Introduction: DNA metabarcoding is currently under intense scrutiny for its future implementation in biodiversity monitoring and ecological assessment, particularly within the EU Water Framework Directive (WFD). However, before metabarcoding can be deployed for WFD reporting, challenges such as the incompleteness of reference databases need to be addressed. In this context, this study presents the results of a national barcoding project (FRESHBAR) focusing on benthic freshwater diatoms of Sweden, a key organism group regarding both ecology and environmental assessment. One major goal of the project was the publication and vouchering of all data, material, and results. Methods: A total of 312 diatom cultures were established, with a focus on oligotrophic and acid habitats. The cultures were sequenced for two barcodes (rbcL and 18SV4) and identified using light microscopy; selected strains were also studied by scanning electron microscopy. All data, including sampling metadata, barcode sequences, images, and voucher material, were published following the FAIR principles. Some cultures were archived in diatom culture collections. Results: Almost all strains were successfully sequenced and identified as 51 taxa across 17 genera, with the highest diversity in the genera Eunotia and Fragilaria. An attempt was also made to identify all taxa at the species level. Approximately half of the taxa were new to Diat.barcode, a curated diatom reference database. Conclusion: FRESHBAR represents the first large-scale effort to generate barcode reference sequences for Nordic benthic freshwater diatoms. As all data are publicly available, the added sequences and morphological information will help identify all taxa properly to the species level, unravel taxon relationships within diatoms, and improve reference databases for environmental monitoring and research.
", "publication_date": "2026-05-26", "publisher": "Pensoft Publishers", "references": [ { "reference": "Abarca N, Jahn R, Zimmermann J, Enke N (2014) Does the cosmopolitan diatom Gomphonema parvulum (K\u00fctzing) K\u00fctzing have a biogeography? PLOS ONE 9: e86885. https://doi.org/10.1371/journal.pone.0086885" }, { "reference": "Abarca N, Zimmermann J, Kusber W-H, Mora D, Van AT, Skibbe O, Jahn R (2020) Defining the core group of the genus Gomphonema Ehrenberg with molecular and morphological methods. Botany Letters 167: 114\u2013159. https://doi.org/10.1080/23818107.2019.1694980" }, { "reference": "\u00c1cs \u00c9, Korponai JL, Kiss KT, B\u00edr\u00f3 T, Duleba M, Grigorszky I, Szekeres J, Vadkerti E, Somlyai I (2025) Integration of morphology-based and molecular techniques to study the most significant diatom order (Thalassiosirales) of phytoplankton in the River Danube. Freshwater Biology 70: e14360. https://doi.org/10.1111/fwb.14360" }, { "reference": "Apoth\u00e9loz\u2010Perret\u2010Gentil L, Bouchez A, Cordier T, Cordonier A, Gu\u00e9guen J, Rimet F, Vasselon V, Pawlowski J (2020) Monitoring the ecological status of rivers with diatom eDNA metabarcoding: A comparison of taxonomic markers and analytical approaches for the inference of a molecular diatom index. Molecular Ecology 30: 2959\u20132968. https://doi.org/10.1111/mec.15646" }, { "reference": "Bailet B, Bouchez A, Franc A, Frigerio J-M, Keck F, Karjalainen S-M, Rimet F, Schneider S, Kahlert M (2019) Molecular versus morphological data for benthic diatoms biomonitoring in Northern Europe freshwater and consequences for ecological status. Metabarcoding and Metagenomics 3: e34002. https://doi.org/10.3897/mbmg.3.34002" }, { "reference": "Bailet B, Apotheloz-Perret-Gentil L, Baricevic A, Chonova T, Franc A, Frigerio J-M, Kelly M, Mora D, Pfannkuchen M, Proft S, Ramon M, Vasselon V, Zimmermann J, Kahlert M (2020) Diatom DNA metabarcoding for ecological assessment: Comparison among bioinformatics pipelines used in six European countries reveals the need for standardization. Science of The Total Environment 745: 140948. https://doi.org/10.1016/j.scitotenv.2020.140948" }, { "reference": "Bey MY, Ector L (2013) Atlas des diatom\u00e9es des cours d'eau de la r\u00e9gion Rh\u00f4ne-Alpes. Direction regionale de l'Environnement, de l'Amenagement et du Logement Rhone-Alpes, Lyon, 1182 pp." }, { "reference": "BGBM (2016+) [continuously updated] Micro Algae Collection Database at B. https://diatoms.bo.berlin/algae_bgbm/sptool/sptool.php [accessed 3rd of December 2025]" }, { "reference": "Birk S, Bonne W, Borja A, Brucet S, Courrat A, Poikane S, Solimini A, van de Bund W, Zampoukas N, Hering D (2012) Three hundred ways to assess Europe's surface waters: An almost complete overview of biological methods to implement the Water Framework Directive. Ecological Indicators 18: 31\u201341. https://doi.org/10.1016/j.ecolind.2011.10.009" }, { "reference": "Blanco S, \u00c1lvarez-blanco I, Cejudo-Figueiras C, B\u00e9cares E (2012) Contribution to the diatom flora of Cambodia: Five new recent freshwater taxa. Journal of Systematics and Evolution 50: 258\u2013266. https://doi.org/10.1111/j.1759-6831.2012.00189.x" }, { "reference": "Cantonati M, Angeli N, Lange-Bertalot H (2019) Three new Fragilaria species (Bacillariophyta) from low-conductivity mountain freshwaters (Alps and Apennines). Phytotaxa 404: 261\u2013274. https://doi.org/10.11646/phytotaxa.404.6.5" }, { "reference": "CEN (2014) EN 14407:2014 Water quality \u2013 Guidance for the identification and enumeration of benthic diatom samples from rivers and lakes. Comit\u00e9 Europ\u00e9en de Normalisation, Geneva, Switzerland, 13 pp." }, { "reference": "CEN (2025) EN 13946:2025 Water quality \u2013 Guidance standard for the routine sampling and preparation of benthic diatoms from rivers and lakes. Comit\u00e9 Europ\u00e9en de Normalisation, Geneva, Switzerland, 13 pp." }, { "reference": "Chwali\u0144ska M, Karlicki M, Romac S, Not F, Karnkowska A (2025) From short to long reads: enhanced protist diversity profiling via Nanopore metabarcoding. Metabarcoding and Metagenomics 9. https://doi.org/10.3897/mbmg.9.163750" }, { "reference": "Dani M, Beszteri S, Castellanos AB, Schimani K, Skibbe O, Zimmermann J, Soares AR, Griesdorn L, Probst AJ, Kahlert M, Beszteri B (2025) Species delimitation within the Achnanthidium minutissimum complex (Bacillariophyta), based on morphological, molecular and ecophysiological approaches. Journal of Phycology 62: 25\u201343. https://doi.org/10.1111/jpy.70124" }, { "reference": "Edgar RC (2010) Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26: 2460\u20132461. https://doi.org/10.1093/bioinformatics/btq461" }, { "reference": "Epoch AI (2024) with major processing by Our World in Data. \"Number of entries in biological sequence databases\" [dataset]. Epoch AI, \"Trends in Biological Sequence Data\" [original data]. [Retrieved March 19, 2026 from] https://archive.ourworldindata.org/20260304-094028/grapher/number-of-entries-in-biological-sequence-databases.html [archived on March 4, 2026]" }, { "reference": "European Parliament and Council of the European Union (2000) Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy. Official Journal of the European Union L327, 73 pp." }, { "reference": "Gelis MMN, Barry-Martinet R, Briand J-F, Chonova T, Cochero J, Gassiole G, Kahlert M, Karthick B, Keck F, Kelly M, Kochoska H, Mann D, Nayak P, Pfannkuchen M, Servulo T, Trobajo R, Vasselon V, Vidakovic D, Viollaz L, Wetzel C, Zimmermann J, Rimet F (2025) Assigning taxonomy and traits to DNA sequences of river diatoms in a region with limited taxonomic knowledge using the updated and annotated reference library Diat.barcode. International Journal of Limnology 61: 10. https://doi.org/10.1051/limn/2025009" }, { "reference": "GGBN (2011+) [continuously updated] The GGBN Data Portal. https://www.ggbn.org [accessed 26th of July 2025]" }, { "reference": "Gon\u00e7alves S, Kahlert M, Almeida SFP, Figueira E (2018) Assessing Cu impacts on freshwater diatoms: biochemical and metabolomic responses of Tabellaria flocculosa (Roth) K\u00fctzing. Science of The Total Environment 625: 1234\u20131246. https://doi.org/10.1016/j.scitotenv.2017.12.320" }, { "reference": "Groom Q, Hyam R, G\u00fcntsch A (2017) Stable identifiers for collection specimens. Nature 546: 33\u201333. https://doi.org/10.1038/546033d" }, { "reference": "Gueidan C, Li L (2022) A long-read amplicon approach to scaling up the metabarcoding of lichen herbarium specimens. MycoKeys 86: 195\u2013212. https://doi.org/10.3897/mycokeys.86.77431" }, { "reference": "Guillard RRL, Lorenzen CJ (1972) Yellow-green algae with chlorophyllide C1,2. Journal of Phycology 8: 10\u201314. https://doi.org/10.1111/j.1529-8817.1972.tb03995.x" }, { "reference": "Hamilton PB, Lefebvre K, Bull R (2015) Single cell PCR amplification of diatoms using fresh and preserved samples. Frontiers in Microbiology 6: 16. https://doi.org/10.3389/fmicb.2015.01084" }, { "reference": "Hering D, Borja A, Jones JI, Pont D, Boets P, Bouchez A, Bruce K, Drakare S, H\u00e4nfling B, Kahlert M, Leese F, Meissner K, Mergen P, Reyjol Y, Segurado P, Vogler A, Kelly M (2018) Implementation options for DNA-based identification into ecological status assessment under the European Water Framework Directive. Water Research 138: 192\u2013205. https://doi.org/10.1016/j.watres.2018.03.003" }, { "reference": "Heudre D, Wetzel EC, Lange-Bertalot H, Van de Vijver B, Moreau L, Ector L (2021) A review of Tabellaria species from freshwater environments in Europe. Fottea 21: 180\u2013205. https://doi.org/10.5507/fot.2021.005" }, { "reference": "Hofmann G, Werum M, Lange-Bertalot H (2011) Diatomeen im S\u00fcsswasser-Benthos von Mitteleuropa. Bestimmungsflora Kieselalgen f\u00fcr die \u00f6kologische Praxis. \u00dcber 700 der h\u00e4ufigsten Arten und ihre \u00d6kologie. A.R.G.Gantner Verlag K.G., Rugell, 908 pp." }, { "reference": "Jahn R, Kusber W-H, Skibbe O, Zimmermann J, Van AT, Buczk\u00f3 K, Abarca N (2019) Gomphonella olivacea (Bacillariophyceae) \u2013 a new phylogenetic position for a well-known taxon, its typification, new species and combinations. Plant Ecology and Evolution 152(2): 219\u2013247. https://doi.org/10.5091/plecevo.2019.1603" }, { "reference": "Jahn R, Abarca N, Kusber W-H, Skibbe O, Zimmermann J, Mora D (2020) Integrative taxonomic description of two new species of the Cocconeis placentula group (Bacillariophyceae) from Korea based on unialgal strains. Algae 35: 303\u2013324. https://doi.org/10.4490/algae.2020.35.8.1" }, { "reference": "Johnson RK, Wiederholm T, Rosenberg DM (1993) Freshwater biomonitoring using individuals organisms, populations, and species assemblages of benthic macroinvertebrates. In: Rosenberg DM, Resh VH (Eds) Freshwater Biomonitoring and Benthic Invertebrates. Chapman & Hall, New York, USA, 40\u2013158. https://doi.org/10.5860/choice.30-6159" }, { "reference": "Jupke JF, Birk S, Apostolou A, Aroviita J, Baattrup-Pedersen A, Bal\u00e1\u017ei P, Bare\u0161ov\u00e1 L, Blanco S, Borrego-Ramos M, van Dam H, Dimitriou E, Feld CK, Ferreira MT, Gecheva G, Gom\u00e0 J, Han\u017eek N, Haslev IM, Isheva T, Jamoneau A, Jyrk\u00e4nkallio-Mikkola J, Kahlert M, Karaouzas I, Karjalainen SM, Olenici A, Panek P, Paril P, Peeters ETHM, Pol\u00e1\u0161ek M, Pont D, Pumputyte A, Sandin L, Sochuliakov\u00e1 L, Soininen J, Stankovi\u0107 I, Straka M, \u0160u\u0161njara M, Sutela T, Tison-Rosebery J, Udovi\u010d MG, Verhofstad M, \u017dutini\u0107 P, Sch\u00e4fer RB (2023) European river typologies fail to capture diatom, fish, and macrophyte community composition. Science of The Total Environment 896: 165081. https://doi.org/10.1016/j.scitotenv.2023.165081" }, { "reference": "Kahlert M, Albert R-L, Anttila E-L, Bengtsson R, Bigler C, Eskola T, Galman V, Gottschalk S, Herlitz E, Jarlman A, Kasperoviciene J, Kokocinski M, Luup H, Miettinen J, Paunksnyte I, Piirsoo K, Quintana I, Raunio J, Sandell B, Simola H, Sundberg I, Vilbaste S, Weckstrom J (2009) Harmonization is more important than experience-results of the first Nordic-Baltic diatom intercalibration exercise 2007 (stream monitoring). Journal of Applied Phycology 21: 471\u2013482. https://doi.org/10.1007/s10811-008-9394-5" }, { "reference": "Kahlert M, Jarlman A, Sundberg I, Herlitz E (2018) Taxalista \u2013 kiselalger i svenska s\u00f6tvatten. https://www.slu.se/contentassets/14e5c0b0cd354b7a9cbcd85181d44670/kiselalger_mall_20180405.xlsx [accessed 6th of November 2025]" }, { "reference": "Kahlert M, Kelly MG, Mann DG, Rimet F, Sato S, Bouchez A, Keck F (2019) Connecting the morphological and molecular species concepts to facilitate species identification within the genus Fragilaria (Bacillariophyta). Journal of Phycology 55: 948\u2013970. https://doi.org/10.1111/jpy.12886" }, { "reference": "Kahlert M, Aarnio S, Anijalg U, G\u00e4lman V, Karjalainen SM, Pajunen V, Teittinen A, Verweij G, Vilmi A (2021a) 6th Nordic-Baltic diatom intercalibration/harmonization exercise 2020. Diatom exercise in times of pandemics. Rapport / Sveriges lantbruksuniversitet, Institutionen f\u00f6r vatten och milj\u00f6. Institutionen f\u00f6r vatten och milj\u00f6, Uppsala, 6 pp." }, { "reference": "Kahlert M, Bailet B, Chonova T, Karjalainen SM, Schneider SC, Tapolczai K (2021b) Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes \u2013 barcodes, traits and microscope data compared. Ecological Indicators 130: 108088. https://doi.org/10.1016/j.ecolind.2021.108088" }, { "reference": "Kahlert M, R\u00fchland KM, Lavoie I, Keck F, Saulnier-Talbot E, Bogan D, Brua RB, Campeau S, Christoffersen KS, Culp JM, Karjalainen SM, Lento J, Schneider SC, Shaftel R, Smol JP (2022) Biodiversity patterns of Arctic diatom assemblages in lakes and streams: Current reference conditions and historical context for biomonitoring. Freshwater Biology 67: 116\u2013140. https://doi.org/10.1111/fwb.13490" }, { "reference": "Keck F, Vasselon V, Tapolczai K, Rimet F, Bouchez A (2017) Freshwater biomonitoring in the Information Age. Frontiers in Ecology and the Environment 15: 266\u2013274. https://doi.org/10.1002/fee.1490" }, { "reference": "Kelly M, Boonham N, Juggins S, Kille P, Mann D, Pass D, Sapp M, Sato S, Glover R (2018) A DNA based diatom metabarcoding approach for Water Framework Directive classification of rivers. Bristol, 157 pp." }, { "reference": "Kelly MG, Juggins S, Mann DG, Sato S, Glover R, Boonham N, Sapp M, Lewis E, Hany U, Kille P, Jones T, Walsh K (2020) Development of a novel metric for evaluating diatom assemblages in rivers using DNA metabarcoding. Ecological Indicators 118: 106725. https://doi.org/10.1016/j.ecolind.2020.106725" }, { "reference": "Kelly MG, Mann DG, Taylor JD, Juggins S, Walsh K, Pitt J-A, Read DS (2024) Maximising environmental pressure-response relationship signals from diatom-based metabarcoding in rivers. Science of The Total Environment 914: 169445. https://doi.org/10.1016/j.scitotenv.2023.169445" }, { "reference": "Koll\u00e1r J, Pinseel E, Vanormelingen P, Poul\u00edckov\u00e1 A, Souffreau C, Dvor\u00e1k P, Vyverman W (2019) A polyphasic approach to the delimitation of diatom species: a case study for the genus Pinnularia (Bacillariophyta). Journal of Phycology 55: 365\u2013379. https://doi.org/10.1111/jpy.12825" }, { "reference": "Koll\u00e1r J, Pinseel E, Vyverman W, Poul\u00ed\u010dkov\u00e1 A (2021) A time-calibrated multi-gene phylogeny provides insights into the evolution, taxonomy and DNA barcoding of the Pinnularia gibba group (Bacillariophyta). Fottea 21: 62\u201372. https://doi.org/10.5507/fot.2020.017" }, { "reference": "Koll\u00e1r J, Kopalov\u00e1 K, Kohler JT (2025) Diatom studies three decades into the molecular age: a bibliometric analysis reveals genetic underexploration of diatoms compared to other taxa. Fottea 25: 1\u201311. https://doi.org/10.5507/fot.2024.009" }, { "reference": "Koll\u00e1r J, Van de Vijver B, Kulichov\u00e1 J, Hejdukov\u00e1 E, Viso R, Kohler TJ, Kahlert M, Pinseel E, Poul\u00ed\u010dkov\u00e1 A, Zimmermann J, Neustupa J, Vyverman W, Kopalov\u00e1 K (2026) A polyphasic taxonomy of the pseudocryptic diatom species complex Pinnularia acidicola, including description of new species. European Journal of Phycology, 1\u201325. https://doi.org/10.1080/09670262.2026.2626682" }, { "reference": "Krammer K (2000) Diatoms of the European Inland Waters and Comparable Habitats. Vol. 1. The genus Pinnularia. A.R.G. Gantner Verlag K.G, Ruggell, 703 pp." }, { "reference": "Krammer K, Lange-Bertalot H (2004) Bacillariophyceae. 3. Teil: Centrales, Fragilariaceae, Eunotiaceae. Rev. ed. 2004. With new supplement (= p. 580\u2013599). Reprint 2008. Vol. 2/3, Gustav Fischer Verlag, Stuttgart New York, 599 pp." }, { "reference": "Kulichov\u00e1 J, Fialov\u00e1 M (2016) Correspondence between morphology and ecology: morphological variation of the Frustulia crassinervia-saxonica species complex (Bacillariophyta) reflects the ombro-minerotrophic gradient. Cryptogamie, Algologie 37: 15\u201328. https://doi.org/10.7872/crya/v37.iss1.2016.15" }, { "reference": "Kulikovskiy MS, Kociolek JP, Solak CN, Kuznetsova IV, Glushchenko AM (2023) New and interesting taxa from the diatom genus Gomphonema Ehrenberg in shallow, nearshore sites on the eastern coast of Lake Baikal. Plants 12: 1835. https://doi.org/10.3390/plants12091835" }, { "reference": "Lange-Bertalot H (2001) Diatoms of the European Inland Waters and Comparable Habitats. Vol. 2. Navicula sensu stricto. 10 Genera Separated from Navicula sensu lato. Frustulia. A.R.G. Gantner Verlag K.G, Ruggell, 526 pp." }, { "reference": "Lange-Bertalot H, Metzeltin D (1996) Indicators of Oligotrophy. Vol. 2, Koeltz Scientific Books, 390 pp." }, { "reference": "Lange-Bertalot H, Ulrich S (2014) Contributions to the taxonomy of needle-shaped Fragilaria and Ulnaria species. Lauterbornia 78: 1\u201373." }, { "reference": "Lange-Bertalot H, B\u0105k M, Witkowski A, Tagliaventi N (2011) Diatoms of the European Inland Waters and Comparable Habitats. Vol. 6. Eunotia and some related genera. A.R.G. Gantner Verlag K.G, Ruggell, 747 pp." }, { "reference": "Lindegarth M, Carstensen J, Drakare S, Johnson R, Nystr\u00f6m Sandman A, S\u00f6derpalm A, Wikstr\u00f6m SA (2016) Ecological Assessment of Swedish Water Bodies; development, harmonisation and integration of biological indicators. Final report of the research programme WATERS. Deliverable 1.1-4, WATERS report no 2016:10. Havsmilj\u00f6institutet, Sweden, 169 pp." }, { "reference": "Lindstr\u00f6m K (1991) Nutrient requirements of the dinoflagellate Peridinium gatunense. Journal of Phycology 27: 207\u2013219. https://doi.org/10.1111/j.0022-3646.1991.00207.x" }, { "reference": "Mann DG, Vanormelingen P (2013) An inordinate fondness? The number, distributions, and origins of diatom species. Journal of Eukaryotic Microbiology 60: 414\u2013420. https://doi.org/10.1111/jeu.12047" }, { "reference": "Mann DG, Sato S, Trobajo R, Vanormelingen P, Souffreau C (2010) DNA barcoding for species identification and discovery in diatoms. Cryptogamie Algologie 31: 557\u2013577." }, { "reference": "Masouras A, Karaouzas I, Dimitriou E, Tsirtsis G, Smeti E (2021) Benthic diatoms in river biomonitoring\u2014present and future perspectives within the Water Framework Directive. Water 13(4): 478. https://doi.org/10.3390/w13040478" }, { "reference": "Milj\u00f6data-MVM (2025) Swedish University of Agricultural Sciences (SLU), National data host lakes and watercourses, and national data host agricultural land. http://miljodata.slu.se/mvm/ [accessed 22th of September 2025]" }, { "reference": "Mohamad H, Mora D, Skibbe O, Abarca N, Deutschmeyer V, Enke N, Kusber W-H, Zimmermann J, Jahn R (2022) Morphological variability and genetic marker stability of 16 monoclonal pennate diatom strains under medium-term culture. Diatom Research 37: 307\u2013328. https://doi.org/10.1080/0269249X.2022.2141346" }, { "reference": "Mora D, Abarca N, Proft S, Grau JH, Enke N, Carmona J, Skibbe O, Jahn R, Zimmermann J (2019) Morphology and metabarcoding: a test with stream diatoms from Mexico highlights the complementarity of identification methods. Freshwater Science 38: 448\u2013464. https://doi.org/10.1086/704827" }, { "reference": "Morales EA, Siver PA, Trainor FR (2001) Identification of diatoms (Bacillariophyceae) during ecological assessments: comparison between light microscopy and scanning electron microscopy techniques. Proceedings of the Academy of Natural Sciences of Philadelphia 151: 95\u2013103, 109. https://doi.org/10.1635/0097-3157(2001)151[0095:IODBDE]2.0.CO;2" }, { "reference": "M\u00fcller A, G\u00fcntsch A, Jahn R, Kohlbecker A, Kusber W-H, Zimmermann J (2022) PhycoBank: Repository for algal novelties. Biodiversity Information Science and Standards 6: e90885. https://doi.org/10.3897/biss.6.90885" }, { "reference": "Pawlowski J, Kelly-Quinn M, Altermatt F, Apotheloz-Perret-Gentil L, Beja P, Boggero A, Borja A, Bouchez A, Cordier T, Domaizon I, Feio MJ, Filipe AF, Fornaroli R, Graf W, Herder J, van der Hoorn B, Iwan Jones J, Sagova-Mareckova M, Moritz C, Barquin J, Piggott JJ, Pinna M, Rimet F, Rinkevich B, Sousa-Santos C, Specchia V, Trobajo R, Vasselon V, Vitecek S, Zimmerman J, Weigand A, Leese F, Kahlert M (2018) The future of biotic indices in the ecogenomic era: Integrating (e)DNA metabarcoding in biological assessment of aquatic ecosystems. Science of The Total Environment 637\u2013638: 1295\u20131310. https://doi.org/10.1016/j.scitotenv.2018.05.002" }, { "reference": "P\u00e9rez-Burillo J, Mann DG, Trobajo R (2022) Evaluation of two short overlapping rbcL markers for diatom metabarcoding of environmental samples: Effects on biomonitoring assessment and species resolution. Chemosphere 307: 135933. https://doi.org/10.1016/j.chemosphere.2022.135933" }, { "reference": "P\u00e9rez-Burillo J, Trobajo R, Leira M, Keck F, Rimet F, Sigr\u00f3 J, Mann DG (2021) DNA metabarcoding reveals differences in distribution patterns and ecological preferences among genetic variants within some key freshwater diatom species. Science of The Total Environment 798: 149029. https://doi.org/10.1016/j.scitotenv.2021.149029" }, { "reference": "Pinseel E, Kulichov\u00e1 J, Scharfen V, Urb\u00e1nkov\u00e1 P, Van de Vijver B, Vyverman W (2019) Extensive cryptic diversity in the terrestrial diatom Pinnularia borealis (Bacillariophyceae). Protist 170: 121\u2013140. https://doi.org/10.1016/j.protis.2018.10.001" }, { "reference": "Pinseel E, Janssens SB, Verleyen E, Vanormelingen P, Kohler TJ, Biersma EM, Sabbe K, Van de Vijver B, Vyverman W (2020) Global radiation in a rare biosphere soil diatom. Nature Communications 11: 2382. https://doi.org/10.1038/s41467-020-16181-0" }, { "reference": "Reichardt E (1999) Zur Revision der Gattung Gomphonema: Die Arten um G. affine/insigne, G. angustatum/micropus, G. acuminatum sowie gomphonemoide Diatomeen aus dem Oberoligoz\u00e4n in B\u00f6hmen. Vol. 8, Koeltz Scientific Books." }, { "reference": "Rimet F (2021) A ready-to-use database for DADA2: Diat.barcode_rbcL_263bp_DADA2 based on Diat.barcode v10. https://doi.org/10.15454/D7FO2T" }, { "reference": "Rimet F, Abarca N, Bouchez A, Kusber W-H, Jahn R, Kahlert M, Keck F, Kelly M, Mann D, Piuz A, Trobajo R, Tapolczai K, Vasselon V, Zimmermann J (2018) The potential of High-Throughput Sequencing (HTS) of natural samples as a source of primary taxonomic information for reference libraries of diatom barcodes. Fottea 18: 37\u201354. https://doi.org/10.5507/fot.2017.013" }, { "reference": "Rimet F, Gusev E, Kahlert M, Kelly MG, Kulikovskiy M, Maltsev Y, Mann DG, Pfannkuchen M, Trobajo R, Vasselon V, Zimmermann J, Bouchez A (2019) Diat.barcode, an open-access curated barcode library for diatoms. Scientific Reports 9: 15116. https://doi.org/10.1038/s41598-019-51500-6" }, { "reference": "Rimet F, Aylagas E, Borja A, Bouchez A, Canino A, Chauvin C, Chonova T, Ciampor Jr F, Costa FO, Ferrari BJD, Gastineau R, Goulon C, Gugger M, Holzmann M, Jahn R, Kahlert M, Kusber W-H, Laplace-Treyture C, Leese F, Leliaert F, Mann DG, Marchand F, M\u00e9l\u00e9der V, Pawlowski J, Rasconi S, Rivera S, Rougerie R, Schweizer M, Trobajo R, Vasselon V, Vivien R, Weigand A, Witkowski A, Zimmermann J, Ekrem T (2021) Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life. Metabarcoding and Metagenomics 5: e58056. https://doi.org/10.3897/mbmg.5.58056" }, { "reference": "Rose DT, Cox EJ (2014) What constitutes Gomphonema parvulum? Long-term culture studies show that some varieties of G. parvulum belong with other Gomphonema species. Plant Ecology and Evolution 147: 366\u2013373. https://doi.org/10.5091/plecevo.2014.1059" }, { "reference": "Schimani K, Abarca N, Skibbe O, Mohamad H, Jahn R, Kusber W-H, Campana GL, Zimmermann J (2023) Exploring benthic diatom diversity in the West Antarctic Peninsula: insights from a morphological and molecular approach. Metabarcoding and Metagenomics 7: e110194. https://doi.org/10.3897/mbmg.7.110194" }, { "reference": "Simons AL, Theroux S, Osborne M, Nuzhdin S, Mazor R, Steele J (2023) Zeta diversity patterns in metabarcoded lotic algal assemblages as a tool for bioassessment. Ecological Applications 33(3): e2812. https://doi.org/10.1002/eap.2812" }, { "reference": "Smol JP, Stoermer EF (2010) The Diatoms: Applications for the Environmental and Earth Sciences. Cambridge University Press, [xviii +] 667 pp. https://doi.org/10.1017/CBO9780511763175" }, { "reference": "Solak CN, Gastineau R, Lemieux C, Turmel M, Gorecka E, Trobajo R, Rybak M, Y\u0131lmaz E, Witkowski A (2021) Nitzschia anatoliensis sp. nov., a cryptic diatom species from the highly alkaline Van Lake (Turkey). PeerJ 9: e12220. https://doi.org/10.7717/peerj.12220" }, { "reference": "Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30: 1312\u20131313. https://doi.org/10.1093/bioinformatics/btu033" }, { "reference": "Stosch HAV, Fecher K (1979) Internal thecae of Eunotia soleirolii (Bacillariophyceae) - development, structure and function as resting spores. Journal of Phycology 15: 233\u2013243. https://doi.org/10.1111/j.1529-8817.1979.tb02631.x" }, { "reference": "Strayer DL, Dudgeon D (2010) Freshwater biodiversity conservation: recent progress and future challenges. Journal of the North American Benthological Society 29: 344\u2013358. https://doi.org/10.1899/08-171.1" }, { "reference": "Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution 30: 2725\u20132729. https://doi.org/10.1093/molbev/mst197" }, { "reference": "Tapolczai K, Selmeczy GB, Szab\u00f3 B, B-B\u00e9res V, Keck F, Bouchez A, Rimet F, Padis\u00e1k J (2021) The potential of exact sequence variants (ESVs) to interpret and assess the impact of agricultural pressure on stream diatom assemblages revealed by DNA metabarcoding. Ecological Indicators 122: 107322. https://doi.org/10.1016/j.ecolind.2020.107322" }, { "reference": "The Belgian Coordinated Collections of Microorganisms (BCCM) consortium (2025) The BCCM/DCG Diatoms Collection https://bccm.belspo.be/about-DCG [accessed 6th of November 2025]" }, { "reference": "Trobajo R, Mann DG (2019) A rapid cleaning method for diatoms. Diatom Research 34: 115\u2013124. https://doi.org/10.1080/0269249X.2019.1637785" }, { "reference": "Turland N, Wiersema J, Barrie F, Gandhi K, Gravendyck J, Greuter W, Hawksworth D, Herendeen P, Klopper R, Knapp S, Kusber W-H, Li D-Z, May T, Monro A, Prado J, Price M, Smith G, Zamora JC (2025) International Code of Nomenclature for algae, fungi, and plants (Madrid Code). University of Chicago Press. https://doi.org/10.7208/chicago/9780226839479.001.0001" }, { "reference": "Urbankova P, Vesela J (2013) DNA-barcoding: A case study in the diatom genus Frustulia (Bacillariophyceae). Nova Hedwigia: 147\u2013162." }, { "reference": "Urb\u00e1nkov\u00e1 P, Scharfen V, Kulichov\u00e1 J (2016) Molecular and automated identification of the diatom genus Frustulia in northern Europe. Diatom Research 31: 217\u2013229. https://doi.org/10.1080/0269249x.2016.1224780" }, { "reference": "Van de Vijver B, Williams DM (2022) Four new Fragilaria (Fragilariaceae, Bacillariophyta) species in Europe with coarse striae. Nova Hedwigia 115: 317\u2013347. https://doi.org/10.1127/nova_hedwigia/2022/0722" }, { "reference": "Van de Vijver B, Schuster TM, Kusber W-H, Hamilton PB, Wetzel CE, Ector L (2021a) Revision of European Brachysira species (Brachysiraceae, Bacillariophyta): I. The Brachysira microcephala - B. neoexilis enigma. Botany Letters 168: 467\u2013484. https://doi.org/10.1080/23818107.2021.1909499" }, { "reference": "Van de Vijver B, Williams MD, Kelly M, Jarlman A, Wetzel EC, Ector L (2021b) Analysis of some species resembling Fragilaria capucina (Fragilariaceae, Bacillariophyta). Fottea 21: 128\u2013151. https://doi.org/10.5507/fot.2021.001" }, { "reference": "Van de Vijver B, Schuster TM, Kusber W-H, Kennedy B, Hamilton PB, Albert R-L, Ballings P, Wetzel CE, Ector L (2022a) Revision of European Brachysira species (Brachysiraceae, Bacillariophyta): III. Species formerly included in the Brachysira serians-complex. Botany Letters 169: 83\u2013105. https://doi.org/10.1080/23818107.2021.1941250" }, { "reference": "Van de Vijver B, Williams D, Kusber W-H, Cantonati M, Hamilton P, Wetzel C, Ector L (2022b) Fragilaria radians (K\u00fctzing) D.M.Williams et Round, the correct name for F. gracilis (Fragilariaceae, Bacillariophyta): a critical analysis of this species complex in Europe. Fottea 22: 256\u2013291. https://doi.org/10.5507/fot.2022.006" }, { "reference": "Van de Vijver B, Williams D, Schuster T, Kusber W-H, Cantonati M, Wetzel C, Ector L (2022c) Analysis of the Fragilaria rumpens complex (Fragilariaceae, Bacillariophyta) with the description of two new species. Journal of the Czech Phycological Society 22: 93\u2013121. https://doi.org/10.5507/fot.2021.018" }, { "reference": "Van de Vijver B, Ector L, Wetzel C (2023a) Taxonomic diatom workshop Planothidium. https://www.nvkd-diatom.nl/_files/ugd/f21ff7_84c46f3e76c24398aff448f25912d98b.pdf [accessed 8th of August 2023]" }, { "reference": "Van de Vijver B, Schuster MT, J\u00f3nsson SG, Hansen I, Williams MD, Kusber W-H, Wetzel EC, Ector L (2023b) A critical analysis of the Fragilaria vaucheriae complex (Bacillariophyta) in Europe. Fottea 23: 62\u201396. https://doi.org/10.5507/fot.2022.013" }, { "reference": "Vanormelingen P, Chepurnov VA, Mann DG, Sabbe K, Vyverman W (2008) Genetic divergence and reproductive barriers among morphologically heterogeneous sympatric clones of Eunotia bilunaris sensu lato (Bacillariophyta). Protist 159: 73\u201390. https://doi.org/10.1016/j.protis.2007.08.004" }, { "reference": "Vaulot D, Bilcke G, Chaerle P, Falciatore A, Gourvil P, Lomas MW, Probert I, Vyverman W (2023) Culturing Diatoms. In: Goessling JW, Ser\u00f4dio J, Lavaud J (Eds) Diatom Photosynthesis: From Primary Production to High Value Molecules. Wiley-Scrivener. https://doi.org/10.1002/9781119842156.ch14" }, { "reference": "Weigand H, Beermann AJ, \u010ciampor F, Costa FO, Csabai Z, Duarte S, Geiger MF, Grabowski M, Rimet F, Rulik B, Strand M, Szucsich N, Weigand AM, Willassen E, Wyler SA, Bouchez A, Borja A, \u010ciamporov\u00e1-Za\u0165ovi\u010dov\u00e1 Z, Ferreira S, Dijkstra K-DB, Eisendle U, Freyhof J, Gadawski P, Graf W, Haegerbaeumer A, van der Hoorn BB, Japoshvili B, Keresztes L, Keskin E, Leese F, Macher JN, Mamos T, Paz G, Pe\u0161i\u0107 V, Pfannkuchen DM, Pfannkuchen MA, Price BW, Rinkevich B, Teixeira MAL, V\u00e1rb\u00edr\u00f3 G, Ekrem T (2019) DNA barcode reference libraries for the monitoring of aquatic biota in Europe: Gap-analysis and recommendations for future work. Science of The Total Environment 678: 499\u2013524. https://doi.org/10.1016/j.scitotenv.2019.04.247" }, { "reference": "Zimmermann J, Jahn R, Gemeinholzer B (2011) Barcoding diatoms: evaluation of the V4 subregion on the 18S rRNA gene, including new primers and protocols. Organisms Diversity & Evolution 11: 173\u2013192. https://doi.org/10.1007/s13127-011-0050-6" }, { "reference": "Zimmermann J, Abarca N, Enk N, Skibbe O, Kusber W-H, Jahn R (2014) Taxonomic reference libraries for environmental barcoding: A best practice example from diatom research. PLOS ONE 9: e108793. https://doi.org/10.1371/journal.pone.0108793" }, { "reference": "Zou S, Bao Y, Wu X, Wang C (2021) DNA barcoding diatoms from China with multiple genes. Frontiers in Marine Science 8: 698331. https://doi.org/10.3389/fmars.2021.698331" } ], "related_identifiers": [ { "identifier": "10.3897/mbmg.10.186778.suppl1", "relation_type": { "id": "haspart", "title": { "de": "Umfasst folgenden Teil", "en": "Has part" } }, "resource_type": { "id": "other", "title": { "de": "Sonstige", "en": "Other" } }, "scheme": "doi" }, { "identifier": "10.3897/mbmg.10.186778.suppl2", "relation_type": { "id": "haspart", "title": { "de": "Umfasst folgenden Teil", "en": "Has part" } }, "resource_type": { "id": "other", "title": { "de": "Sonstige", "en": "Other" } }, "scheme": "doi" }, { "identifier": "10.3897/mbmg.10.186778.suppl3", "relation_type": { "id": "haspart", "title": { "de": "Umfasst folgenden Teil", "en": "Has part" } }, "resource_type": { "id": "other", "title": { "de": "Sonstige", "en": "Other" } }, "scheme": "doi" }, { "identifier": "10.1371/journal.pone.0086885", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1080/23818107.2019.1694980", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/fwb.14360", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/mec.15646", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.3897/mbmg.3.34002", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.scitotenv.2020.140948", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.ecolind.2011.10.009", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/j.1759-6831.2012.00189.x", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.11646/phytotaxa.404.6.5", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.3897/mbmg.9.163750", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/jpy.70124", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1093/bioinformatics/btq461", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1051/limn/2025009", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.scitotenv.2017.12.320", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1038/546033d", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.3897/mycokeys.86.77431", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/j.1529-8817.1972.tb03995.x", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.3389/fmicb.2015.01084", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.watres.2018.03.003", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5507/fot.2021.005", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5091/plecevo.2019.1603", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.4490/algae.2020.35.8.1", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5860/choice.30-6159", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.scitotenv.2023.165081", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1007/s10811-008-9394-5", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/jpy.12886", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.ecolind.2021.108088", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/fwb.13490", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1002/fee.1490", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.ecolind.2020.106725", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.scitotenv.2023.169445", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/jpy.12825", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5507/fot.2020.017", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5507/fot.2024.009", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1080/09670262.2026.2626682", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.7872/crya/v37.iss1.2016.15", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.3390/plants12091835", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/j.0022-3646.1991.00207.x", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/jeu.12047", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.3390/w13040478", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1080/0269249X.2022.2141346", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1086/704827", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1635/0097-3157(2001)151", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.3897/biss.6.90885", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.scitotenv.2018.05.002", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.chemosphere.2022.135933", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.scitotenv.2021.149029", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.protis.2018.10.001", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1038/s41467-020-16181-0", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.15454/D7FO2T", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5507/fot.2017.013", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1038/s41598-019-51500-6", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.3897/mbmg.5.58056", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5091/plecevo.2014.1059", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.3897/mbmg.7.110194", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1002/eap.2812", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1017/CBO9780511763175", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.7717/peerj.12220", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1093/bioinformatics/btu033", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1111/j.1529-8817.1979.tb02631.x", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1899/08-171.1", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1093/molbev/mst197", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.ecolind.2020.107322", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1080/0269249X.2019.1637785", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.7208/chicago/9780226839479.001.0001", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1080/0269249x.2016.1224780", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1127/nova_hedwigia/2022/0722", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1080/23818107.2021.1909499", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5507/fot.2021.001", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1080/23818107.2021.1941250", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5507/fot.2022.006", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5507/fot.2021.018", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.5507/fot.2022.013", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.protis.2007.08.004", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1002/9781119842156.ch14", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1016/j.scitotenv.2019.04.247", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1007/s13127-011-0050-6", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.1371/journal.pone.0108793", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" }, { "identifier": "10.3389/fmars.2021.698331", "relation_type": { "id": "cites", "title": { "de": "Zitiert", "en": "Cites" } }, "resource_type": { "id": "publication", "title": { "de": "Publikation", "en": "Publication" } }, "scheme": "doi" } ], "resource_type": { "id": "publication-article", "title": { "de": "Zeitschriftenartikel", "en": "Journal article" } }, "rights": [ { "description": { "en": "The Creative Commons Attribution license allows re-distribution and re-use of a licensed work on the condition that the creator is appropriately credited." }, "icon": "cc-by-icon", "id": "cc-by-4.0", "props": { "scheme": "spdx", "url": "https://creativecommons.org/licenses/by/4.0/legalcode" }, "title": { "en": "Creative Commons Attribution 4.0 International" } } ], "subjects": [ { "subject": "18SV4" }, { "subject": "DNA library" }, { "subject": "environmental monitoring" }, { "subject": "metabarcoding" }, { "subject": "molecular sequence data" }, { "subject": "rbcL" }, { "subject": "Water Framework Directive" }, { "subject": "water quality" } ], "title": "New DNA barcode reference data of freshwater diatoms (Bacillariophyceae) from Sweden: old acquaintances and new taxa" }, "parent": { "access": { "owned_by": { "user": "22270" }, "settings": { "accept_conditions_text": null, "allow_guest_requests": false, "allow_user_requests": false, "secret_link_expiration": 0 } }, "communities": { "default": "c529f97d-f8cb-4c13-a439-9e36891694c2", "entries": [ { "access": { "member_policy": "open", "members_visibility": "public", "record_submission_policy": "open", "review_policy": "open", "visibility": "public" }, "children": { "allow": true }, "created": "2013-11-11T14:14:18+00:00", "custom_fields": {}, "deletion_status": { "is_deleted": false, "status": "P" }, "id": "c529f97d-f8cb-4c13-a439-9e36891694c2", "links": {}, "metadata": { "curation_policy": "The Biodiversity Literature Repository (BLR) is to share publications related to bio-systematics. The goal is to provide
\nFor additional search functionality refindit can be used. This includes also searches in CrossRef, DataCite, PubMed, RefBank, GNUB and Mendeley.
\nUpload guideline
\nA provisional guideline how to upload is here.
\n\n
\n
\n
\n
", "title": "Biodiversity Literature Repository", "type": { "id": "topic" }, "website": "https://www.biolitrepo.org" }, "revision_id": 15, "slug": "biosyslit", "theme": { "brand": "biosyslit", "enabled": true, "style": { "font": { "family": "Arial, sans-serif", "size": "16px", "weight": 600 }, "mainHeaderBackgroundColor": "#FFFFFF", "primaryColor": "#636363", "primaryTextColor": "#FFFFFF", "secondaryColor": "#7eb13e", "secondaryTextColor": "#FFFFFF", "tertiaryColor": "#7eb13e", "tertiaryTextColor": "#FFFFFF" } }, "updated": "2025-04-07T12:05:37.615917+00:00" } ], "ids": [ "c529f97d-f8cb-4c13-a439-9e36891694c2" ] }, "id": "20424412", "pids": {} }, "pids": { "doi": { "identifier": "10.3897/mbmg.10.186778", "provider": "external" }, "oai": { "identifier": "oai:zenodo.org:20424413", "provider": "oai" } }, "revision_id": 5, "stats": { "all_versions": { "data_volume": 17806231.0, "downloads": 14, "unique_downloads": 13, "unique_views": 11, "views": 14 }, "this_version": { "data_volume": 17806231.0, "downloads": 14, "unique_downloads": 13, "unique_views": 11, "views": 14 } }, "status": "published", "swh": {}, "updated": "2026-05-28T07:30:49.900997+00:00", "versions": { "index": 1, "is_latest": true } }