Data/
├── Data Fieldwork 2024/
│ ├── Processed/
│ │ ├── all_substrates_temperatures.xlsx
│ │ ├── diffusa_temperature.xlsx
│ │ ├── hatching_success_and_mortality_2024.xlsx
│ │ ├── leaf_temperature.xlsx
│ │ ├── mortality_data_2021.csv
│ │ └── rainfall_2024.xlsx
│ └── Raw/
│ └── oviposition_substrates_2024.xlsx
└── Data Fieldwork 2025/
└── Processed/
├── devrate_2025_analysis.xlsx
├── egg_survival_matrix.xlsx
├── hatch_success_2025.xlsx
└── Two_choice_matrix.xlsx
Figures/
R_code/
└── code_Ovi_site_E.prosoblepon.qmd
Data Fieldwork 2024 — Processed
1.1 all_substrates_temperatures.xlsx
Temperature and humidity data for all substrate types of the monitored clutches in 2024.
- date Date (mm/dd/yyyy) and hour of the log.
- temp Micro-environmental temperature of the substrate where the clutch was found.
- humid Micro-environmental humidity of the substrate where the clutch was found.
- datalog Identifier of the data logger used for the measurement.
- ClutchID Unique identifier for the clutch.
- susbstrate Type of substrate (following Goyes Vallejos et al. 2024).
1.2 diffusa_temperature.xlsx
Humidity and temperature specifically for monitored clutches on S. diffusa in 2024.
- Hour_date_SellDiff Date (mm/dd/yyyy) and hour of the log.
- SellDif_Micro_temp Micro-environmental temperature on the substrate.
- SellDif_Micro_hum Micro-environmental humidity on the substrate.
- Datalogger Identifier of the data logger used.
- Clutch Unique identifier for the clutch.
1.3 hatching_success_and_mortality_2024.xlsx
Combined data for hatching success and mortality in egg clutches (2024).
- Clutch_size Number of eggs per clutch.
- Substrate Categorisation of the substrates used for oviposition.
- Predation, Dry, Fungal, Failed, Rain, Unknown Counts of eggs dead per mortality source. Predation = embryos predated; Dry = desiccated; Fungal = fungal infections; Rain = rain stripped; Unknown = uncertain cause.
- Num_hatched Total number of tadpoles successfully hatched per clutch.
- Num_died Total number of eggs that died during development.
- Prop.hatched Proportion of eggs hatched (Num_hatched / Clutch_size).
- Prop.mortality Proportion of eggs dead (Num_died / Clutch_size).
1.4 leaf_temperature.xlsx
Humidity and temperature specifically for monitored clutches on leaves in 2024.
- Date Date (mm/dd/yyyy) and hour of the log.
- Temperature Micro-environmental temperature on the substrate.
- Humidity Micro-environmental humidity on the substrate.
- Datalog Identifier of the data logger used.
- ClutchID Unique identifier for the clutch.
- Susbstrate Type of substrate (following Goyes Vallejos et al. 2024). In this case, leaves only.
1.5 mortality_data_2021.csv
Substrate types and mortality sources for egg clutches from Goyes Vallejos et al. (2024) collected in 2021 along the same two transects of the Botanical Garden.
- clutch_size Number of eggs per clutch.
- substrate_type Categorisation of the substrates used for oviposition.
- predation, dry, fungal, failed, rain, unknown Counts of eggs dead per mortality source (same definitions as 1.3, lowercase).
- num_hatched Total number of tadpoles successfully hatched per clutch.
- num_died Total number of eggs that died during development.
- prop.hatched Proportion of eggs hatched (num_hatched / clutch_size).
- prop.mortality Proportion of eggs dead (num_died / clutch_size).
1.6 rainfall_2024.xlsx
Rainfall data and surface temperature for the monitored clutches in 2024.
- transect Transect where the clutch was found.
- substrate Categorisation of the substrates used for oviposition (following Goyes Vallejos et al. 2024).
- day_rain Daily rainfall received on the clutch (mm in the last 24 hours).
Data Fieldwork 2024 — Raw
This directory (…/Data Fieldwork 2024/Raw) contains raw, unprocessed observations from the 2024 field season.
2.1 oviposition_substrates_2024.xlsx
All clutches found in 2024, including oviposition sites and physical/spatial variables measured during searches.
- habitat Category indicating disturbed, secondary, or primary forest. (Note: Only clutches in the disturbed area, Botanical Garden OTS, were used for analyses.)
- transect Name of the surveyed transect.
- location Approximate spatial location where the clutch was found.
- substrate General specification of the physical substrate used for oviposition.
- substrate_type Categorisation of the substrates used for oviposition (following Goyes Vallejos et al. 2024).
- stage_found Developmental stage of the clutches at time of encounter, following Gosner (1960).
- clutch_size Total number of eggs per clutch.
- neighbor_clutches Count of other clutches found nearby within a 20 cm radius.
- distance_water_cm Horizontal distance (cm) from the clutch to the border of the creek.
- height_from_soil_cm Vertical distance (cm) of the clutch location relative to the soil.
- monitored Indicates ('Y') if clutches were found at Gosner stage prior to 18 and subsequently monitored daily.
Data Fieldwork 2025 — Processed
This directory (…/Data Fieldwork 2025/Processed) contains processed data from the 2025 two-choice experiments.
3.1 devrate_2025_analysis.xlsx
Developmental data per clutch for the 2025 choice experiments.
- pair_clutchID Unique identifier for the clutch.
- substrate Substrate type used (either leaf or S. diffusa).
- treatment Type of experiment (either no-choice or two-choice).
- gosnerStage Gosner developmental stage for the monitored day. Dead clutches coded as 999.
- daysfromOvip Number of days elapsed since oviposition.
- earlydead Indicates if the clutch died before reaching hatching competency (Y/N).
3.2 egg_survival_matrix.xlsx
Wide-format matrix for the Kaplan–Meier analyses of embryos in 2025.
- clutchsize Total number of eggs in the clutch.
- substrate Substrate used in the experiment (either S. diffusa or leaf).
- egg Identifies an individual egg within a clutch (development tracked per embryo).
- stageday0, stageday1, stageday2, … Embryo developmental stage (Gosner 1960) on each day since oviposition. Coded 25 at hatching competency (and until hatched, then 1). Coded 0 on the day of death.
3.3 hatch_success_2025.xlsx
Hatching success and mortality sources for both experiments in 2025 (two-choice and no-choice).
- clutch_ID Unique identifier for the clutch.
- substrate Substrate type (either S. diffusa or leaf).
- treatment Type of experiment (either two-choice or no-choice).
- clutchSize Total number of eggs per clutch.
- predated, fungi, non_dev, rainStrip, dehydrated, unknown Counts of dead embryos per mortality category, following Warkentin (2000) and Goyes Vallejos & Ramirez-Soto (2020).
- hatched Total number of eggs successfully hatched.
- hatching_onset Hatching start day (days since oviposition).
- hatching_offset Hatching completion day (days since oviposition).
- totaldead Total number of dead embryos in the clutch.
- hatchSuccess Proportion of successfully hatched eggs (hatched / clutchSize).
3.4 Two_choice_matrix.xlsx
Log of two-choice experiments in 2025, indicating the substrate preferred by females for oviposition.
- hourStart Sampling start time.
- hourEnd Sampling end time.
- hourFound Time when the pair in amplexus was spotted.
- transect Transect in which the pair was found.
- pair_clutchID Unique ID for the pair and resulting clutch.
- clutchSize Number of eggs per clutch.
- substrateChosen Substrate selected by the female for oviposition.
- cage Identifier of the cage used.