LOCUS       pAM46                  10225 bp ds-DNA     circular     15-DEC-2016
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     CRISPR repair vector for cac::EGFP
COMMENT     SgfI 1kb fragment of pAM44 was cloned into SgfI cut pAM43
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(9226..9243)
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            join(5813..6120,6844..6860)
                     /gene=FBgn0263111
                     /locus_tag="cac"
                     /note="cacophony [Source:FlyBase gene
                     name;Acc:FBgn0263111]"
                     /label=FBgn0263111
                     /ApEinfo_fwdcolor=#f4ff3c
                     /ApEinfo_revcolor=#f4ff3c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            1232..3065
                     /gene=FBgn0263111
                     /locus_tag="cac"
                     /note="cacophony [Source:FlyBase gene
                     name;Acc:FBgn0263111]"
                     /label=FBgn0263111(1)
                     /ApEinfo_label=FBgn0263111
                     /ApEinfo_fwdcolor=#f4ff3c
                     /ApEinfo_revcolor=#f4ff3c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            5813..5907
                     /note="exon_id=FBtr0307321-E30"
                     /label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(3066..3099)
                     /label=LoxP
                     /ApEinfo_fwdcolor=#309c1e
                     /ApEinfo_revcolor=#309c1e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5973..6120,6844..6860)
                     /note="exon_id=FBtr0307325-E30"
                     /label=exon(1)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            join(4335..4367,4369..5006,5009..5042,5044..5107,
                     5109..5288,5290..5458,5460..5535,5537..5555,5557..5559,
                     5561..5579,5581..5617,5620..5620,5622..5622,5624..5624,
                     5626..5628,5630..5630,5632..5812,6861..6883,6885..7169,
                     7171..7332,7334..7465,7467..7517,7519..7819,7821..8199,
                     8201..8341,8343..8633,8635..8729)
                     /gene=FBgn0263111
                     /locus_tag="cac"
                     /note="cacophony [Source:FlyBase gene
                     name;Acc:FBgn0263111]"
                     /label=FBgn0263111(2)
                     /ApEinfo_label=FBgn0263111
                     /ApEinfo_fwdcolor=#f4ff3c
                     /ApEinfo_revcolor=#f4ff3c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5973..6120,6844..6860)
                     /note="exon_id=FBtr0332391-E32"
                     /label=exon(2)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            1232..1252
                     /note="exon_id=FBtr0307321-E27"
                     /label=exon(3)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5973..6120,6844..6860)
                     /note="exon_id=FBtr0307317-E31"
                     /label=exon(4)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_recomb     complement(4257..4290)
                     /label=LoxP(1)
                     /ApEinfo_label=LoxP
                     /ApEinfo_fwdcolor=#309c1e
                     /ApEinfo_revcolor=#309c1e
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5973..6120,6844..6860)
                     /note="exon_id=FBtr0307315-E32"
                     /label=exon(5)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            1824..2024
                     /note="exon_id=FBtr0307321-E28"
                     /label=exon(6)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5973..6120,6844..6860)
                     /note="exon_id=FBtr0307321-E31"
                     /label=exon(7)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    11..859
                     /label=AmpR
                     /ApEinfo_fwdcolor=#fffa4a
                     /ApEinfo_revcolor=#fffa4a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6109..6120
                     /label=PBM
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            5663..5812
                     /note="exon_id=FBtr0307321-E30"
                     /label=exon(8)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6844..6846
                     /label=STOP
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2262..2284
                     /label=CRISPR site #7 (+) (0 off site targets)
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6121..6843
                     /label=EGFP (S65T)
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    901..929
                     /label=amp promoter
                     /ApEinfo_fwdcolor=#feffb1
                     /ApEinfo_revcolor=#feffb1
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6174..6198
                     /label=am_29f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1912..1932
                     /label=am_131f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3331..4008
                     /label=DsRed
                     /ApEinfo_fwdcolor=#ff0a3a
                     /ApEinfo_revcolor=#ff0a3a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6861..6883,6885..7169,7171..7332,7334..7465,
                     7467..7517,7519..7819,7821..8199,8201..8341,8343..8633,
                     8635..8729)
                     /note="exon_id=FBtr0307325-E30"
                     /label=exon(9)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2591..2611
                     /label=am_132r
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9497..10116
                     /label=pBR322 ori
                     /ApEinfo_fwdcolor=#b6b6b6
                     /ApEinfo_revcolor=#b6b6b6
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4010..4250
                     /label=SV40 3'UTR
                     /ApEinfo_fwdcolor=#ffb6fc
                     /ApEinfo_revcolor=#ffb6fc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3106..3311
                     /label=3xP3 enhancer/hsp70 promoter
                     /ApEinfo_fwdcolor=#00ffff
                     /ApEinfo_revcolor=#00ffff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(5371..5458,5460..5522)
                     /note="exon_id=FBtr0307321-E29"
                     /label=exon(10)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4292..4298
                     /label=SapI (1/4 cutter)
                     /ApEinfo_fwdcolor=#c0c1b7
                     /ApEinfo_revcolor=#c0c1b7
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8752..8758
                     /label=SapI (1/4 cutter)(1)
                     /ApEinfo_label=SapI (1/4 cutter)
                     /ApEinfo_fwdcolor=#c0c1b7
                     /ApEinfo_revcolor=#c0c1b7
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(4299..4334,8736..8751)
                     /label=MCS 3'
                     /ApEinfo_fwdcolor=#74c9ff
                     /ApEinfo_revcolor=#74c9ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3749..3771
                     /label=am_162f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6861..6883,6885..7091)
                     /note="exon_id=FBtr0332391-E32"
                     /label=exon(11)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8730..8735
                     /label=AvrII
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4329..4334
                     /label=AvrII(1)
                     /ApEinfo_label=AvrII
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(7161..7169,7171..7332,7334..7465,7467..7517,
                     7519..7819,7821..8199,8201..8341,8343..8633,8635..8729)
                     /note="exon_id=FBtr0332391-E33"
                     /label=exon(12)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6861..6883,6885..7169,7171..7332,7334..7465,
                     7467..7517,7519..7819,7821..8199,8201..8341,8343..8633,
                     8635..8729)
                     /note="exon_id=FBtr0307317-E31"
                     /label=exon(13)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6861..6883,6885..7169,7171..7332,7334..7465,
                     7467..7517,7519..7819,7821..8199,8201..8341,8343..8633,
                     8635..8729)
                     /note="exon_id=FBtr0307315-E32"
                     /label=exon(14)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7658..7680
                     /label=CRISPR site #8 (+)
                     /ApEinfo_fwdcolor=#408080
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7296..7317
                     /label=am_133f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8024..8045
                     /label=am_134r
                     /ApEinfo_fwdcolor=#808000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     exon            join(6861..6883,6885..7169,7171..7332,7334..7465,
                     7467..7517,7519..7819,7821..8199,8201..8341,8343..8633,
                     8635..8729)
                     /note="exon_id=FBtr0307321-E31"
                     /label=exon(15)
                     /ApEinfo_label=exon
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7296..7317
                     /label=am_133f(1)
                     /ApEinfo_label=am_133f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5664..5687
                     /label=am_182f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5058..5082
                     /label=am_181f
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4368..4368
                     /label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5007..5007
                     /label=T > A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5008..5008
                     /label=C > T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5043..5043
                     /label=T > C(1)
                     /ApEinfo_label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5108..5108
                     /label=A > T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6884..6884
                     /label=A > T(1)
                     /ApEinfo_label=A > T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7170..7170
                     /label=A > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7333..7333
                     /label=maybe del?
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5289..5289
                     /label=T > C(2)
                     /ApEinfo_label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5459..5459
                     /label=G > A silent
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5536..5536
                     /label=G > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5546..5546
                     /label=delet TCCGATATCCTAGACTC after A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5549..5549
                     /label=delet CTGCCTGCGAGTGGGAGGGATATATGCAA after T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5556..5556
                     /label=A > G(1)
                     /ApEinfo_label=A > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5560..5560
                     /label=G > A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5580..5580
                     /label=A > G(2)
                     /ApEinfo_label=A > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5618..5618
                     /label=T > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5619..5619
                     /label=C > T(1)
                     /ApEinfo_label=C > T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5621..5621
                     /label=T > C(3)
                     /ApEinfo_label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5623..5623
                     /label=T > G(1)
                     /ApEinfo_label=T > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5625..5625
                     /label=G > A(1)
                     /ApEinfo_label=G > A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5629..5629
                     /label=T > G(2)
                     /ApEinfo_label=T > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5631..5631
                     /label=A > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7466..7466
                     /label=A > G(3)
                     /ApEinfo_label=A > G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7518..7518
                     /label=T > A(1)
                     /ApEinfo_label=T > A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7820..7820
                     /label=T > C(4)
                     /ApEinfo_label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8200..8200
                     /label=A > T(2)
                     /ApEinfo_label=A > T
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8342..8342
                     /label=T > A(2)
                     /ApEinfo_label=T > A
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8634..8634
                     /label=T > C(5)
                     /ApEinfo_label=T > C
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8770..8770
                     /label=T del after T?
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1321..1321
                     /label=TG missing after G
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=green
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     misc_feature    1697..1697
                     /label=AAA missing after A
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ORIGIN
        1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
       61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
      181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      481 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
      901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
      961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
     1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
     1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     1201 cccgggctaa ttatggggtg tcgcccttcg cgtcatccgc ggtatccaag aggtatttat
     1261 tggctcgaca tccatggcat ttaatcatta actactttca actgactatc gtgtgtgtgt
     1321 gtgtgtgctg gttgtaagcc aaatgatctg gttagggatt cgtcaacatt atcctgttat
     1381 cgagttgtct gtccttgatg ttgcccttaa tcacttcgta tccatatgag atcggcttgc
     1441 aaggggcaca cacagaaaac aaagggactt ggacattcca gggatttttg atctattttg
     1501 tagaaccgta gttatataca tatatacata tatgtctaca attccatagc tattaacgaa
     1561 atcttttaat acaaatatat tatatttcaa taataatttt atcgtttccg ttaagtcggt
     1621 cctctattgt cgtttatcgt ttatctcttc gaaagcaatt tgaaaatcgc aaaaaaccaa
     1681 aatgaaaaaa aaaaaaacaa aacaaaacaa aacaaaagaa gagaagagaa aatatgttgc
     1741 taagatccaa tacgtaaaat gttgtgcccc gacatcctta attattactt ttttttatta
     1801 ttgttgttat atgtacaata taggttcatg gtcagcatcg acaagtccgg cccgttcgcc
     1861 ttcgccttct cgatatggtg gtcatttgtc tcgcagtaaa cgcactcaac tgccttatcc
     1921 cacatatggg acaaccagtc tatgtcaaag atcacgatcg ccgagtcccg ctagacttca
     1981 ggagatgcgt gaacgagaca gacttggtta tgggattgat atgggtacat ccttagcact
     2041 tgttatctaa agtccaacaa gctcaatcaa aaatcaactt taaacaattt aacaaaaaca
     2101 actactcaac ttgttttcta tatatgctag tattttcttc tacacaaatt tcgacttcaa
     2161 ccatctcaat tttaacaaac atcaaagatc agacatgctt actcgtaaat cgttaactcg
     2221 taactcgtat ctcgtaacta acttgtattc gtacatagtt gccaaccatg cttaacatgt
     2281 atacatgtat tgtcatgccc agacgttcaa tcagaaaaca aacagtcaat caatcaatca
     2341 atcaatcaat caatcaatca gccaatcagt caaccataag cttcaacaaa tcgaatagtt
     2401 caaatactct tttcatacat ccaaacacag ctctcaaaaa ttcattcaga tctgcgatct
     2461 accacaccga agaactcatg attatgtgac caactattcg tatttcgtat tcgtactctt
     2521 gttacactag cagcgtttat gttttcattt gtctagaagc catcctcgta acatccacct
     2581 atattataat cacatctcgt atgtatggtg ctgacatgta cagacatatt catccttgtc
     2641 attaaactaa cacactgaat ctctggcaaa tgaactgttg aatagcaata gaggagttta
     2701 aatatatacg atttttatgt caggactact ttcgacggac tcttagtaga acaccaagta
     2761 ctggtcactt tttaaaaaca gcatcgatgt gagagaaatg tgaatatatt atggaacagc
     2821 aatgtgtagg ggtgagataa gtgaacatca tttcctagtc tattccacaa agaaggtgaa
     2881 gttcgtaagc catagtatgt agaacatata tatatatata taaatttttt tttttgttca
     2941 aaattgacct ctgtcagcct gaccagcaca ggataagaca ctgaccaaga atctctattt
     3001 aaagcttgct tcttgttcca aaaaaaacac aaaaaaaaaa acacccagtt actaccacag
     3061 atatgataac ttcgtataat gtatgctata cgaagttatc gtacgggatc taattcaatt
     3121 agagactaat tcaattagag ctaattcaat taggatccaa gcttatcgat ttcgaaccct
     3181 cgaccgccgg agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa
     3241 gcaaagtgaa cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc
     3301 taaacaatcg gctcgaagcc ggtcgccacc atggcctcct ccgaggacgt catcaaggag
     3361 ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt cgagatcgag
     3421 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     3481 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttccagta cggctccaag
     3541 gtgtacgtga agcaccccgc cgacatcccc gactacaaga agctgtcctt ccccgagggc
     3601 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     3661 tcctccctcc aggacggctc cttcatctac aaggtgaagt tcatcggcgt gaacttcccc
     3721 tccgacggcc ccgtaatgca gaagaagact atgggctggg aggcgtccac cgagcgcctg
     3781 tacccccgcg acggcgtgct gaagggcgag atccacaagg ccctgaagct gaaggacggc
     3841 ggccactacc tggtggagtt caagtccatc tacatggcca agaagcccgt gcagctgccc
     3901 ggctactact acgtggactc caagctggac atcacctccc acaacgagga ctacaccatc
     3961 gtggagcagt acgagcgcgc cgagggccgc caccacctgt tcctgtaggg gccgcgactc
     4021 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     4081 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     4141 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4201 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta accggtataa
     4261 cttcgtataa tgtatgctat acgaagttat agaagagcac tagtaaagat ctccatgcat
     4321 aaggcgcgcc taggcaggca atgttccagt attgtttttt gtaccagctt tgggcggtga
     4381 aatcgactga gctacataca tactcgtcta tgtaatatta taaccagagg aataatttgg
     4441 gttgcagcct tcgggtgttt cagtatctag acgtgtgtgc atatttatct atcttatcta
     4501 tctgtgaatg tgcgtcaata gtattctgca ccatttctta atccatcctt ttcgtttttg
     4561 cataattgat atctctatag atcaaatcga aatcaaaaca gtcacgcatg ttgccatgaa
     4621 caatttagct gccgaccggt cattacccca aatcatccaa ctacttgtgt atatttctct
     4681 caatacatta tatatagcac tgttgttaat gtttcatatt tttctaagtt gtataattct
     4741 gtagggtgct caaataattc cacacgctta tgaacgtaga tacagtgaat gcatttttgg
     4801 catagcaaca atttttaaac aacattaaag tttaatatgt aaactacgtt ctgattggga
     4861 catgttcatc ctttacacaa tatcgtaaaa tcaccattaa atcgtaattg tgcaaaaaat
     4921 attaacttcg agttttgaaa gaacctgtca aataaatctt gtttctataa agtttgtatg
     4981 tgtattttta gagataacta atgaaaatat aaatcgttta acgctaactt tgacctgtac
     5041 ttccagaatt caatagtcct tactgagact taacaaaatc ccactaaatg tcgcacaata
     5101 aaattcatca taaatctggt tacgataaag tctgtctgat tgtcttcagt tattagatag
     5161 ttagtcctaa tctaatccaa atgaacatgt cccattctat attatgtttc atatatttgt
     5221 atgtatgttt taagtccatg ttaatgtcaa tgaagaacga cttaggtaca ctttgttcag
     5281 tatcataaca gcagagtatt tttgcaacga cttatgccct aaactgcact tgttcagcac
     5341 acagggaagt aatttttcga tttcccgcag gtgtaacgca tgtacagcat agttacccaa
     5401 cactggcctc ccgaagagcc ggaatcggaa gacgccttcc tccgactccc agtaaaccat
     5461 caacactgca gctcaagcca accaatatca atttcccgaa gctgaatgcc agtcccacac
     5521 atgtaagtga ctatccatcg agggtaactg catcagagca actgtttgct cataatgagg
     5581 atgagaacta atattcatag gaaaacaaat agaacaagta cagaaaatga ccataattaa
     5641 taatgtttaa tttttccgac agacacacca ctcaacgccc cacagtgttc actcgctacc
     5701 acaccaccgc gacctgctgc gagatccaag ggatatgtac tatagtagta gagaacgcga
     5761 gcgggatcgc gaacgcctta gggatcggga tcgtgatagg gatcgcgatc gcctgcacga
     5821 gtacgacctg cggtacgagt accgggatcg ggaacgagag ttgtacgaac gcgaaagaga
     5881 tcgcgagcgg gaggtcgaaa gagaaaggtg agttggtgct atatagtatt aggagcactc
     5941 aagtaataca tatatgtcct tatgtaccgc agactggaat atatagcacc gctgtcgttt
     6001 gaacaagcgc tggctatggg ccgaacaggt cgtgtactgc catctccagt tctaaacggt
     6061 tttaagccaa agagtggtct gaaccctcga cactccgatt cggatgagga ggattggtgc
     6121 ATGGCTCATA GGAGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA
     6181 TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA
     6241 ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCATGG
     6301 CCAACACTTG TCACTACTTT GACTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAC
     6361 ATGAAACAGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA GGAAAGAACT
     6421 ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT TGAAGGTGAT
     6481 ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT
     6541 GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA
     6601 AAGAATGGAA TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA
     6661 CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC
     6721 AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC
     6781 ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA TGAACTATAC
     6841 AAATAGcaaa ggctttgatt gcgatcgcgt aatcgaaagg atctgatctg ggtatagcat
     6901 caggccattt gctcctcttt gtcagcacca aacttagatc acaattttga tacgatacga
     6961 cacagctatc agatacccga acatctgcaa aaccttccat acctttgtcc accagtacac
     7021 caacaccaac accaacacca aacccaaacc caaaaccaac gacaacacca agatcagcac
     7081 ctatatcaaa ggtatggcac gaacgtatcg acaccgaaac taggaacaac tatatctgat
     7141 atcaaattgg ctggagttag cttacaggag ttaagcccga atcgacaacc gcaatcgcaa
     7201 cagaagcagg agcaacaatt gcatcaggat ggatggctca gatggtaccg gcaatcgggt
     7261 ccatacggat ccccagtgat ggcatcattg ccggagacca gagcaacatc gccccactcc
     7321 gatatcgaat tcattagaac tatgaagaga agtgaaaccg gaacaactgc aataacacca
     7381 aggacaacta aaactgaagc aatgatatta agcgtaccaa ttacaataac gtccccatta
     7441 gctattaaaa agatatattc agatagtgac attaattata ataccgaacg aggaactgat
     7501 aatgacagag tggacgaagc accagctcat cattatcacc agccagaact aactacacca
     7561 gatctacaag acgagccacc actgcagcag catcaccagc actatccaca gtattcgtat
     7621 catacgctct aaggaggata ggatcaagaa cgagcaacca ttatgcagac gccacaatcc
     7681 atagttcata gtccagaaac tagtatccag aatctagaaa ctagaaacca gaaaccagaa
     7741 accagatcca ctaaaagtgc aactttaatt gaaactcaaa ctgaaactga aacgaggtgt
     7801 aaagaatgtg agagtgattc tattccacgc aattggattg ggagtaaacg tggggagatt
     7861 gtaaacagat tagagctact atttgcttag aaataggagt agacaacata attttagata
     7921 tgaatatgat caagtgtatt gtactttttt tttaatgtaa gggcaagtct agacgaagag
     7981 cacatcaaaa tgaagctaac gataacagaa ctcttttcaa gcagaacaca catagatcta
     8041 gatcgaaaat atatatatat aggaaataaa gattgtaggt actcgcatac attgtaagtt
     8101 atctagaatt tagctcgagg aatcggtacg gtaatctgca agcgtttttg acttgtttat
     8161 attaaaagtt agtacttcaa ttatgtgtat ttaaatgcgt catttattta tttactcaat
     8221 gtacatacca gataattgta gatatgaaat tgttatttat tttattcttt tgtatgtaaa
     8281 atatttacct acgactacta ttataaatac cttcatcaga ccgaatccct atagcgaata
     8341 taagcaaaaa ttcagcgcaa tttagtcctc tacggtttta acattcagtt gtgtatttat
     8401 actcgagcgg atactttttt tccacgatta gtagaagtcc acgcaacatg gcaagtccag
     8461 catattgaat tcaaatccaa gtccaaaact agatccaaat aactcaatta tgaccaaaag
     8521 catgtatctg tgtaaagtaa gcatcattca gtgccatttg tttatacaga ctctagctaa
     8581 cttacaatag cttgtttatc atacatattt gccttagcga atgaaacgtt gttctcatag
     8641 ttactctgta atctgcaatc gacgagaggt ttatgcttct attaatgcgt ttcgtatgct
     8701 gaaattagct cctcgcaaac tttccgatcC CTAGGccttc tgcagctcga ggctcttccg
     8761 tcaatcgagt caagggcgac acaaaattta ttctaaatgc ataataaata ctgataacat
     8821 cttatagttt gtattatatt ttgtattatc gttgacatgt ataattttga tatcaaaaac
     8881 tgattttccc tttattattt tcgagattta ttttcttaat tctctttaac aaactagaaa
     8941 tattgtatat acaaaaaatc ataaataata gatgaatagt ttaattatag gtgttcatca
     9001 atcgaaaaag caacgtatct tatttaaagt gcgttgcttt tttctcattt ataaggttaa
     9061 ataattctca tatatcaagc aaagtgacag gcgcccttaa atattctgac aaatgctctt
     9121 tccctaaact ccccccataa aaaaacccgc cgaagcgggt ttttacgtta tttgcggatt
     9181 aacgattact cgttatcaga accgcccagg gggcccgagc ttaagactgg ccgtcgtttt
     9241 acaacacaga aagagtttgt agaaacgcaa aaaggccatc cgtcaggggc cttctgctta
     9301 gtttgatgcc tggcagttcc ctactctcgc cttccgcttc ctcgctcact gactcgctgc
     9361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9901 gcggtgctac agagttcttg aagtggtggg ctaactacgg ctacactaga agaacagtat
     9961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10141 ggaacgacgc gcgcgtaact cacgttaagg gattttggtc atgagcttgc gccgtcccgt
    10201 caagtcagcg taatgctctg ctttt   
//
