Pipeline Report

Created by Bpipe, Sat Dec 03 12:08:01 COT 2016


Result


Succeeded

Timeline


0.304 s
0.031 s
0.030 s
28.166 s
28.166 s
0.050 s
0.044 s
0.565 s
0.565 s
0.104 s
0.054 s
0.719 s
0.719 s
0.035 s
0.048 s
1.750 s
1.750 s
0.038 s
0.057 s
1.203 s
1.203 s
0.141 s
0.156 s
0.100 s
0.101 s

Pipeline Stages


Total Runtime = 17 hours, 7 minutes, 50.519 seconds

  1. prepare_dir : Make work directories

    Creates directories for organizing files from all pipeline stages.

    Author nfranco@eafit.edu.co
    Inputs
    • 18s.fasta
    • 28s.fasta
    • 58s.fasta
    • ITS1.fasta
    • ITS2.fasta
    Commandsmkdir prot_seqs alignments alignments/prot alignments/dna partitionfinder phylo_analyses phylo_analyses/mrbayes phylo_analyses/garli phylo_analyses/raxml
    Outputs
    • 18s.fasta
    Execution Time 2016-12-2 19:0:10 (0.304 seconds)
  • check_for_cds : Check input FASTA file status

    Looks for identifiers in input filenames in order to redirect files properly according their status (coding sequence '_cds.fasta', provided alignment '_al.fasta' and noncoding sequence).

    Author nfranco@eafit.edu.co
    Inputs
    • 18s.fasta
    Commands
    Outputs
    • 18s.fasta
    Execution Time 2016-12-2 19:0:11 (0.031 seconds)
  • translate : Translate coding sequences using DNA2PEP

    Translates protein coding sequences to amino acid sequences given the appropiate genetic code table (default: 1).

    Author nfranco@eafit.edu.co
    Inputs
    • 18s.fasta
    Commands
    Outputs
    • 18s.fasta
    Execution Time 2016-12-2 19:0:11 (0.030 seconds)
  • align : Align FASTA files using MAFFT

    This stage aligns FASTA files (nucleotide or amino acid sequences) if required according input file status.

    Author nfranco@eafit.edu.co
    Inputs
    • 18s.fasta
    Commands/home/bio/phypipe/scripts/apps/mafft/bin/mafft --quiet --auto 18s.fasta > alignments/dna/18s.DNA.alignment.fasta
    Outputs
    • alignments/dna/18s.DNA.alignment.fasta
    Execution Time 2016-12-2 19:0:11 - 2016-12-2 19:0:40 (28.396 seconds)
  • reverse_translate : Convert amino acid alignment to nucleotides using RevTrans

    This stage transforms FASTA amino acid alignment file to nucleotide alignment matrix.

    Author nfranco@eafit.edu.co
    Inputs
    • alignments/dna/18s.DNA.alignment.fasta
    Commands
    Outputs
    • alignments/dna/18s.DNA.alignment.fasta
    Execution Time 2016-12-2 19:0:40 (0.006 seconds)
  • check_for_cds : Check input FASTA file status

    Looks for identifiers in input filenames in order to redirect files properly according their status (coding sequence '_cds.fasta', provided alignment '_al.fasta' and noncoding sequence).

    Author nfranco@eafit.edu.co
    Inputs
    • 28s.fasta
    Commands
    Outputs
    • 28s.fasta
    Execution Time 2016-12-2 19:0:11 (0.050 seconds)
  • translate : Translate coding sequences using DNA2PEP

    Translates protein coding sequences to amino acid sequences given the appropiate genetic code table (default: 1).

    Author nfranco@eafit.edu.co
    Inputs
    • 28s.fasta
    Commands
    Outputs
    • 28s.fasta
    Execution Time 2016-12-2 19:0:11 (0.044 seconds)
  • align : Align FASTA files using MAFFT

    This stage aligns FASTA files (nucleotide or amino acid sequences) if required according input file status.

    Author nfranco@eafit.edu.co
    Inputs
    • 28s.fasta
    Commands/home/bio/phypipe/scripts/apps/mafft/bin/mafft --quiet --auto 28s.fasta > alignments/dna/28s.DNA.alignment.fasta
    Outputs
    • alignments/dna/28s.DNA.alignment.fasta
    Execution Time 2016-12-2 19:0:11 (0.893 seconds)
  • reverse_translate : Convert amino acid alignment to nucleotides using RevTrans

    This stage transforms FASTA amino acid alignment file to nucleotide alignment matrix.

    Author nfranco@eafit.edu.co
    Inputs
    • alignments/dna/28s.DNA.alignment.fasta
    Commands
    Outputs
    • alignments/dna/28s.DNA.alignment.fasta
    Execution Time 2016-12-2 19:0:12 (0.011 seconds)
  • check_for_cds : Check input FASTA file status

    Looks for identifiers in input filenames in order to redirect files properly according their status (coding sequence '_cds.fasta', provided alignment '_al.fasta' and noncoding sequence).

    Author nfranco@eafit.edu.co
    Inputs
    • 58s.fasta
    Commands
    Outputs
    • 58s.fasta
    Execution Time 2016-12-2 19:0:11 (0.104 seconds)
  • translate : Translate coding sequences using DNA2PEP

    Translates protein coding sequences to amino acid sequences given the appropiate genetic code table (default: 1).

    Author nfranco@eafit.edu.co
    Inputs
    • 58s.fasta
    Commands
    Outputs
    • 58s.fasta
    Execution Time 2016-12-2 19:0:11 (0.054 seconds)
  • align : Align FASTA files using MAFFT

    This stage aligns FASTA files (nucleotide or amino acid sequences) if required according input file status.

    Author nfranco@eafit.edu.co
    Inputs
    • 58s.fasta
    Commands/home/bio/phypipe/scripts/apps/mafft/bin/mafft --quiet --auto 58s.fasta > alignments/dna/58s.DNA.alignment.fasta
    Outputs
    • alignments/dna/58s.DNA.alignment.fasta
    Execution Time 2016-12-2 19:0:11 (0.947 seconds)
  • reverse_translate : Convert amino acid alignment to nucleotides using RevTrans

    This stage transforms FASTA amino acid alignment file to nucleotide alignment matrix.

    Author nfranco@eafit.edu.co
    Inputs
    • alignments/dna/58s.DNA.alignment.fasta
    Commands
    Outputs
    • alignments/dna/58s.DNA.alignment.fasta
    Execution Time 2016-12-2 19:0:12 (0.013 seconds)
  • check_for_cds : Check input FASTA file status

    Looks for identifiers in input filenames in order to redirect files properly according their status (coding sequence '_cds.fasta', provided alignment '_al.fasta' and noncoding sequence).

    Author nfranco@eafit.edu.co
    Inputs
    • ITS1.fasta
    Commands
    Outputs
    • ITS1.fasta
    Execution Time 2016-12-2 19:0:11 (0.035 seconds)
  • translate : Translate coding sequences using DNA2PEP

    Translates protein coding sequences to amino acid sequences given the appropiate genetic code table (default: 1).

    Author nfranco@eafit.edu.co
    Inputs
    • ITS1.fasta
    Commands
    Outputs
    • ITS1.fasta
    Execution Time 2016-12-2 19:0:11 (0.048 seconds)
  • align : Align FASTA files using MAFFT

    This stage aligns FASTA files (nucleotide or amino acid sequences) if required according input file status.

    Author nfranco@eafit.edu.co
    Inputs
    • ITS1.fasta
    Commands/home/bio/phypipe/scripts/apps/mafft/bin/mafft --quiet --auto ITS1.fasta > alignments/dna/ITS1.DNA.alignment.fasta
    Outputs
    • alignments/dna/ITS1.DNA.alignment.fasta
    Execution Time 2016-12-2 19:0:11 - 2016-12-2 19:0:13 (1.944 seconds)
  • reverse_translate : Convert amino acid alignment to nucleotides using RevTrans

    This stage transforms FASTA amino acid alignment file to nucleotide alignment matrix.

    Author nfranco@eafit.edu.co
    Inputs
    • alignments/dna/ITS1.DNA.alignment.fasta
    Commands
    Outputs
    • alignments/dna/ITS1.DNA.alignment.fasta
    Execution Time 2016-12-2 19:0:13 (0.006 seconds)
  • check_for_cds : Check input FASTA file status

    Looks for identifiers in input filenames in order to redirect files properly according their status (coding sequence '_cds.fasta', provided alignment '_al.fasta' and noncoding sequence).

    Author nfranco@eafit.edu.co
    Inputs
    • ITS2.fasta
    Commands
    Outputs
    • ITS2.fasta
    Execution Time 2016-12-2 19:0:11 (0.038 seconds)
  • translate : Translate coding sequences using DNA2PEP

    Translates protein coding sequences to amino acid sequences given the appropiate genetic code table (default: 1).

    Author nfranco@eafit.edu.co
    Inputs
    • ITS2.fasta
    Commands
    Outputs
    • ITS2.fasta
    Execution Time 2016-12-2 19:0:11 (0.057 seconds)
  • align : Align FASTA files using MAFFT

    This stage aligns FASTA files (nucleotide or amino acid sequences) if required according input file status.

    Author nfranco@eafit.edu.co
    Inputs
    • ITS2.fasta
    Commands/home/bio/phypipe/scripts/apps/mafft/bin/mafft --quiet --auto ITS2.fasta > alignments/dna/ITS2.DNA.alignment.fasta
    Outputs
    • alignments/dna/ITS2.DNA.alignment.fasta
    Execution Time 2016-12-2 19:0:11 - 2016-12-2 19:0:13 (1.513 seconds)
  • reverse_translate : Convert amino acid alignment to nucleotides using RevTrans

    This stage transforms FASTA amino acid alignment file to nucleotide alignment matrix.

    Author nfranco@eafit.edu.co
    Inputs
    • alignments/dna/ITS2.DNA.alignment.fasta
    Commands
    Outputs
    • alignments/dna/ITS2.DNA.alignment.fasta
    Execution Time 2016-12-2 19:0:13 (0.025 seconds)
  • concat : Concatenate nucleotide alignments to single FASTA file

    This stage concatenates FASTA alignments in a single file. It also generates a configuration file for partitionfinder.

    Author nfranco@eafit.edu.co
    Inputs
    • /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/alignments/dna/18s.DNA.alignment.fasta
    • /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/alignments/dna/28s.DNA.alignment.fasta
    • /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/alignments/dna/58s.DNA.alignment.fasta
    • /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/alignments/dna/ITS1.DNA.alignment.fasta
    • /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/alignments/dna/ITS2.DNA.alignment.fasta
    Commands/home/bio/phypipe-run/venv/bin/python /home/bio/phypipe/scripts/concat_fasta_alignments.py /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/alignments/dna/18s.DNA.alignment.fasta /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/alignments/dna/28s.DNA.alignment.fasta /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/alignments/dna/58s.DNA.alignment.fasta /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/alignments/dna/ITS1.DNA.alignment.fasta /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/alignments/dna/ITS2.DNA.alignment.fasta /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/concat_matrix.fasta
    Outputs
    • /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/concat_matrix.fasta
    Execution Time 2016-12-2 19:0:40 (0.341 seconds)
  • convert : Convert concatenated FASTA file to NEXUS and Phylip files

    This stage creates NEXUS and Phylip files to be used in the downstream steps.

    Author nfranco@eafit.edu.co
    Inputs
    • /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/concat_matrix.fasta
    Commands/home/bio/phypipe-run/venv/bin/python /home/bio/phypipe/scripts/fasta2nexus.py /home/bio/Documents/Ctenophora_sequences/Arbol_18s_28s_58s_ITS1_ITS2/Arbol_mr_bayes_3_wi_Mnemiopsis/concat_matrix.fasta concat_matrix.nexus
    Outputs
    • concat_matrix.nexus
    Execution Time 2016-12-2 19:0:40 (0.365 seconds)
  • partition_finder : Find best partition scheme using partitionfinder

    This stage runs partitionfinder. It also creates MrBayes and garli blocks with best model information for each partition based on partitionfinder results.

    Author nfranco@eafit.edu.co
    Inputs
    • concat_matrix.nexus
    Commandscat concat_matrix.nexus phylo_analyses/garli/garli_pfinder.txt > phylo_analyses/garli/concat_w_partitions.nexus
    Outputs
    • phylo_analyses/garli/concat_w_partitions.nexus
    Execution Time 2016-12-2 19:0:40 - 2016-12-2 19:14:47 (14 minutes, 6.559 seconds)
  • phylo_analysis : Run phylogenetic analysis software

    This stage runs MrBayes or garli using files generated through the whole pipeline. It outputs consensus tree (for bayesian analysis) or best tree with bootstrap support (for maximum likelihood analysis)

    Author nfranco@eafit.edu.co
    Inputs
    • phylo_analyses/garli/concat_w_partitions.nexus
    • phylo_analyses/garli/concat_w_partitions.nexus
    Commandsmv phylo_analyses/garli/results.tre phylo_analyses/garli/phylo_maxlikelihood.tree
    Outputs
    • phylo_analyses/garli/phylo_maxlikelihood.tree
    Execution Time 2016-12-2 19:14:47 - 2016-12-3 12:8:1 (16 hours, 53 minutes, 13.655 seconds)