TABLE 1. Details of DNA barcoded specimens, including localities and coordinates of sampling sites, and the barcode index number codes (^N indicates a new BIN that contains only sequences from this study). BOLD data presented here was last accessed on 1 ^th November 2025. The sampling sites marked with an asterisk (*) belong to the Uzungöl Lake basin, the other localities belong to the Black Sea basin.

Locality	Coordinates	Sample ID	Process ID	BIN
Sperchontidae
Sperchon amuzgari complex
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*Trabzon, Çaykara, Uzungöl Lake basin, Çataklı Stream	40.767223° N, 40.25611° E	CCDB 48502 D04	HDRIZ040-24	NBOLD:AGO5563
Trabzon, Maçka, AltıntaŞ Creek near Sumelias Camping	40.784443° N, 39.61361° E	ACR-49	WMRIZ033-25
Sperchon clupeifer
*Trabzon, Çaykara, Uzungöl Lake basin, Çataklı Stream	40.76722° N, 40.25611° E	CCDB 48502 D03	HDRIZ039-24	BOLD:AGK2889
Trabzon, KöprübaŞı, Mahanas Stream	40.76000° N, 40.11361° E	ACR-51	WMRIZ035-25
Sperchon milisai
*Trabzon, Çaykara, Uzungöl Lake basin, Çataklı Stream	40.76722° N, 40.25611° E	CCDB 48502 D05	HDRIZ041-24	BOLD:AGO5836
Trabzon, Araklı, tributary of Karadere Stream	40.86055° N, 40.03111° E	ACR-37	WMRIZ028-25
Lebertiidae
Lebertia porosa aggr.
Trabzon, Maçka, Kalyan Creek	40.86194° N, 39.69805° E	ACR-3 ACR-4	WMRIZ003-25 WMRIZ004-25	BOLD:AHE4641
Trabzon, Maçka, Hamsiköy Stream	40.68833° N, 39.45472° E	ACR-14	WMRIZ012-25
Torrenticolidae
Torrenticola eseni
Trabzon, Maçka, AltıntaŞ Creek	40.76389° N, 39.60083° E	CCDB 48502 C11	HDRIZ035-24
Trabzon, Maçka, AltıntaŞ Creek near Sümela Camping	40.78444° N, 39.61361° E	CCDB 48502 B12	HDRIZ024-24	BOLD:AGO5554
*Trabzon, Çaykara, Uzungöl Lake basin, Çataklı Stream	40.76722° N, 40.25611° E	CCDB 48502 D07	HDRIZ043-24
*Trabzon, Çaykara, Uzungöl Lake basin, Çataklı Stream	40.76722° N, 40.25611° E	ACR-28 ACR-31	WMRIZ023-25 WMRIZ025-25	BOLD:AHC5017
Trabzon, Maçka, AltıntaŞ Creek near Sümela Camping	40.78444° N, 39.61361° E	ACR-50	WMRIZ034-25
Monatractides madritensis
Trabzon, Maçka, AltıntaŞ Creek near Sümela Camping	40.78444° N, 39.61361° E	CCDB 48502 B10	HDRIZ022-24	BOLD:AGO5560
Hygrobatidae
Atractides karadenizensis sp. nov.
Rize, İkizdere, Çokcor Stream	40.68667° N, 40.70111° E	CCDB 48502 B01 CCDB 48502 B02	HDRIZ013-24 HDRIZ014-24	BOLD:AGO5566
*Trabzon, Çaykara, Uzungöl Lake basin, Çataklı Stream	40.76722° N, 40.25611° E	ACR-32	WMRIZ026-25
Atractides gibberipalpis
*Trabzon, Çaykara, stream, tributary of Uzungöl Lake	40.61694° N, 40.29250° E	CCDB 48502 D02	HDRIZ038-24	BOLD:ADU2475
Trabzon, KöprübaŞı, Mahanas Stream	40.76000° N, 40.11361° E	ACR-21	WMRIZ017-25
Atractides remotus
Trabzon, Maçka, AltıntaŞ Creek	40.76389° N, 39.60083° E	CCDB 48502 C08 CCDB 48502 C09 CCDB 48502 C10	HDRIZ032-24 HDRIZ033-24 HDRIZ034-24	BOLD:AGO5564
Trabzon, KöprübaŞı, Mahanas Stream	40.76000° N, 40.11361° E	CCDB 48502 C04	HDRIZ028-24	BOLD:AGO5564
Rize, İkizdere, BaŞköy Stream	40.78333° N, 40.57777° E	ACR-7	WMRIZ007-25
*Trabzon, Caykara, stream, tributary of Uzungöl Lake	40.61694° N, 40.29250° E	CCDB 48502 D01 ACR-17	HDRIZ037-24 WMRIZ015-25	BOLD:AHC6684
Rize, ArdeŞen, Akkaya Village, tributary of Fırtına River	41.13250° N, 41.01666° E	CCDB 48502 A12 ACR-53	HDRIZ012-24 WMRIZ036-25	BOLD:AHC6683
Hygrobates turani sp. nov.
Trabzon, Maçka, Hamsiköy Stream	40.68833° N, 39.45472° E	CCDB 48502 C01	HDRIZ025-24	BOLD:AGO8958
Hygrobates grabowskii
Trabzon, Maçka, Kalyan Creek	40.86194° N, 39.69805° E	CCDB 48502 B09 ACR-11	HDRIZ021-24 WMRIZ011-25	BOLD:AFH9674
Hygrobates longipalpis
*Trabzon, Çaykara, Uzungöl Lake	40.61778° N, 40.29639° E	CCDB 48502 B06 ACR-8 ACR-9	HDRIZ018-24 WMRIZ008-25 WMRIZ009-25	BOLD:AES0232
Trabzon, KöprübaŞı, drainage basin of Mahanas Stream, spring	40.76000° N, 40.11361° E	ACR-42	WMRIZ030-25
Hygrobates ponticus
Trabzon, Maçka, AltıntaŞ Creek near Sümela Camping	40.78444° N, 39.61361° E	CCDB 48502 B11	HDRIZ023-24	BOLD:AGO8960
Hygrobates turcicus
*Trabzon, Çaykara, Uzungöl Lake basin, Çataklı Stream	40.76722° N, 40.25611° E	ACR-30	WMRIZ024-25
Trabzon, Araklı, tributary of Karadere Stream	40.86055° N, 40.03111° E	ACR-39	WMRIZ029-25	BOLD:ADX1364
Rize, ArdeŞen, Akkaya Village, tributary of Fırtına River	41.13250° N, 41.01666° E	ACR-54	WMRIZ037-25
Mideopsidae
Mideopsis roztoczensis
Trabzon, Maçka, Kalyan Creek	40.86194° N, 39.69805° E	ACR-6	WMRIZ006-25	BOLD:AHC5495