study of desthiobiotin. J.Biol.Chem 1942;:475-485.
- Kogl, F. and Tonnis, B. Ober das Bios-Problem. Darstellung von krystallisiertem Biotin aus Eigelb. Z.Physiol.Chem 1932;:43-73.
- Sydenstricker, V. P., Singal, S. A., Briggs, A. P., Devaughn, N. M., and Isbell, H. Observations on the "egg white injury" in man and its cure with biotin concentrate. J Am Med Assn 1942;:199-200.
- Eakin, R. E., Snell, E. E., and Williams, R. J. Concentrationand assay of avidin, injury-producing agents in raw egg white. J Biol Chem. 1941;:535-43.
- Tarmina, M. S. Self-selected diet of adult women with families. 1992;257.
- Ozand, P. T., Gascon, G. G., Al Essa, M., Joshi, S., Al Jishi, E., Bakheet, S., Al Watban, J., Al Kawi, M. Z., and Dabbagh, O. Biotin-responsive basal ganglia disease: a novel entity. Brain 1998;121 ( Pt 7):1267-1279. View abstract.
- Wallace, J. C., Jitrapakdee, S., and Chapman-Smith, A. Pyruvate carboxylase. Int J Biochem.Cell Biol. 1998;30:1-5. View abstract.
- Zempleni, J., Green, G. M., Spannagel, A. W., and Mock, D. M. Biliary excretion of biotin and biotin metabolites is quantitatively minor in rats and pigs. J Nutr. 1997;127:1496-1500. View abstract.
- Burnett, R., Guichard, Y., and Barale, E. Immunohistochemistry for light microscopy in safety evaluation of therapeutic agents: an overview. Toxicology 4-11-1997;119:83-93. View abstract.
- Zempleni, J., McCormick, D. B., and Mock, D. M. Identification of biotin sulfone, bisnorbiotin methyl ketone, and tetranorbiotin-l-sulfoxide in human urine. Am.J Clin.Nutr. 1997;65:508-511. View abstract.
- van der Knaap, M. S., Jakobs, C., and Valk, J. Magnetic resonance imaging in lactic acidosis. J Inherit.Metab Dis. 1996;19:535-547. View abstract.
- Shriver, B. J., Roman-Shriver, C., and Allred, J. B. Depletion and repletion of biotinyl enzymes in liver of biotin-deficient rats: evidence of a biotin storage system. J Nutr. 1993;123:1140-1149. View abstract.
- Biotin and fatty acid requirements for lymphocyte response. Nutr.Rev. 1980;38:126-127. View abstract.
- McMurray, D. N. Cell-mediated immunity in nutritional deficiency. Prog.Food Nutr.Sci 1984;8(3-4):193-228. View abstract.
- Ammann, A. J. New insight into the causes of immunodeficiency disorders. J Am.Acad.Dermatol. 1984;11(4 Pt 1):653-660. View abstract.
- Petrelli, F., Moretti, P., and Paparelli, M. Intracellular distribution of biotin-14COOH in rat liver. Mol.Biol.Rep. 2-15-1979;4:247-252. View abstract.
- Zlotkin, S. H., Stallings, V. A., and Pencharz, P. B. Total parenteral nutrition in children. Pediatr.Clin.North Am. 1985;32:381-400. View abstract.
- Dreizen, S. Nutrition and the immune response -- a review. Int J Vitam.Nutr.Res. 1979;49:220-228. View abstract.
- Bowman, B. B., Selhub, J., and Rosenberg, I. H. Intestinal absorption of biotin in the rat. J Nutr. 1986;116:1266-1271. View abstract.
- Magnuson, N. S. and Perryman, L. E. Metabolic defects in severe combined immunodeficiency in man and animals. Comp Biochem.Physiol B 1986;83:701-710. View abstract.
- Nyhan, W. L. Inborn errors of biotin metabolism. Arch.Dermatol. 1987;123:1696-1698a. View abstract.
- Sweetman, L. and Nyhan, W. L. Inheritable biotin-treatable disorders and associated phenomena. Annu.Rev.Nutr. 1986;6:317-343. View abstract.
- Brenner, S. and Horwitz, C. Possible nutrient mediators in psoriasis and seborrheic dermatitis. II. Nutrient mediators: essential fatty acids; vitamins A, E and D; vitamins B1, B2, B6, niacin and biotin; vitamin C selenium; zinc; iron. World Rev.Nutr.Diet. 1988;55:165-182. View abstract.
- Said, H. M., Mock, D. M., and Collins, J. C. Regulation of intestinal biotin transport in the rat: effect of biotin deficiency and supplementation. Am.J Physiol 1989;256(2 Pt 1):G306-G311. View abstract.
- Miller, S. J. Nutritional deficiency and the skin. J Am.Acad.Dermatol. 1989;21:1-30. View abstract.
- Michalski, A. J., Berry, G. T., and Segal, S. Holocarboxylase synthetase deficiency: 9-year follow-up of a patient on chronic biotin therapy and a review of the literature. J Inherit.Metab Dis. 1989;12:312-316. View abstract.
- Colombo, V. E., Gerber, F., Bronhofer, M., and Floersheim, G. L. Treatment of brittle fingernails and onychoschizia with biotin: scanning electron microscopy. J Am.Acad.Dermatol. 1990;23(6 Pt 1):1127-1132. View abstract.
- Pletsityi, K. D. [Vitamins and immunity. Biotin, pantothenic acid and riboflavin]. Vopr.Pitan. 1990;:18-22. View abstract.
- Petronzelli, F., Pelliccia, A., Anastasi, A. M., Lindstedt, R., Manganello, S., Ferrari, L. E., Albertoni, C., Leoni, B., Rosi, A., D'Alessio, V., Deiana, K., Paganelli, G., and De, Santis R. Therapeutic use of avidin is not hampered by antiavidin antibodies in humans. Cancer Biother.Radiopharm. 2010;25:563-570. View abstract.
- Daniells, S. and Hardy, G. Hair loss in long-term or home parenteral nutrition: are micronutrient deficiencies to blame? Curr.Opin.Clin.Nutr.Metab Care 2010;13:690-697. View abstract.
- Becker, L. C., Bergfeld, W. F., Belsito, D. V., Klaassen, C. D., Marks, J. G., Jr., Shank, R. C., Slaga, T. J., Snyder, P. W., and Alan, Andersen F. Final report of the safety assessment of allantoin and its related complexes. Int J Toxicol. 2010;29(3 Suppl):84S-97S. View abstract.
- Mall, G. K., Chew, Y. C., and Zempleni, J. Biotin requirements are lower in human Jurkat lymphoid cells but homeostatic mechanisms are similar to those of HepG2 liver cells. J Nutr. 2010;140:1086-1092. View abstract.
- Lesch, H. P., Kaikkonen, M. U., Pikkarainen, J. T., and Yla-Herttuala, S. Avidin-biotin technology in targeted therapy. Expert.Opin.Drug Deliv. 2010;7:551-564. View abstract.
- Wolf, B. Clinical issues and frequent questions about biotinidase deficiency. Mol.Genet.Metab 2010;100:6-13. View abstract.
- Rogovik, A. L., Vohra, S., and Goldman, R. D. Safety considerations and potential interactions of vitamins: should vitamins be considered drugs? Ann.Pharmacother. 2010;44:311-324. View abstract.
- O'Keefe, S. J., Ou, J., Aufreiter, S., O'Connor, D., Sharma, S., Sepulveda, J., Fukuwatari, T., Shibata, K., and Mawhinney, T. Products of the colonic microbiota mediate the effects of diet on colon cancer risk. J Nutr. 2009;139:2044-2048. View abstract.
- Zempleni, J., Hassan, Y. I., and Wijeratne, S. S. Biotin and biotinidase deficiency. Expert.Rev.Endocrinol.Metab 11-1-2008;3:715-724. View abstract.
- Tsao, C. Y. Current trends in the treatment of infantile spasms. Neuropsychiatr.Dis.Treat. 2009;5:289-299. View abstract.
- Grammatikopoulou, M. G., Daskalou, E., and Tsigga, M. Diet, feeding practices, and anthropometry of children and adolescents with cerebral palsy and their siblings. Nutrition 2009;25:620-626. View abstract.
- Rodriguez-Melendez, R. and Zempleni, J. Nitric oxide signaling depends on biotin in Jurkat human lymphoma cells. J Nutr. 2009;139:429-433. View abstract.
- Vlaming, M. L., Lagas, J. S., and Schinkel, A. H. Physiological and pharmacological roles of ABCG2 (BCRP): recent findings in Abcg2 knockout mice. Adv.Drug Deliv.Rev. 1-31-2009;61:14-25. View abstract.
- Sedel, F., Lyon-Caen, O., and Saudubray, J. M. [Treatable hereditary neuro-metabolic diseases]. Rev.Neurol.(Paris) 2007;163:884-896. View abstract.
- Gralla, M., Camporeale, G., and Zempleni, J. Holocarboxylase synthetase regulates expression of biotin transporters by chromatin remodeling events at the SMVT locus. J Nutr.Biochem. 2008;19:400-408. View abstract.
- Sydenstricker, V. P., Singal, S. A., Briggs, A. P., Devaughn, N. M., and Isbell, H. PRELIMINARY OBSERVATIONS ON "EGG WHITE INJURY" IN MAN AND ITS CURE WITH A BIOTIN CONCENTRATE. Science 2-13-1942;95:176-177. View abstract.
- Scheinfeld, N., Dahdah, M. J., and Scher, R. Vitamins and minerals: their role in nail health and disease. J Drugs Dermatol. 2007;6:782-787. View abstract.
- du, Vigneaud, V. THE STRUCTURE OF BIOTIN. Science 11-20-1942;96:455-461. View abstract.
- Spector, R. and Johanson, C. E. Vitamin transport and homeostasis in mammalian brain: focus on Vitamins B and E. J Neurochem. 2007;103:425-438. View abstract.
- Mock, D. M. Skin manifestations of biotin deficiency. Semin.Dermatol. 1991;10:296-302. View abstract.
- Dashwood, R. H. and Ho, E. Dietary histone deacetylase inhibitors: from cells to mice to man. Semin.Cancer Biol. 2007;17:363-369. View abstract.
- Bolander, F. F. Vitamins: not just for enzymes. Curr.Opin.Investig.Drugs 2006;7:912-915. View abstract.
- Hassan, Y. I. and Zempleni, J. Epigenetic regulation of chromatin structure and gene function by biotin. J Nutr. 2006;136:1763-1765. View abstract.
- Boas, M. A. The Effect of Desiccation upon the Nutritive Properties of Egg-white. Biochem.J 1927;21:712-724. View abstract.
- Said, H. M. and Mohammed, Z. M. Intestinal absorption of water-soluble vitamins: an update. Curr.Opin.Gastroenterol. 2006;22:140-146. View abstract.
- Vilches-Flores, A. and Fernandez-Mejia, C. [Effect of biotin upon gene expression and metabolism]. Rev.Invest Clin. 2005;57:716-724. View abstract.
- Prasad, A. N. and Seshia, S. S. Status epilepticus in pediatric practice: neonate to adolescent. Adv.Neurol. 2006;97:229-243. View abstract.
- Suzuki, Y., Yang, X., Aoki, Y., Kure, S., and Matsubara, Y. Mutations in the holocarboxylase synthetase gene HLCS. Hum.Mutat. 2005;26:285-290. View abstract.
- Wilson, C. J., Myer, M., Darlow, B. A., Stanley, T., Thomson, G., Baumgartner, E. R., Kirby, D. M., and Thorburn, D. R. Severe holocarboxylase synthetase deficiency with incomplete biotin responsiveness resulting in antenatal insult in samoan neonates. J Pediatr. 2005;147:115-118. View abstract.
- Zempleni, J. Uptake, localization, and noncarboxylase roles of biotin *. Annu.Rev Nutr. 2005;25:175-196. View abstract.
- Kothapalli, N., Camporeale, G., Kueh, A., Chew, Y. C., Oommen, A. M., Griffin, J. B., and Zempleni, J. Biological functions of biotinylated histones. J Nutr.Biochem. 2005;16:446-448. View abstract.
- Mock, D. M. Marginal biotin deficiency is teratogenic in mice and perhaps humans: a review of biotin deficiency during human pregnancy and effects of biotin deficiency on gene expression and enzyme activities in mouse dam and fetus. J Nutr.Biochem. 2005;16:435-437. View abstract.
- Leon-Del-Rio, A. Biotin-dependent regulation of gene expression in human cells. J Nutr.Biochem. 2005;16:432-434. View abstract.
- Fernandez-Mejia, C. Pharmacological effects of biotin. J Nutr.Biochem. 2005;16:424-427. View abstract.
- Dakshinamurti, K. Biotin--a regulator of gene expression. J Nutr.Biochem. 2005;16:419-423. View abstract.
- Rodriguez-Melendez, R., Griffin, J. B., Sarath, G., and Zempleni, J. High-Throughput Immunoblotting Identifies Biotin-Dependent Signaling Proteins in HepG2 Hepatocarcinoma Cells. J Nutr. 2005;135:1659-1666. View abstract.
- Tong, L. Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery. Cell Mol.Life Sci 2005;62:1784-1803. View abstract.
- Zeng, W. Q., Al Yamani, E., Acierno, J. S., Jr., Slaugenhaupt, S., Gillis, T., MacDonald, M. E., Ozand, P. T., and Gusella, J. F. Biotin-responsive basal ganglia disease maps to 2q36.3 and is due to mutations in SLC19A3. Am.J Hum.Genet. 2005;77:16-26. View abstract.
- Baumgartner, M. R. Molecular mechanism of dominant expression in 3-methylcrotonyl-CoA carboxylase deficiency. J Inherit.Metab Dis. 2005;28:301-309. View abstract.
- Carey, P. R., Sonnichsen, F. D., and Yee, V. C. Transcarboxylase: one of nature's early nanomachines. IUBMB.Life 2004;56:575-583. View abstract.
- Pacheco-Alvarez, D., Solorzano-Vargas, R. S., Gravel, R. A., Cervantes-Roldan, R., Velazquez, A., and Leon-Del-Rio, A. Paradoxical regulation of biotin utilization in brain and liver and implications for inherited multiple carboxylase deficiency. J Biol Chem. 12-10-2004;279:52312-52318. View abstract.
- Crisp, S. E., Griffin, J. B., White, B. R., Toombs, C. F., Camporeale, G., Said, H. M., and Zempleni, J. Biotin supply affects rates of cell proliferation, biotinylation of carboxylases and histones, and expression of the gene encoding the sodium-dependent multivitamin transporter in JAr choriocarcinoma cells. Eur.J Nutr. 2004;43:23-31. View abstract.
- Said, H. M. Recent advances in carrier-mediated intestinal absorption of water-soluble vitamins. Annu.Rev.Physiol 2004;66:419-446. View abstract.
- Rodriguez-Melendez, R. and Zempleni, J. Regulation of gene expression by biotin (review). J Nutr.Biochem. 2003;14:680-690. View abstract.
- Snodgrass, S. R. Vitamin neurotoxicity. Mol.Neurobiol. 1992;6:41-73. View abstract.
- POTH, E. J. Intestinal antisepsis in surgery. J Am.Med Assoc. 12-26-1953;153:1516-1521. View abstract.
- Barry, M. A., Campos, S. K., Ghosh, D., Adams, K. E., Mok, H., Mercier, G. T., and Parrott, M. B. Biotinylated gene therapy vectors. Expert.Opin.Biol.Ther. 2003;3:925-940. View abstract.
- Bohrer, D., do Nascimento, P. C., Binotto, R., Becker, E., and Pomblum, S. Contribution of the raw material to the aluminum contamination in parenterals. JPEN J Parenter.Enteral Nutr. 2002;26:382-388. View abstract.
- Esposito, S. Immune system and surgical site infection. J Chemother. 2001;13 Spec No 1:12-16. View abstract.
- Dror, Y., Stern, F., Berner, Y. N., Kaufmann, N. A., Berry, E., Maaravi, Y., Altman, H., Cohen, A., Leventhal, A., and Kaluski, D. N. [Micronutrient (vitamins and minerals) supplementation for the elderly, suggested by a special committee nominated by Ministry of Health]. Harefuah 2001;140:1062-7, 1117. View abstract.
- Jaffrey, S. R. and Snyder, S. H. The biotin switch method for the detection of S-nitrosylated proteins. Sci STKE. 6-12-2001;2001:l1. View abstract.
- Campistol, J. [Convulsions and epileptic syndromes of the newborn infant. Forms of presentation, study and treatment protocols]. Rev.Neurol. 10-1-2000;31:624-631. View abstract.
- Prasad, P. D. and Ganapathy, V. Structure and function of mammalian sodium-dependent multivitamin transporter. Curr.Opin.Clin.Nutr.Metab Care 2000;3:263-266. View abstract.
- Livaniou, E., Costopoulou, D., Vassiliadou, I., Leondiadis, L., Nyalala, J. O., Ithakissios, D. S., and Evangelatos, G. P. Analytical techniques for determining biotin. J Chromatogr.A 6-9-2000;881(1-2):331-343. View abstract.
- de Wolf, F. A. and Brett, G. M. Ligand-binding proteins: their potential for application in systems for controlled delivery and uptake of ligands. Pharmacol.Rev. 2000;52:207-236. View abstract.
- Bliss, D. Z., McLaughlin, J., Jung, H. J., Lowry, A., Savik, K., and Jensen, L. Comparison of the nutritional composition of diets of persons with fecal incontinence and that of age- and gender-matched controls. J Wound.Ostomy.Continence.Nurs. 2000;27:90-97. View abstract.
- Zempleni, J. and Mock, D. M. Utilization of biotin in proliferating human lymphocytes. J Nutr. 2000;130(2S Suppl):335S-337S. View abstract.
- Yoshikawa, K. [Vitamin and dermatology]. Nippon Rinsho 1999;57:2385-2389. View abstract.
- Narisawa, K. [Molecular basis of vitamin-responsive inborn errors of metabolism]. Nippon Rinsho 1999;57:2301-2306. View abstract.
- Furukawa, Y. [Enhancement of glucose-induced insulin secretion and modification of glucose metabolism by biotin]. Nippon Rinsho 1999;57:2261-2269. View abstract.
- Zempleni, J. and Mock, D. M. Advanced analysis of biotin metabolites in body fluids allows a more accurate measurement of biotin bioavailability and metabolism in humans. J Nutr. 1999;129(2S Suppl):494S-497S. View abstract.
- Hymes, J. and Wolf, B. Human biotinidase isn't just for recycling biotin. J Nutr. 1999;129(2S Suppl):485S-489S. View abstract.
- Zempleni J, Mock DM. Biotin biochemistry and human requirements. J Nutr Biochem. 1999 Mar;10:128-38. View abstract.
- Eakin RE, Snell EE, and Williams RJ. Concentrationand assay of avidin, injury-producing agents in raw egg white. J Biol Chem. 1941;:535-43.
- Spencer RP and Brody KR. Biotin transport by small intestine of rat, hamster, and other species. Am J Physiol. 1964 Mar;206:653-7. View abstract.
- Zempleni J, Wijeratne SS, Hassan YI. Biotin. Biofactors. 2009 Jan-Feb;35:36-46. View abstract.
- Green NM. Avidin. 1. The use of (14-C)biotin for kinetic studies and for assay. Biochem. J. 1963;89:585-591. View abstract.
- Schulpis KH, Georgala S, Papakonstantinou ED, et al. The effect of isotretinoin on biotinidase activity. Skin Pharmacol Appl Skin Physiol. 1999 Jan-Apr;12:28-33. View abstract.
- Rodriguez-Melendez R, Griffin JB, Zempleni J. Biotin supplementation increases expression of the cytochrome P450 1B1 gene in Jurkat cells, increasing the occurrence of single-stranded DNA breaks. J Nutr. 2004 Sep;134:2222-8. View abstract.
- Grundy WE, Freed M, Johnson H.C., et al. The effect of phthalylsulfathiazole (sulfathalidine) on the excretion of B-vitamins by normal adults. Arch Biochem. 1947 Nov;15:187-94. View abstract.
- Rivera JA, González-Cossío T, Flores M, et al. Multiple micronutrient supplementation increases the growth of Mexican infants. Am J Clin Nutr. 2001 Nov;74:657-63. View abstract.
- Roth K.S. Biotin in clinical medicine--a review. Am J Clin Nutr. 1981 Sep;34:1967-74. View abstract.
- Fiume MZ. Cosmetic Ingredient Review Expert Panel. Final report on the safety assessment of biotin. Int J Toxicol. 2001;20 Suppl 4:1-12. View abstract.
- Geohas J, Daly A, Juturu V, et al. Chromium picolinate and biotin combination reduces atherogenic index of plasma in patients with type 2 diabetes mellitus: a placebo-controlled, double-blinded, randomized clinical trial. Am J Med Sci. 2007 Mar;333:145-53. View abstract.
- Ebek, Inc. issues voluntary nationwide recall of Liviro3, a product marketed as a dietary supplement. Ebek Press Release, January 19, 2007. Available at: http://www.fda.gov/oc/po/firmrecalls/ebek01_07.html.
- Singer GM, Geohas J. The effect of chromium picolinate and biotin supplementation on glycemic control in poorly controlled patients with type 2 diabetes mellitus: a placebo-controlled, double-blinded, randomized trial. Diabetes Technol Ther 2006;8:636-43. View abstract.
- Rathman SC, Eisenschenk S, McMahon RJ. The abundance and function of biotin-dependent enzymes are reduced in rats chronically administered carbamazepine. J Nutr 2002;132:3405-10. View abstract.
- Mock DM, Dyken ME. Biotin catabolism is accelerated in adults receiving long-term therapy with anticonvulsants. Neurology 1997;49:1444-7. View abstract.
- Albarracin C, Fuqua B, Evans JL, Goldfine ID. Chromium picolinate and biotin combination improves glucose metabolism in treated, uncontrolled overweight to obese patients with type 2 diabetes. Diabetes Metab Res Rev 2008;24:41-51. View abstract.
- Geohas J, Finch M, Juturu V, et al. Improvement in Fasting Blood Glucose with the Combination of Chromium Picolinate and Biotin in Type 2 Diabetes Mellitus. American Diabetes Association 64th Annual Meeting, June 2004, Orlando, Florida, abstract 191-OR.
- Mock DM, Dyken ME. Biotin deficiency results from long-term therapy with anticonvulsants (abstract). Gastroenterology 1995;108:A740.
- Krause KH, Berlit P, Bonjour JP. Vitamin status in patients on chronic anticonvulsant therapy. Int J Vitam Nutr Res 1982;52:375-85. View abstract.
- Krause KH, Kochen W, Berlit P, Bonjour JP. Excretion of organic acids associated with biotin deficiency in chronic anticonvulsant therapy. Int J Vitam Nutr Res 1984;54:217-22. View abstract.
- Sealey WM, Teague AM, Stratton SL, Mock DM. Smoking accelerates biotin catabolism in women. Am J Clin Nutr 2004;80:932-5. View abstract.
- Mock NI, Malik MI, Stumbo PJ, et al. Increased urinary excretion of 3-hydroxyisovaleric acid and decreased urinary excretion of biotin are sensitive early indicators of decreased status in experimental biotin deficiency. Am J Clin Nutr 1997;65:951-8. View abstract.
- Baez-Saldana A, Zendejas-Ruiz I, Revilla-Monsalve C, et al. Effects of biotin on pyruvate carboxylase, acetyl-CoA carboxylase, propionyl-CoA carboxylase, and markers for glucose and lipid homeostasis in type 2 diabetic patients and nondiabetic subjects. Am J Clin Nutr 2004;79:238-43. View abstract.
- Zempleni J, Mock DM. Bioavailability of biotin given orally to humans in pharmacologic doses. Am J Clin Nutr 1999;69:504-8. View abstract.
- Said HM. Biotin: the forgotten vitamin. Am J Clin Nutr. 2002;75:179-80. View abstract.
- Keipert JA. Oral use of biotin in seborrhoeic dermatitis of infancy: a controlled trial. Med J Aust 1976;1:584-5. View abstract.
- Koutsikos D, Agroyannis B, Tzanatos-Exarchou H. Biotin for diabetic peripheral neuropathy. Biomed Pharmacother 1990;44:511-4. View abstract.
- Coggeshall JC, Heggers JP, Robson MC, et al. Biotin status and plasma glucose in diabetics. Ann N Y Acad Sci 1985;447:389-92.
- Zempleni J, Helm RM, Mock DM. In vivo biotin supplementation at a pharmacologic dose decreases proliferation rates of human peripheral blood mononuclear cells and cytokine release. J Nutr 2001;131:1479-84. View abstract.
- Mock DM, Quirk JG, Mock NI. Marginal biotin deficiency during normal pregnancy. Am J Clin Nutr 2002;75:295-9. View abstract.
- Camacho FM, Garcia-Hernandez MJ. Zinc aspartate, biotin, and clobetasol propionate in the treatment of alopecia areata in childhood. Pediatr Dermatol 1999;16:336-8. View abstract.
- Food and Nutrition Board, Institute of Medicine. Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline . Washington, DC: National Academy Press, 2000. Available at: http://books.nap.edu/books/0309065542/html/.
- Hill MJ. Intestinal flora and endogenous vitamin synthesis. Eur J Cancer Prev 1997;6:S43-5. View abstract.
- Debourdeau PM, Djezzar S, Estival JL, et al. Life-threatening eosinophilic pleuropericardial effusion related to vitamins B5 and H. Ann Pharmacother 2001;35:424-6. View abstract.
- Shils ME, Olson JA, Shike M, Ross AC, eds. Modern Nutrition in Health and Disease. 9th ed. Baltimore, MD: Williams & Wilkins, 1999.
- Brewster MA, Schedewie H. Trimethylaminuria. Ann Clin Lab Sci 1983;13:20-4. View abstract.
- Lininger SW. The Natural Pharmacy. 1st ed. Rocklin, CA: Prima Publishing; 1998.
- Mock DM, Mock NI, Nelson RP, Lombard KA. Disturbances in biotin metabolism in children undergoing long-term anticonvulsant therapy. J Pediatr Gastroentereol Nutr 1998;26:245-50. View abstract.
- Krause KH, Bonjour JP, Berlit P, Kochen W. Biotin status of epileptics. Ann N Y Acad Sci 1985;447:297-313. View abstract.
- Bonjour JP. Biotin in human nutrition. Ann N Y Acad Sci 1985;447:97-104. View abstract.
- Said HM, Redha R, Nylander W. Biotin transport in the human intestine: inhibition by anticonvulsant drugs. Am J Clin Nutr 1989;49:127-31. View abstract.
- Hochman LG, Scher RK, Meyerson MS. Brittle nails: response to daily biotin supplementation. Cutis 1993;51:303-5. View abstract.
- Henry JG, Sobki S, Afafat N. Interference by biotin therapy on measurement of TSH and FT4 by enzyme immunoassay on Boehringer Mannheim ES 700 analyzer. Ann Clin Biochem 1996;33:162-3. View abstract.

