TABLE 2. List of Hygrobates and Atractides specimens, respectively used for building the Neighbour-Joining (NJ) trees (Figures 2 and 5). Sequences marked with an asterisk were derived from the GenBank database, otherwise, sequences are downloaded from the BOLD database. BINs are based on the barcode analysis from 10 November 2025.
| Process ID |
Sample ID |
Country |
BIN |
| Hygrobates anatolicus |
| HYDOC037-22 |
CCDB 44300 D01 |
Türkiye |
BOLD:AEX2126 |
| HYDOC045-22 |
CCDB 44300 D09 |
Türkiye |
| HYDOC048-22| |
CCDB 44300 D12 |
Türkiye |
| HYDOC036-22 |
CCDB 44300 C12 |
Türkiye |
BOLD:AEY8569 |
| HYDOC044-22 |
CCDB 44300 D08 |
Türkiye |
| HYDOC046-22 |
CCDB 44300 D10 |
Türkiye |
| HYDOC047-22 |
CCDB 44300 D11 |
Türkiye |
| Hygrobates arenarius |
| NLACA240-15 |
RMNH.ACA.508 |
The Netherlands |
BOLD:AAM2654 |
| Hygrobates calliger |
| DNCBD004-20 |
CCDB-38679-A04 |
Montenegro |
BOLD:AEF4261 |
| Hygrobates crypticus |
| HYDME047-22 |
CCDB 41823 D11 |
Türkiye |
BOLD:AET8584 |
| Hygrobates fluviatilis |
| NLACA898-15 |
RMNH.ACA.1485 |
The Netherlands |
BOLD:ACB4846 |
| Hygrobates foreli |
| MARBN1230-23 |
MARB UIB 838 |
Norway |
BOLD:AAM5295 |
| Hygrobates grabowskii |
| HDRIZ021-24 |
CCDB 48502 B09 |
Türkiye |
BOLD:AFH9674 |
| Hygrobates longipalpis |
| HYDCG017-24 |
CCDB-48498-B05 |
Türkiye |
BOLD:AES0232 |
| Hygrobates longiporus |
| SEPTA047-2 |
CCDB 38363 D11 |
Serbia |
BOLD:AEM7299 |
| Hygrobates nigromaculatus |
| HYDCA082-15 |
HYDCA82 |
Norway |
BOLD:AAM1669 |
| Hygrobates ponticus yildirimi |
| HYDIR016-23 |
CCDB 39399 B04 |
Türkiye |
BOLD:AEY7516 |
| HYDIR030-23 |
CCDB 39399 C06 |
Türkiye |
| HYDOC059-22 |
CCDB 44300 E11 |
Türkiye |
| HYDOC057-22 |
CCDB 44300 E09 |
Türkiye |
| HDRIZ023-24 |
CCDB 48502 B11 |
Türkiye |
BOLD:AGO8960 |
| Hygrobates ponticus ponticus |
| NOVMD076-21 |
CCDB 41822 G04 |
Georgia |
BOLD:AEO0649 |
| NOVMD004-21 |
CCDB 41822 A04 |
Georgia |
| HYDME054-22 |
CCDB 41823 E06 |
Türkiye |
| NOVMD056-21 |
CCDB 41822 E08 |
Georgia |
| Hygrobates prosiliens |
| MMHYD097-19 |
HYDCA231 |
Norway |
BOLD:AAX2654 |
| Hygrobates setosus |
| MARBN1229-23 |
MARB UIB 837 |
Norway |
BOLD:AAM1668 |
| Hygrobates thori |
| HYDOC043-22 |
CCDB 44300 D07 |
Türkiye |
BOLD:AEL4319 |
| Hygrobates turcicus |
| HYDOC035-22 |
CCDB 44300 C11 |
Türkiye |
BOLD:ADX1364 |
| Atractides gibberipalpis |
| HYDAS158-22 |
CCDB 39398 F03 |
Austria |
HYDAS158-22 |
| HYDAS122-22 |
CCDB 39398 C03 |
Austria |
| HYDAS043-22 |
CCDB 39397 D07 |
Austria |
| DCDDJ031-21 |
CCDB 38361 C07 |
Montenegro |
BOLD:AEK7766 |
| HYDBH003-22 |
CCDB 41824 A03 |
Montenegro |
BOLD:AFI2792 |
| HYDBH004-22 |
CCDB 41824 A04 |
Montenegro |
BOLD:AEI3946 |
| DCCDB014-21 |
CCDB38233 B02 |
Montenegro |
| HYDBH001-22 |
CCDB 41824 A01 |
Montenegro |
BOLD:ADU2475 |
| LBCWS219-19 |
CrenoBarcode H508 |
Germany |
| NOVMB070-21 |
CCDB 38559 F10 |
France (Corsica) |
BOLD:AEO4226 |
| NOVMB032-21 |
CCDB 38559 C08 |
France (Corsica) |
| NOVMB082-21 |
CCDB 38559 G10 |
France (Corsica) |
| NOVMB071-21 |
CCDB 38559 F11 |
France (Corsica) |
| NOVMB044-21 |
CCDB 38559 D08 |
France (Corsica) |
| NOVMB091-21 |
CCDB 38559 H07 |
France (Corsica) |
| NOVMB010-21 |
CCDB 38559 A10 |
France (Corsica) |
| NOVMB081-21 |
CCDB 38559 G09 |
France (Corsica) |
| NOVMB019-21 |
CCDB 38559 B07 |
France (Corsica) |
| Atractides inflatus |
| NOVMB040-21 |
CCDB 38559 D04 |
France (Corsica) |
BOLD:AEO0988 |
| BSNTN1979-24 |
BGE 00247 G0 |
Greece |
BOLD:AFI9009 |
| BSNTN528-23 |
BGE 00110 E05 |
Portugal |
| HYDIR034-23 |
CCDB 39399 C10 |
Türkiye |
BOLD:ACB4677 |
| BSNTN898-23 |
BGE 00228 D07 |
Portugal |
| BBIOP043-24 |
BGE 00109 D07 |
Portugal |
| NOVMB013-21 |
CCDB 38559 B01 |
France (Corsica) |
| DNAEC052-20 |
14. M19 12 4 E5 |
Montenegro |
| HYDIR032-23 |
CCDB 39399 C08 |
Türkiye |
| HYDIR026-23 |
CCDB 39399 C02 |
Türkiye |
| SEPTB021-21 |
CCDB 38362 B09 |
Greece |
| SEPTB060-21 |
CCDB 38362 E12 |
Türkiye |
| DCDDJ061-21 |
CCDB 38361 F01 |
Türkiye |
| HYDIR033-23 |
CCDB 39399 C09 |
Türkiye |
| HYDIR031-23 |
CCDB 39399 C07 |
Türkiye |
| DCDDJ058-21 |
CCDB 38361 E10 |
Türkiye |
| HYDIR028-23 |
CCDB 39399 C04 |
Türkiye |
| DCDDJ060-21 |
CCDB 38361 E12 |
Türkiye |
| Atractides nodipalpis |
| NLACA1118-17 |
RMNH.ACA.1533 |
The Netherlands |
BOLD:ACR0209 |
| Atractides subasper |
| HYDOC007-22 |
CCDB 44300 A07 |
Serbia |
BOLD:AEX4044 |
| Atractides tener |
| MARBN1114-23 |
MARB UIB 627 |
Norway |
BOLD:ACG4776 |