Optimisation of HTT-HAP40 purification using heparin affinity chromatography (2019/01/14)
Project - Huntingtin structure-function open lab notebook.
Experiment - Optimisation of HTT-HAP40 purification using heparin affinity chromatography.
Aims - The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin. To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems will be carried out using heparin and FLAG affinity chromatography.
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