LOCUS       pAM46                   9544 bp ds-DNA     circular     15-DEC-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     CRISPR repair vector for cac::V5
COMMENT     SgfI fragment of pAM45 was cloned into SgfI cut pAM43
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(8545..8562)
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            join(5813..6120,6163..6179)
                     /gene=FBgn0263111
                     /locus_tag="cac"
                     /note="cacophony [Source:FlyBase gene
                     name;Acc:FBgn0263111]"
                     /label=FBgn0263111
                     /ApEinfo_fwdcolor=#f4ff3c
                     /ApEinfo_revcolor=#f4ff3c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            1232..3065
                     /gene=FBgn0263111
                     /locus_tag="cac"
                     /note="cacophony [Source:FlyBase gene
                     name;Acc:FBgn0263111]"
                     /label=FBgn0263111(1)
                     /ApEinfo_label=FBgn0263111
                     /ApEinfo_fwdcolor=#f4ff3c
                     /ApEinfo_revcolor=#f4ff3c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            5813..5907
                     /note="exon_id=FBtr0307321-E30"
                     /label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(3066..3099)
                     /label=LoxP
                     /ApEinfo_fwdcolor=#309c1e
                     /ApEinfo_revcolor=#309c1e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5973..6120,6163..6179)
                     /note="exon_id=FBtr0307325-E30"
                     /label=exon(1)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            join(4335..4367,4369..5006,5009..5042,5044..5107,
                     5109..5288,5290..5458,5460..5535,5537..5555,5557..5559,
                     5561..5579,5581..5617,5620..5620,5622..5622,5624..5624,
                     5626..5628,5630..5630,5632..5812,6180..6202,6204..6488,
                     6490..6651,6653..6784,6786..6836,6838..7138,7140..7518,
                     7520..7660,7662..7952,7954..8048)
                     /gene=FBgn0263111
                     /locus_tag="cac"
                     /note="cacophony [Source:FlyBase gene
                     name;Acc:FBgn0263111]"
                     /label=FBgn0263111(2)
                     /ApEinfo_label=FBgn0263111
                     /ApEinfo_fwdcolor=#f4ff3c
                     /ApEinfo_revcolor=#f4ff3c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5973..6120,6163..6179)
                     /note="exon_id=FBtr0332391-E32"
                     /label=exon(2)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            1232..1252
                     /note="exon_id=FBtr0307321-E27"
                     /label=exon(3)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5973..6120,6163..6179)
                     /note="exon_id=FBtr0307317-E31"
                     /label=exon(4)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(4257..4290)
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=#309c1e
                     /ApEinfo_revcolor=#309c1e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5973..6120,6163..6179)
                     /note="exon_id=FBtr0307315-E32"
                     /label=exon(5)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            1824..2024
                     /note="exon_id=FBtr0307321-E28"
                     /label=exon(6)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5973..6120,6163..6179)
                     /note="exon_id=FBtr0307321-E31"
                     /label=exon(7)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    11..859
                     /label=AmpR
                     /ApEinfo_fwdcolor=#fffa4a
                     /ApEinfo_revcolor=#fffa4a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6109..6120
                     /label=PBM
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            5663..5812
                     /note="exon_id=FBtr0307321-E30"
                     /label=exon(8)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6163..6165
                     /label=STOP
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2262..2284
                     /label=CRISPR site #7 (+) (0 off site targets)
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6121..6162
                     /label=V5-Tag
                     /ApEinfo_fwdcolor=#dd98ff
                     /ApEinfo_revcolor=#dd98ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    901..929
                     /label=amp promoter
                     /ApEinfo_fwdcolor=#feffb1
                     /ApEinfo_revcolor=#feffb1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1912..1932
                     /label=am_131f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3331..4008
                     /label=DsRed
                     /ApEinfo_fwdcolor=#ff0a3a
                     /ApEinfo_revcolor=#ff0a3a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6180..6202,6204..6488,6490..6651,6653..6784,
                     6786..6836,6838..7138,7140..7518,7520..7660,7662..7952,
                     7954..8048)
                     /note="exon_id=FBtr0307325-E30"
                     /label=exon(9)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2591..2611
                     /label=am_132r
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8816..9435
                     /label=pBR322 ori
                     /ApEinfo_fwdcolor=#b6b6b6
                     /ApEinfo_revcolor=#b6b6b6
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4010..4250
                     /label=SV40 3'UTR
                     /ApEinfo_fwdcolor=#ffb6fc
                     /ApEinfo_revcolor=#ffb6fc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3106..3311
                     /label=3xP3 enhancer/hsp70 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5371..5458,5460..5522)
                     /note="exon_id=FBtr0307321-E29"
                     /label=exon(10)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4292..4298
                     /label=SapI (1/4 cutter)
                     /ApEinfo_fwdcolor=#c0c1b7
                     /ApEinfo_revcolor=#c0c1b7
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8071..8077
                     /label=SapI (1/4 cutter)(1)
                     /ApEinfo_label=SapI (1/4 cutter)
                     /ApEinfo_fwdcolor=#c0c1b7
                     /ApEinfo_revcolor=#c0c1b7
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(4299..4334,8055..8070)
                     /label=MCS 3'
                     /ApEinfo_fwdcolor=#74c9ff
                     /ApEinfo_revcolor=#74c9ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3749..3771
                     /label=am_162f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6180..6202,6204..6410)
                     /note="exon_id=FBtr0332391-E32"
                     /label=exon(11)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8049..8054
                     /label=AvrII
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4329..4334
                     /label=AvrII(1)
                     /ApEinfo_label=AvrII
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6480..6488,6490..6651,6653..6784,6786..6836,
                     6838..7138,7140..7518,7520..7660,7662..7952,7954..8048)
                     /note="exon_id=FBtr0332391-E33"
                     /label=exon(12)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6180..6202,6204..6488,6490..6651,6653..6784,
                     6786..6836,6838..7138,7140..7518,7520..7660,7662..7952,
                     7954..8048)
                     /note="exon_id=FBtr0307317-E31"
                     /label=exon(13)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6180..6202,6204..6488,6490..6651,6653..6784,
                     6786..6836,6838..7138,7140..7518,7520..7660,7662..7952,
                     7954..8048)
                     /note="exon_id=FBtr0307315-E32"
                     /label=exon(14)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6977..6999
                     /label=CRISPR site #8 (+)
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6615..6636
                     /label=am_133f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7343..7364
                     /label=am_134r
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6180..6202,6204..6488,6490..6651,6653..6784,
                     6786..6836,6838..7138,7140..7518,7520..7660,7662..7952,
                     7954..8048)
                     /note="exon_id=FBtr0307321-E31"
                     /label=exon(15)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6615..6636
                     /label=am_133f(1)
                     /ApEinfo_label=am_133f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5664..5687
                     /label=am_182f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5058..5082
                     /label=am_181f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4368..4368
                     /label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5007..5007
                     /label=T > A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5008..5008
                     /label=C > T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5043..5043
                     /label=T > C(1)
                     /ApEinfo_label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5108..5108
                     /label=A > T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6203..6203
                     /label=A > T(1)
                     /ApEinfo_label=A > T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6489..6489
                     /label=A > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6652..6652
                     /label=maybe del?
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5289..5289
                     /label=T > C(2)
                     /ApEinfo_label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5459..5459
                     /label=G > A silent
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5536..5536
                     /label=G > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5546..5546
                     /label=delet TCCGATATCCTAGACTC after A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5549..5549
                     /label=delet CTGCCTGCGAGTGGGAGGGATATATGCAA after T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5556..5556
                     /label=A > G(1)
                     /ApEinfo_label=A > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5560..5560
                     /label=G > A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5580..5580
                     /label=A > G(2)
                     /ApEinfo_label=A > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5618..5618
                     /label=T > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5619..5619
                     /label=C > T(1)
                     /ApEinfo_label=C > T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5621..5621
                     /label=T > C(3)
                     /ApEinfo_label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5623..5623
                     /label=T > G(1)
                     /ApEinfo_label=T > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5625..5625
                     /label=G > A(1)
                     /ApEinfo_label=G > A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5629..5629
                     /label=T > G(2)
                     /ApEinfo_label=T > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5631..5631
                     /label=A > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6785..6785
                     /label=A > G(3)
                     /ApEinfo_label=A > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6837..6837
                     /label=T > A(1)
                     /ApEinfo_label=T > A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7139..7139
                     /label=T > C(4)
                     /ApEinfo_label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7519..7519
                     /label=A > T(2)
                     /ApEinfo_label=A > T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7661..7661
                     /label=T > A(2)
                     /ApEinfo_label=T > A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7953..7953
                     /label=T > C(5)
                     /ApEinfo_label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8089..8089
                     /label=T del after T?
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1321..1321
                     /label=TG missing after G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1697..1697
                     /label=AAA missing after A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttcg cgtcatccgc ggtatccaag aggtatttat
     1261 tggctcgaca tccatggcat ttaatcatta actactttca actgactatc gtgtgtgtgt
     1321 gtgtgtgctg gttgtaagcc aaatgatctg gttagggatt cgtcaacatt atcctgttat
     1381 cgagttgtct gtccttgatg ttgcccttaa tcacttcgta tccatatgag atcggcttgc
     1441 aaggggcaca cacagaaaac aaagggactt ggacattcca gggatttttg atctattttg
     1501 tagaaccgta gttatataca tatatacata tatgtctaca attccatagc tattaacgaa
     1561 atcttttaat acaaatatat tatatttcaa taataatttt atcgtttccg ttaagtcggt
     1621 cctctattgt cgtttatcgt ttatctcttc gaaagcaatt tgaaaatcgc aaaaaaccaa
     1681 aatgaaaaaa aaaaaaacaa aacaaaacaa aacaaaagaa gagaagagaa aatatgttgc
     1741 taagatccaa tacgtaaaat gttgtgcccc gacatcctta attattactt ttttttatta
     1801 ttgttgttat atgtacaata taggttcatg gtcagcatcg acaagtccgg cccgttcgcc
     1861 ttcgccttct cgatatggtg gtcatttgtc tcgcagtaaa cgcactcaac tgccttatcc
     1921 cacatatggg acaaccagtc tatgtcaaag atcacgatcg ccgagtcccg ctagacttca
     1981 ggagatgcgt gaacgagaca gacttggtta tgggattgat atgggtacat ccttagcact
     2041 tgttatctaa agtccaacaa gctcaatcaa aaatcaactt taaacaattt aacaaaaaca
     2101 actactcaac ttgttttcta tatatgctag tattttcttc tacacaaatt tcgacttcaa
     2161 ccatctcaat tttaacaaac atcaaagatc agacatgctt actcgtaaat cgttaactcg
     2221 taactcgtat ctcgtaacta acttgtattc gtacatagtt gccaaccatg cttaacatgt
     2281 atacatgtat tgtcatgccc agacgttcaa tcagaaaaca aacagtcaat caatcaatca
     2341 atcaatcaat caatcaatca gccaatcagt caaccataag cttcaacaaa tcgaatagtt
     2401 caaatactct tttcatacat ccaaacacag ctctcaaaaa ttcattcaga tctgcgatct
     2461 accacaccga agaactcatg attatgtgac caactattcg tatttcgtat tcgtactctt
     2521 gttacactag cagcgtttat gttttcattt gtctagaagc catcctcgta acatccacct
     2581 atattataat cacatctcgt atgtatggtg ctgacatgta cagacatatt catccttgtc
     2641 attaaactaa cacactgaat ctctggcaaa tgaactgttg aatagcaata gaggagttta
     2701 aatatatacg atttttatgt caggactact ttcgacggac tcttagtaga acaccaagta
     2761 ctggtcactt tttaaaaaca gcatcgatgt gagagaaatg tgaatatatt atggaacagc
     2821 aatgtgtagg ggtgagataa gtgaacatca tttcctagtc tattccacaa agaaggtgaa
     2881 gttcgtaagc catagtatgt agaacatata tatatatata taaatttttt tttttgttca
     2941 aaattgacct ctgtcagcct gaccagcaca ggataagaca ctgaccaaga atctctattt
     3001 aaagcttgct tcttgttcca aaaaaaacac aaaaaaaaaa acacccagtt actaccacag
     3061 atatgataac ttcgtataat gtatgctata cgaagttatc gtacgggatc taattcaatt
     3121 agagactaat tcaattagag ctaattcaat taggatccaa gcttatcgat ttcgaaccct
     3181 cgaccgccgg agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa
     3241 gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc
     3301 taaacaatcg gctcgaagcc ggtcgccacc atggcctcct ccgaggacgt catcaaggag
     3361 ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag
     3421 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     3481 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag
     3541 gtgtacgtga agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc
     3601 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     3661 tcctccctcc aggacggctc cttcatctac aaggtgaagt tcatcggcgt gaacttcccc
     3721 tccgacggcc ccgtaatgca gaagaagact atgggctggg aggcgtccac cgagcgcctg
     3781 tacccccgcg acggcgtgct gaagggcgag atccacaagg ccctgaagct gaaggacggc
     3841 ggccactacc tggtggagtt caagtccatc tacatggcca agaagcccgt gcagctgccc
     3901 ggctactact acgtggactc caagctggac atcacctccc acaacgagga ctacaccatc
     3961 gtggagcagt acgagcgcgc cgagggccgc caccacctgt tcctgtaggg gccgcgactc
     4021 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     4081 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     4141 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4201 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta accggtataa
     4261 cttcgtataa tgtatgctat acgaagttat agaagagcac tagtaaagat ctccatgcat
     4321 aaggcgcgcc taggcaggca atgttccagt attgtttttt gtaccagctt tgggcggtga
     4381 aatcgactga gctacataca tactcgtcta tgtaatatta taaccagagg aataatttgg
     4441 gttgcagcct tcgggtgttt cagtatctag acgtgtgtgc atatttatct atcttatcta
     4501 tctgtgaatg tgcgtcaata gtattctgca ccatttctta atccatcctt ttcgtttttg
     4561 cataattgat atctctatag atcaaatcga aatcaaaaca gtcacgcatg ttgccatgaa
     4621 caatttagct gccgaccggt cattacccca aatcatccaa ctacttgtgt atatttctct
     4681 caatacatta tatatagcac tgttgttaat gtttcatatt tttctaagtt gtataattct
     4741 gtagggtgct caaataattc cacacgctta tgaacgtaga tacagtgaat gcatttttgg
     4801 catagcaaca atttttaaac aacattaaag tttaatatgt aaactacgtt ctgattggga
     4861 catgttcatc ctttacacaa tatcgtaaaa tcaccattaa atcgtaattg tgcaaaaaat
     4921 attaacttcg agttttgaaa gaacctgtca aataaatctt gtttctataa agtttgtatg
     4981 tgtattttta gagataacta atgaaaatat aaatcgttta acgctaactt tgacctgtac
     5041 ttccagaatt caatagtcct tactgagact taacaaaatc ccactaaatg tcgcacaata
     5101 aaattcatca taaatctggt tacgataaag tctgtctgat tgtcttcagt tattagatag
     5161 ttagtcctaa tctaatccaa atgaacatgt cccattctat attatgtttc atatatttgt
     5221 atgtatgttt taagtccatg ttaatgtcaa tgaagaacga cttaggtaca ctttgttcag
     5281 tatcataaca gcagagtatt tttgcaacga cttatgccct aaactgcact tgttcagcac
     5341 acagggaagt aatttttcga tttcccgcag gtgtaacgca tgtacagcat agttacccaa
     5401 cactggcctc ccgaagagcc ggaatcggaa gacgccttcc tccgactccc agtaaaccat
     5461 caacactgca gctcaagcca accaatatca atttcccgaa gctgaatgcc agtcccacac
     5521 atgtaagtga ctatccatcg agggtaactg catcagagca actgtttgct cataatgagg
     5581 atgagaacta atattcatag gaaaacaaat agaacaagta cagaaaatga ccataattaa
     5641 taatgtttaa tttttccgac agacacacca ctcaacgccc cacagtgttc actcgctacc
     5701 acaccaccgc gacctgctgc gagatccaag ggatatgtac tatagtagta gagaacgcga
     5761 gcgggatcgc gaacgcctta gggatcggga tcgtgatagg gatcgcgatc gcctgcacga
     5821 gtacgacctg cggtacgagt accgggatcg ggaacgagag ttgtacgaac gcgaaagaga
     5881 tcgcgagcgg gaggtcgaaa gagaaaggtg agttggtgct atatagtatt aggagcactc
     5941 aagtaataca tatatgtcct tatgtaccgc agactggaat atatagcacc gctgtcgttt
     6001 gaacaagcgc tggctatggg ccgaacaggt cgtgtactgc catctccagt tctaaacggt
     6061 tttaagccaa agagtggtct gaaccctcga cactccgatt cggatgagga ggattggtgc
     6121 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgTAGcaaag gctttgattg
     6181 cgatcgcgta atcgaaagga tctgatctgg gtatagcatc aggccatttg ctcctctttg
     6241 tcagcaccaa acttagatca caattttgat acgatacgac acagctatca gatacccgaa
     6301 catctgcaaa accttccata cctttgtcca ccagtacacc aacaccaaca ccaacaccaa
     6361 acccaaaccc aaaaccaacg acaacaccaa gatcagcacc tatatcaaag gtatggcacg
     6421 aacgtatcga caccgaaact aggaacaact atatctgata tcaaattggc tggagttagc
     6481 ttacaggagt taagcccgaa tcgacaaccg caatcgcaac agaagcagga gcaacaattg
     6541 catcaggatg gatggctcag atggtaccgg caatcgggtc catacggatc cccagtgatg
     6601 gcatcattgc cggagaccag agcaacatcg ccccactccg atatcgaatt cattagaact
     6661 atgaagagaa gtgaaaccgg aacaactgca ataacaccaa ggacaactaa aactgaagca
     6721 atgatattaa gcgtaccaat tacaataacg tccccattag ctattaaaaa gatatattca
     6781 gatagtgaca ttaattataa taccgaacga ggaactgata atgacagagt ggacgaagca
     6841 ccagctcatc attatcacca gccagaacta actacaccag atctacaaga cgagccacca
     6901 ctgcagcagc atcaccagca ctatccacag tattcgtatc atacgctcta aggaggatag
     6961 gatcaagaac gagcaaccat tatgcagacg ccacaatcca tagttcatag tccagaaact
     7021 agtatccaga atctagaaac tagaaaccag aaaccagaaa ccagatccac taaaagtgca
     7081 actttaattg aaactcaaac tgaaactgaa acgaggtgta aagaatgtga gagtgattct
     7141 attccacgca attggattgg gagtaaacgt ggggagattg taaacagatt agagctacta
     7201 tttgcttaga aataggagta gacaacataa ttttagatat gaatatgatc aagtgtattg
     7261 tacttttttt ttaatgtaag ggcaagtcta gacgaagagc acatcaaaat gaagctaacg
     7321 ataacagaac tcttttcaag cagaacacac atagatctag atcgaaaata tatatatata
     7381 ggaaataaag attgtaggta ctcgcataca ttgtaagtta tctagaattt agctcgagga
     7441 atcggtacgg taatctgcaa gcgtttttga cttgtttata ttaaaagtta gtacttcaat
     7501 tatgtgtatt taaatgcgtc atttatttat ttactcaatg tacataccag ataattgtag
     7561 atatgaaatt gttatttatt ttattctttt gtatgtaaaa tatttaccta cgactactat
     7621 tataaatacc ttcatcagac cgaatcccta tagcgaatat aagcaaaaat tcagcgcaat
     7681 ttagtcctct acggttttaa cattcagttg tgtatttata ctcgagcgga tacttttttt
     7741 ccacgattag tagaagtcca cgcaacatgg caagtccagc atattgaatt caaatccaag
     7801 tccaaaacta gatccaaata actcaattat gaccaaaagc atgtatctgt gtaaagtaag
     7861 catcattcag tgccatttgt ttatacagac tctagctaac ttacaatagc ttgtttatca
     7921 tacatatttg ccttagcgaa tgaaacgttg ttctcatagt tactctgtaa tctgcaatcg
     7981 acgagaggtt tatgcttcta ttaatgcgtt tcgtatgctg aaattagctc ctcgcaaact
     8041 ttccgatcCC TAGGccttct gcagctcgag gctcttccgt caatcgagtc aagggcgaca
     8101 caaaatttat tctaaatgca taataaatac tgataacatc ttatagtttg tattatattt
     8161 tgtattatcg ttgacatgta taattttgat atcaaaaact gattttccct ttattatttt
     8221 cgagatttat tttcttaatt ctctttaaca aactagaaat attgtatata caaaaaatca
     8281 taaataatag atgaatagtt taattatagg tgttcatcaa tcgaaaaagc aacgtatctt
     8341 atttaaagtg cgttgctttt ttctcattta taaggttaaa taattctcat atatcaagca
     8401 aagtgacagg cgcccttaaa tattctgaca aatgctcttt ccctaaactc cccccataaa
     8461 aaaacccgcc gaagcgggtt tttacgttat ttgcggatta acgattactc gttatcagaa
     8521 ccgcccaggg ggcccgagct taagactggc cgtcgtttta caacacagaa agagtttgta
     8581 gaaacgcaaa aaggccatcc gtcaggggcc ttctgcttag tttgatgcct ggcagttccc
     8641 tactctcgcc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8701 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8761 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8821 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8881 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8941 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     9001 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     9061 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     9121 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9181 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9241 agtggtgggc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9301 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9361 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9421 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg cgcgtaactc
     9481 acgttaaggg attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc
     9541 tttt     
//
