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Published August 4, 2018 | Version v1
Dataset Open

Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells – 2018/08/04

  • 1. Structural Genomics Consortium, University of Toronto

Description

Project - Huntingtin structure-function open lab notebook. 

Rationale - Purified huntingtin samples with and without stabilising binding partners (HAP40) are required for use in structural and functional studies, in particular SAXS experiments. 

Notes

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.

Files

Large scale expression and purification of full-length huntingtin Q54 with HAP40 from baculoviral expression system production in sf9 insect cells 20180804.pdf