ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF RP HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF THIAMINE AND CAFFEINE IN TABLET DOSAGE FORM

The main objective of the study is to develop a simple, accurate and explicit RP-HPLC method for the simultaneous determination of Thiamine and Caffeine in tablet dosage form. An Agilent C18, 25 cm x 4.6mm X 5 μm column is used for the estrangement of drugs by a mobile phase consisting of water : methanol : ACN mixture in the ratio of 68:29:03 v/v. The flow rate maintained was 1.0 mL/min and the wavelength used for detection was 275 nm. The linearity was observed in the range of 10-200μg/ml for Thiamine (TH) and Caffeine (CF) with a correlation coefficient of 0.9995 and 0.9992 respectively. The mean percentage recoveries for 80%, 100% and 120% accuracy were found to be 101.7% ± 2.09, 100% ± 2.49 and 101.5% ± 1.61 respectively for TH and CF. Linearity, accuracy, precision and robustness parameters were estimated for validation. The developed method was simple, precise, less time consuming with accurate results can be utilized in the routine analysis and quantification of TH and CF tablets.